Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,399,260	G→A	S240F (TCC→TTC)	mreC ←	cell shape determining protein MreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,399,260	0	G	A	100.0%	223.7 / NA	64	S240F (TCC→TTC)	mreC	cell shape determining protein MreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/36);  total (28/36)

GTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCT  >  NC_000913/3398981‑3399538                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                       gcgagatgtgtataagagacaggTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGa                                                                                                                                                                                                                                                                    >  1:176767/22‑300 (MQ=255)       gtaTTTCCTGTCGCCGGGTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGGTAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCt                                                                                                                                                                                                                                                              <  2:189583/298‑1 (MQ=255)       gCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTTGCCCCATCGCGTCTGGCGAAGGCAATACCTGCTGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCNCCGTTACGATCTGCCCCCCACAGCAGCAGCAGNTAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCt                                                                                                                                                                                                                                                              <  2:594586/300‑1 (MQ=255)               tGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGa                                                                                                                                                                                                                                                       <  1:780992/299‑1 (MQ=255)                          gctgcGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGcaca                                                                                                                                                                                                                                           >  2:376112/1‑297 (MQ=255)                                gAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGACGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACCACCGCGACCGGCTAGCCTTCCGGGAAACGACCGCCCAGa                                                                                                                                                                                                                                         >  1:783419/1‑296 (MQ=255)                                  gTCGGCTTAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGaa                                                                                                                                                                                                                                   >  1:760409/1‑300 (MQ=255)                                        ttaGCGATCCCCGGTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTTCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTGAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCAcc                                                                                                                                                                                                                             <  2:435202/298‑1 (MQ=255)                                                    gTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCTGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCTGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCAc                                                                                                                                                                                                                   <  1:745808/298‑1 (MQ=255)                                                    gTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTNATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCAc                                                                                                                                                                                                                   <  1:168564/298‑1 (MQ=255)                                                     ttgccttttCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTTTGGCATCATCTGCATCGGACGTTCATTAGCAACACGATGCACCTCTTCCGTCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCACGCTGGGTATCGAGTTTTACTAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCACCCAGACCGGAAGTCACCAGCACATCACCaa                                                                                                                                                                                                                <  2:398296/293‑1 (MQ=255)                                                     ttGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCaa                                                                                                                                                                                                                <  1:553246/300‑1 (MQ=255)                                                           gTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGa                                                                                                                                                                                                           >  2:459387/1‑299 (MQ=255)                                                                 gTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCg                                                                                                                                                                                                    <  2:754658/300‑1 (MQ=255)                                                                    aCTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTNCGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATACCCAACACGAATGTTCGTAt                                                                                                                                                                                                 >  2:621653/1‑300 (MQ=255)                                                                    aCTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTAt                                                                                                                                                                                                 >  1:613829/1‑300 (MQ=255)                                                                      tttGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTc                                                                                                                                                                                               <  1:763158/300‑1 (MQ=255)                                                                               aTCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGNTAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGa                                                                                                                                                                                      >  1:565791/1‑300 (MQ=255)                                                                                                             atcatcTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCTGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCg                                                                                                                                                        <  1:65237/300‑1 (MQ=255)                                                                                                                   tGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc                                                                                                                                                   <  1:136306/299‑1 (MQ=255)                                                                                                                   tGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCg                                                                                                                                                  <  2:184776/300‑1 (MQ=255)                                                                                                                      aTCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCTTTACGATCTGCCCCCCACAGTAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCAGCCGTACGACCGTTACCGGc                                                                                                                                                <  2:381598/299‑1 (MQ=255)                                                                                                                              ttCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACACCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACCACCGTTACCGGCTGCCATTAc                                                                                                                                       >  2:279622/1‑300 (MQ=255)                                                                                                                              ttCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCAACAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAAtt                                                                                                                                         >  2:666293/1‑298 (MQ=255)                                                                                                                                   tAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACAAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTCCGCGGa                                                                                                                                  >  2:680201/1‑300 (MQ=255)                                                                                                                                          acGATGCACCTCTTCCTGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACNTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGt                                                                                                                            <  1:151073/299‑1 (MQ=255)                                                                                                                                              tGCACCTCTTCCTGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTgcgc                                                                                                                       <  2:665914/300‑1 (MQ=255)                                                                                                                                              tGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCNGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTgcgc                                                                                                                       <  1:555876/300‑1 (MQ=255)                                                                                                                                              tGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTgcgc                                                                                                                       <  1:718878/300‑1 (MQ=255)                                                                                                                                                    tctTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCAc                                                                                                                  <  2:403069/299‑1 (MQ=255)                                                                                                                                                          ggCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGa                                                                                                             <  1:52885/298‑1 (MQ=255)                                                                                                                                                           tcGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAATCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCTGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGAtt                                                                                                           <  2:36692/298‑1 (MQ=255)                                                                                                                                                             gTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGc                                                                                                        >  1:413130/1‑300 (MQ=255)                                                                                                                                                             gTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAACGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGc                                                                                                        >  1:615341/1‑300 (MQ=255)                                                                                                                                                             gTCATCGGGGTAGCGCCGTTATGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCGGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGc                                                                                                        <  2:492930/300‑1 (MQ=255)                                                                                                                                                                aTCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGNGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAg                                                                                                      <  1:558625/299‑1 (MQ=255)                                                                                                                                                                aTCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCTGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAg                                                                                                      <  1:536065/299‑1 (MQ=255)                                                                                                                                                                aTCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAg                                                                                                      <  1:423322/299‑1 (MQ=255)                                                                                                                                                                    ggTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGt                                                                                                 >  1:768525/1‑300 (MQ=255)                                                                                                                                                                        aGCGCCGTTACGATCTGCCCCCCGCAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAATAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATGCGCCTGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGTAGCGCGTGGGt                                                                                             <  1:15718/300‑1 (MQ=255)                                                                                                                                                                        aGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGt                                                                                             <  1:15717/300‑1 (MQ=255)                                                                                                                                                                         gcgcCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACGTCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTc                                                                                            >  1:460019/1‑300 (MQ=255)                                                                                                                                                                         gcgcCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTc                                                                                            >  1:460069/1‑300 (MQ=255)                                                                                                                                                                            ccGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCAACAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGAGGATACTGTTGCGCAGTACCTGGATTGGCAGCGCGTGGGTCGc                                                                                          >  2:130578/1‑299 (MQ=255)                                                                                                                                                                              gTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATc                                                                                       >  1:106777/1‑300 (MQ=255)                                                                                                                                                                              gTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGAAGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATc                                                                                       >  2:422987/1‑300 (MQ=255)                                                                                                                                                                                     tCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATc                                                                                <  1:785303/300‑1 (MQ=255)                                                                                                                                                                                                cagcagcagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTTGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATcaca                                                                                    >  2:628065/1‑285 (MQ=255)                                                                                                                                                                                                        gcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTCTTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGAGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCa                                                             >  2:640144/1‑300 (MQ=255)                                                                                                                                                                                                                 gCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCNGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGa                                                    <  2:566971/300‑1 (MQ=255)                                                                                                                                                                                                                      cGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGcc                                               >  1:780580/1‑300 (MQ=255)                                                                                                                                                                                                                        ttGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGcca                                              >  1:3890/1‑299 (MQ=255)                                                                                                                                                                                                                            aGCCCTGCAGTCGGGCGCGTCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCaccacc                                         <  2:648968/300‑1 (MQ=255)                                                                                                                                                                                                                            aGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCaccacc                                         <  2:488514/300‑1 (MQ=255)                                                                                                                                                                                                                                  gCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCAACACCTGACca                                   >  1:282392/1‑300 (MQ=255)                                                                                                                                                                                                                                                        caGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACG‑GGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCCGACTAACAACACCTTTGTCGCTGATGAc            >  2:266108/1‑300 (MQ=255)                                                                                                                                                                                                                                                        caGTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCAGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAACGGCTGCAATTAAGCGGATATCGTTGAGCAGCACCTGGATTGGCAACTCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGc                                                      >  2:77795/1‑259 (MQ=255)                                                                                                                                                                                                                                                         aGTATAAGCGCGTTGGGTATCGAGTTTGACGAAATAGACAACCGCGACCGGATAGCCTTCCTGGAAACGACCGCCCAGACCGGAAGGCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATNCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCtgatg              <  2:247879/298‑1 (MQ=255)                                                                                                                                                                                                                                                          gTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGAcc           >  1:258504/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gTATAAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTAACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCtgatgaac           >  2:471303/1‑298 (MQ=255)                                                                                                                                                                                                                                                           tataAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCTGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCtgat               <  2:751287/295‑1 (MQ=255)                                                                                                                                                                                                                                                            ataAGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCTGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCgg         <  2:543187/300‑1 (MQ=255)                                                                                                                                                                                                                                                              aaGCGCGCTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCt       <  1:525589/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    gcTGGGTATCGAGTTTTACGAAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCt  <  1:784408/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                       GTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCCT  >  NC_000913/3398981‑3399538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑