Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	239,802	C→T	E130K (GAA→AAA)	yafV ←	2‑oxoglutaramate amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	239,802	0	C	T	100.0%	208.5 / NA	60	E130K (GAA→AAA)	yafV	2‑oxoglutaramate amidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/31);  total (29/31)

TTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCCGCTGGTAAACATCTCCGGTAGAACGATCACATCGCGCCCGGTAAT  >  NC_000913/239514‑240096                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                         ttGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTACTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATCTCACAACCACt                                                                                                                                                                                                                                                                                                >  1:873/1‑299 (MQ=255)   gCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTcgc                                                                                                                                                                                                                                                                                             >  1:16851/1‑300 (MQ=255)                 ggCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCttt                                                                                                                                                                                                                                                                               >  1:638383/1‑300 (MQ=255)                      tAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAAt                                                                                                                                                                                                                                                                          <  1:38765/300‑1 (MQ=255)                             aaTCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATg                                                                                                                                                                                                                                                                   <  1:400159/300‑1 (MQ=255)                              aTCTCTACTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGc                                                                                                                                                                                                                                                                  >  2:208519/1‑300 (MQ=255)                                        gCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCa                                                                                                                                                                                                                                                        <  1:407762/300‑1 (MQ=255)                                                aTCACCCGGCTGTCACCGCGATAATTGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAAc                                                                                                                                                                                                                                                <  2:134287/300‑1 (MQ=255)                                                    cccGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATANGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                                                                                                                                                                                                                                            >  1:581177/1‑300 (MQ=255)                                                          tGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGc                                                                                                                                                                                                                                       <  1:130520/299‑1 (MQ=255)                                                             cACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTAt                                                                                                                                                                                                                                   >  2:136723/1‑300 (MQ=255)                                                                          gCAGCCGTTGCCATCGCTGCCGACGCAATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTAc                                                                                                                                                                                                                      <  2:455765/300‑1 (MQ=255)                                                                                             gcgagatgtgtataagagacagcaTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGAACACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAAcc                                                                                                                                                                                                   >  1:234625/23‑300 (MQ=255)                                                                                               gACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAAcc                                                                                                                                                                                                   <  1:216800/298‑1 (MQ=255)                                                                                                                  acaTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGNAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGaa                                                                                                                                                                              >  2:617105/1‑300 (MQ=255)                                                                                                                    aTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTAATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAAcc                                                                                                                                                                            <  1:773284/300‑1 (MQ=255)                                                                                                                        gCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCC‑GCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGAc                                                                                                                                                                        <  1:646894/299‑1 (MQ=255)                                                                                                                             aTTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGt                                                                                                                                                                   >  1:466128/1‑300 (MQ=255)                                                                                                                             aTTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAACCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGt                                                                                                                                                                   >  1:218425/1‑300 (MQ=255)                                                                                                                                  cAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTa                                                                                                                                                              >  2:13146/1‑300 (MQ=255)                                                                                                                                       gcgcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGc                                                                                                                                                          <  2:676658/299‑1 (MQ=255)                                                                                                                                           gggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAacac                                                                                                                                                     <  1:769062/300‑1 (MQ=255)                                                                                                                                                gTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGTCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACActgcc                                                                                                                                                 <  2:66658/299‑1 (MQ=255)                                                                                                                                                      aaTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTTAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATc                                                                                                                                        <  2:707902/300‑1 (MQ=255)                                                                                                                                                      aaTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑At                                                                                                                                         <  2:466128/299‑1 (MQ=255)                                                                                                                                                       aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCa                                                                                                                                       >  2:680016/1‑300 (MQ=255)                                                                                                                                                       aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCa                                                                                                                                       >  1:470967/1‑300 (MQ=255)                                                                                                                                                         gcctgccAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGTCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTGNTAATGTAGATGCTCATCTGCCATGCGGAACAGNTGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAgc                                                                                                                                     <  2:588555/300‑1 (MQ=255)                                                                                                                                                            tgccAGTGCCGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGACAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGCTGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAACAATGTACCGTGCAGCCCGGCTCAACCAGCAAAACGCGGTTACCCGCACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCa                                                                                                                                  >  1:782390/1‑300 (MQ=255)                                                                                                                                                            tgccAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGGTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATCATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAgcgc                                                                                                                                   <  2:537044/299‑1 (MQ=255)                                                                                                                                                              ccAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCAtt                                                                                                                                >  1:314775/1‑300 (MQ=255)                                                                                                                                                                aGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGc                                                                                                                              >  1:441127/1‑300 (MQ=255)                                                                                                                                                                  tGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCTCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCAc                                                                                                                            <  2:65036/300‑1 (MQ=255)                                                                                                                                                                  tGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCAc                                                                                                                            <  1:469575/300‑1 (MQ=255)                                                                                                                                                                  tGCAGAGAGCGCGGAGCAGGCCAG‑GTGGCGACGTACAGTGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCAc                                                                                                                            <  1:584854/300‑1 (MQ=255)                                                                                                                                                                       agagCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCActgctg                                                                                                                       <  2:787490/300‑1 (MQ=255)                                                                                                                                                                          gcgcGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTgcgc                                                                                                                    >  2:521605/1‑300 (MQ=255)                                                                                                                                                                            gcgGAGCAGGCCANCTTGGCGACGTACAGGGCNCAGGTCAGCGAATCGTTGGGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGCCNCATCAGCGCATTGCAc                                                                                                                            <  1:649516/296‑1 (MQ=255)                                                                                                                                                                            gcgGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGcc                                                                                                                   <  1:136723/299‑1 (MQ=255)                                                                                                                                                                            gcgGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCt                                                                                                                  <  1:707391/300‑1 (MQ=255)                                                                                                                                                                                         g‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCa                                                                                                     >  2:518403/1‑300 (MQ=255)                                                                                                                                                                                         g‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAAACAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCa                                                                                                     >  2:518379/1‑300 (MQ=255)                                                                                                                                                                                                   gTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTAc                                                                                            >  1:493449/1‑300 (MQ=255)                                                                                                                                                                                                   gTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCATTAGCTGTCATCCAGTTCACTAc                                                                                            >  1:731395/1‑300 (MQ=255)                                                                                                                                                                                                        gggCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCGGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCAt                                                                                       >  2:676040/1‑300 (MQ=255)                                                                                                                                                                                                        gggCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCACGCTTTATAATGTAGATGCTCATCTGCCATACGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAAACAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCATGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCAt                                                                                       >  2:676052/1‑300 (MQ=255)                                                                                                                                                                                                         ggCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGt                                                                                          <  1:21926/296‑1 (MQ=255)                                                                                                                                                                                                               ggTCA‑‑TAATCGTTGAGCTTGCGCGACCACACCGGAAAACGTCCGTCGTAGCACACCAGCGGCCAAATACGCCAGCCGCGCCATTTCACACTCACTCGCGCATTGCCCGCTTTCTAATGTAGCTGCTCATCTGCCATGCGGAACAGATGACGCTTCTCATAACAATGTACCGTGCCGCCCGTCTCAACCAGCAAAAAGCGGTTAACCGCACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATAAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCTCGTTCACTAAGTCATCtttt                                                                                  >  2:85395/1‑297 (MQ=255)                                                                                                                                                                                                               ggTCA‑‑TAATCATTGAGATTGCGCGACCACACAGGAACACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCTCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGATTCAGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGATGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTtgt                                                                                  >  2:85174/1‑299 (MQ=255)                                                                                                                                                                                                                                        gACCACACAGGAAAACGTAAGTCGTAGCACACCAGTGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTcc                                                          <  1:724339/300‑1 (MQ=255)                                                                                                                                                                                                                                        gACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTcc                                                          <  1:724327/300‑1 (MQ=255)                                                                                                                                                                                                                                           cacacaGGAAAACGTAAGTCGTAGCATACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATTTGCCATGCGGACCAGATGACGCTTATCATAAAAATGTACCGTGCCACCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGTATTGCACTGCTGCGCATTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGATGAAGCTGCCGCTTctat                                                        >  1:416908/1‑296 (MQ=255)                                                                                                                                                                                                                                            acacaGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCAt                                                        >  1:707845/1‑298 (MQ=255)                                                                                                                                                                                                                                                             tCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACATCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCAGCTGGTaaa                                      >  2:344247/1‑299 (MQ=255)                                                                                                                                                                                                                                                                            ggCAAAATACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCCGCTGGTAAACATCTCCGTTAGAAc                       <  2:754085/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   tACGCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCCGCTGGTAAACATCTCCGGTAGAACGATCACAt               <  1:46193/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCCGCTGGTAAACATCTCCGGTAGAACGATCACATcg             <  1:761573/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCATGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCCGCTGGTAAACATCTCCGGTAGAACGATCACATcg             <  2:25136/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gCCAGCCGCGCCATTTCACAATCACTCGCGCATTGCCCGCTTGATAATTTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCCGCTGGTAAACATCTCCGGTAGAACGATCACATcg             <  2:16851/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ccATTTCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCCGCTGGTAAACATCTCCGGTAGAACGATCACATCGCGCCCGGTAAt  >  2:441527/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                         TTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAG‑TTGGCGACGTACAGGGCG‑AGGTCA‑‑TAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCGCCCGGCTCAACCAGCAAAAAGCGGTTAACCGAACCAGACTCCGTTTGTAATGCAACACTGCCTGC‑A‑ATCAGCGCATTGCACTGCTGCGCCTTAGCTGTCATCCAGTTCACTACGTCATCTTGTGCTAGCGACGAAGCTGCCGCTTCCATGGCAAAGCCGCTGGTAAACATCTCCGGTAGAACGATCACATCGCGCCCGGTAAT  >  NC_000913/239514‑240096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑