Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,513,529	C→T	K260K (AAG→AAA)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,513,529	0	C	T	100.0%	161.1 / NA	48	K260K (AAG→AAA)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/26);  total (22/26)

GCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGCTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCC  >  NC_000913/4513241‑4513821                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                     gCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGTAAACAGGAATGGCCGAGTAGCAAANCAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCgcggc                                                                                                                                                                                                                                                                                           <  2:605772/300‑1 (MQ=255)           ttctgttggCGTTGGCGTGGGTCCAGCCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAAc                                                                                                                                                                                                                                                                                    <  2:266122/289‑1 (MQ=255)             cTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGTAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCt                                                                                                                                                                                                                                                                                >  1:215047/1‑299 (MQ=255)               tgTAGTGTTGGCGTGTGTCCAGGCAAAAGGGCGCGGGTGGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACTGGAAGCCCCCACATCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCTGCTGATGGTGTAAGATTTTTACCGTGTCAGCGGCAATATGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTACTGTTTCTGCGCGGCAGTTAACATCTGcc                                                                                                                                                                                                                                                                             <  2:381424/299‑1 (MQ=255)               ggTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTTTTTTATCGCGGTCATTTCACAACGGTAAGCTGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGcc                                                                                                                                                                                                                                                                             <  2:61199/300‑1 (MQ=255)               ggTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTACATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAGTAATAAGTGCTGCAGCGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAGCATCTGcc                                                                                                                                                                                                                                                                             <  2:381326/300‑1 (MQ=255)                    cGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCAAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTCAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCACCCTGCTGTTTCt                                                                                                                                                                                                                                                                                                  >  2:38555/1‑274 (MQ=255)                              tgtCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGNTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAAACTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCttgtt                                                                                                                                                                                                                                                              >  2:581330/1‑300 (MQ=255)                                      cagcagGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGt                                                                                                                                                                                                                                                      >  1:101234/1‑300 (MQ=255)                                           gggCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTANCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAAc                                                                                                                                                                                                                                                 <  2:579844/300‑1 (MQ=255)                                                 gggTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGTTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCACGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGc                                                                                                                                                                                                                                            <  2:2835/299‑1 (MQ=255)                                                                                        aaaaCAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGAt                                                                                                                                                                                                     <  1:680257/299‑1 (MQ=255)                                                                                              ctgcgagatgtgtataagagacaggTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGc                                                                                                                                                                                              >  1:144734/25‑300 (MQ=255)                                                                                                                           gcAACGGGAAGCCCACACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAAACAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCa                                                                                                                                                                 >  2:721203/1‑300 (MQ=255)                                                                                                                                gggAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGTTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGAc                                                                                                                                                            <  2:453977/300‑1 (MQ=255)                                                                                                                                 ggAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACg                                                                                                                                                           <  1:61020/300‑1 (MQ=255)                                                                                                                                 ggAAGCCCCCACAGCAGCACCGGGTGTTTCATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCTAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACg                                                                                                                                                           <  2:54068/300‑1 (MQ=255)                                                                                                                                       ccccACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCAccc                                                                                                                                                      >  1:619224/1‑299 (MQ=255)                                                                                                                                       ccccACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCAAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCAccc                                                                                                                                                      >  2:149212/1‑299 (MQ=255)                                                                                                                                                  caCCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGc                                                                                                                                           >  2:757821/1‑299 (MQ=255)                                                                                                                                                                     gTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCTGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCagag                                                                                                                       <  1:102160/300‑1 (MQ=255)                                                                                                                                                                     gTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCagag                                                                                                                       <  1:71855/300‑1 (MQ=255)                                                                                                                                                                       cATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCagaga                                                                                                                      >  1:763833/1‑299 (MQ=255)                                                                                                                                                                        aTTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCagag                                                                                                                       <  1:70365/297‑1 (MQ=255)                                                                                                                                                                           tCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCa                                                                                                                 >  1:104480/1‑300 (MQ=255)                                                                                                                                                                           tCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAAACTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCAAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGcc                                                                                                                  >  2:480452/1‑299 (MQ=255)                                                                                                                                                                               aaCGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGNGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCAc                                                                                                             <  1:565599/300‑1 (MQ=255)                                                                                                                                                                               aaCGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCAc                                                                                                             <  1:194569/300‑1 (MQ=255)                                                                                                                                                                                          gCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTcc                                                                                                  >  1:119938/1‑300 (MQ=255)                                                                                                                                                                                              aTGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGt                                                                                              <  1:632417/300‑1 (MQ=255)                                                                                                                                                                                               tgttggAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTTTCGACCTAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGtc                                                                                             <  1:97984/297‑1 (MQ=255)                                                                                                                                                                                               tggtggAAGATTTTTACCTTGTCAGCGGCAATACGCTCTGCAGTAAAAATACCGCGCATCCGCGCCCATGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGAGGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGtc                                                                                             <  1:98034/300‑1 (MQ=255)                                                                                                                                                                                               tggtggAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGtc                                                                                             <  1:98043/300‑1 (MQ=255)                                                                                                                                                                                                ggtggAAGATTTTTACCATGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTGTCTGCGCGGCGGTGAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTACTCCATGCCGATGGACgg                                                                                                                                                                   <  2:522383/229‑1 (MQ=255)                                                                                                                                                                                                         tttttACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGCCTACTGAGTAt                                                                                   >  2:104397/1‑300 (MQ=255)                                                                                                                                                                                                           tttACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCANCCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGc                                                                                 <  1:581330/300‑1 (MQ=255)                                                                                                                                                                                                                   gtCAGCGGCAATACGCTCTGCAGCAAAACTACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGTGGCGGTTAACATCTGCCAGAGTGGATCTTGTTGCCAGCGTTTAACACTGCTATCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTCGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGACGGTCCAGGTCTCCTGAGTATGCAGATTGaa                                                                         >  2:390473/1‑300 (MQ=255)                                                                                                                                                                                                                               aCGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAAACAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCCGGTCTCATGAGTATGCAGGTTGAATTGCTGTTcgc                                                              >  1:443965/1‑299 (MQ=255)                                                                                                                                                                                                                                    ctGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTACAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGa                                                          >  2:729517/1‑298 (MQ=255)                                                                                                                                                                                                                                        agcaAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGcc                                                    <  1:35388/300‑1 (MQ=255)                                                                                                                                                                                                                                         gcaAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGNGTTTAACAATGCTCTCTTCGCGATAGTGGGCANCCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCa                                                   >  2:582635/1‑300 (MQ=255)                                                                                                                                                                                                                                              aaTACCGCGCATCCGCGCCTAGGTTTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGTTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCATAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGc                                              <  1:550361/300‑1 (MQ=255)                                                                                                                                                                                                                                              aaTACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGc                                              <  1:178308/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gcgcATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCAATCGCAGCGGGAACGTTCAGCACCAGAGAGGACAGCACGCTGCCGGTCCAGGTCTAATGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCaagc                                         >  2:261316/1‑296 (MQ=255)                                                                                                                                                                                                                                                      gcATCCGCTCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATACTCTCTTCGCGATAGTGGGCAACCAGCAGCCATGCAGGATTGACCGCCAGCAGTTGCTCCATGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCAcgcg                                        <  2:111364/298‑1 (MQ=255)                                                                                                                                                                                                                                                         tCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTcc                                   >  1:137555/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gtgtTACTGTCGACCGAAGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAgc                        >  1:536956/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    aaGCAACCTGCTGTTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTgc         >  1:295513/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                            tgctgtTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATcc  <  1:553926/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                     GCACCAGCGCTTCTGGTAGCGTTGGCGTGTGTCCAGGCAGCAGGGCGCGGGTTGCATCTGCTCCGGAAACAGGAATGGCCGAGTAGCAAAACAGACTCAGCCAGAAAATAATAATAAGTGCTGCAACGGGAAGCCCCCACAGCAGCACCGGGTGTTTTATCGCGGTCATTTCACAACGGTAAGCGGCTGATGGTGGAAGATTTTTACCGTGTCAGCGGCAATACGCTCTGCAGCAAAAATACCGCGCATCCGCGCCCAGGTGTTACTGTCGACCGAAGCAACCTGCTGCTTCTGCGCGGCGGTTAACATCTGCCAGAGCGGATCTTGTTGCCAGCGTTTAACAATGCTCTCTTCGCGATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCC  >  NC_000913/4513241‑4513821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑