Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,359,916	G→A	G240E (GGG→GAG)	gltD →	glutamate synthase subunit GltD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,359,916	0	G	A	100.0%	195.6 / NA	62	G240E (GGG→GAG)	gltD	glutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/35);  total (27/35)

AAACAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAA  >  NC_000913/3359624‑3360211                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                        aaaCAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGGTTACGATGCCGTGTTCCTTGGCGTCGAGGCTTAt                                                                                                                                                                                                                                                                                                  >  2:50136/1‑300 (MQ=255) aaaCAGACCGATAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGAACGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCAGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCAACGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGAAGTCCAGCTGGACGATCTGCCGAGTGATTACAATGCCGTGTTCCTTGGTGTCGAGACTTAt                                                                                                                                                                                                                                                                                                  >  2:49856/1‑300 (MQ=255)      gACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTTGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCTTCGAGACTTATCAGTc                                                                                                                                                                                                                                                                                             <  1:469584/300‑1 (MQ=255)      gACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTc                                                                                                                                                                                                                                                                                             <  1:469589/300‑1 (MQ=255)            tAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCNGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATgcg                                                                                                                                                                                                                                                                                       <  1:154694/300‑1 (MQ=255)            tAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATgcg                                                                                                                                                                                                                                                                                       <  1:539943/300‑1 (MQ=255)                         aTTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGaa                                                                                                                                                                                                                                                                          <  2:654884/300‑1 (MQ=255)                         aTTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGaa                                                                                                                                                                                                                                                                          <  1:372141/300‑1 (MQ=255)                              gcgcgcAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGa                                                                                                                                                                                                                                                                     >  1:414088/3‑300 (MQ=255)                                             aGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCg                                                                                                                                                                                                                                                       <  1:739357/299‑1 (MQ=255)                                              ggTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCgt                                                                                                                                                                                                                                                      >  2:373003/1‑299 (MQ=255)                                              ggTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCgt                                                                                                                                                                                                                                                      >  1:597857/1‑299 (MQ=255)                                                  tGGCGTGTGCGGATGGCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGTCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTNAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGATTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTAc                                                                                                                                                                                                                                                  <  2:565453/299‑1 (MQ=255)                                                   ggCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGc                                                                                                                                                                                                                                                <  2:740146/300‑1 (MQ=255)                                                      gtgtGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTAGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAg                                                                                                                                                                                                                                              <  1:309129/299‑1 (MQ=255)                                                             gATGTCCTGACGCGTAAAGGCGTAAAAGCAGTTGTCTTCGAACGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTAAAGCTGGGAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAACTCAAACTCAATACCGAAGTGGGCCGCAACGTACAGCTGAACGATCTGTTGAGTGATTACGATGCCTTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGCTGGCGTGTACGCAgc                                                                                                                                                                                                                                             >  2:389224/1‑293 (MQ=255)                                                                gTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTc                                                                                                                                                                                                                                   >  2:750930/1‑300 (MQ=255)                                                                gTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTAAACGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTCCAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCAGATGGCGTGTACGCAGCGCTGCCGTTc                                                                                                                                                                                                                                   >  2:286824/1‑300 (MQ=255)                                                                gTCCTGACGCGTAACGGCGTAAAAGCCGTTGTATTCGAACGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCAGTGAAATCTTCAACGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGAAGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGACGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGCGTACGccaagctaccgttc                                                                                                                                                                                                                                   >  2:286925/1‑287 (MQ=255)                                                                                gCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCa                                                                                                                                                                                                                   >  2:202395/1‑300 (MQ=255)                                                                                           gTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTaagg                                                                                                                                                                                                        <  1:764333/300‑3 (MQ=255)                                                                                              gTCTTCGACCGTCATCCAGAAATTGGCGTGCTGCTGACCTTCGTTATTCCGGCCTTCAAGCGGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCTTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGc                                                                                                                                                                                                     <  1:480667/300‑1 (MQ=255)                                                                                              gTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGc                                                                                                                                                                                                     >  1:609143/1‑300 (MQ=255)                                                                                              gTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATCCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGc                                                                                                                                                                                                     >  1:609218/1‑300 (MQ=255)                                                                                                     aCCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTGCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTg                                                                                                                                                                                              <  2:635775/300‑1 (MQ=255)                                                                                                           aTCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCTGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAAcc                                                                                                                                                                                         <  1:420052/299‑1 (MQ=255)                                                                                                           aTCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAAcc                                                                                                                                                                                         <  1:771415/299‑1 (MQ=255)                                                                                                            tCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGAGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAAccc                                                                                                                                                                                        >  2:371970/1‑299 (MQ=255)                                                                                                                            ctgctgACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGNTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACAACAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGt                                                                                                                                                                        >  2:572360/1‑299 (MQ=255)                                                                                                                            ctgctgACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTc                                                                                                                                                                       >  1:459813/1‑300 (MQ=255)                                                                                                                              gctgACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGTTAATTACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACTGTTCGTCAg                                                                                                                                                                     <  2:448906/300‑1 (MQ=255)                                                                                                                                         gTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGNTGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCa                                                                                                                                                          >  2:565357/1‑300 (MQ=255)                                                                                                                                           aTTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCaaa                                                                                                                                                        <  1:425436/300‑1 (MQ=255)                                                                                                                                                             gAAAAAGAGGTAATGACGCGTCGCCGGGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGTGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGc                                                                                                                                      <  2:130648/300‑1 (MQ=255)                                                                                                                                                                      gTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGac                                                                                                                             >  1:196582/1‑300 (MQ=255)                                                                                                                                                                                   gCCGTTAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGTTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCGCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                 <  2:37087/299‑1 (MQ=255)                                                                                                                                                                                   gCCGTGAAATCTTCAGCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                 <  1:417690/299‑1 (MQ=255)                                                                                                                                                                                   gCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                 <  1:79427/299‑1 (MQ=255)                                                                                                                                                                                   gCCGTGAAATCTTCACCGGCATGGGTATTGAATGCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                 <  1:755830/299‑1 (MQ=255)                                                                                                                                                                                        gAAATCTTCACCTGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                 <  1:215932/294‑1 (MQ=255)                                                                                                                                                                                            tCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCANCGCTGCCGTTCCTCATCGCCAACACCAAACAGNTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTa                                                                                                       <  2:597857/300‑1 (MQ=255)                                                                                                                                                                                            tCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTa                                                                                                       <  1:264769/300‑1 (MQ=255)                                                                                                                                                                                                 aCCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGGGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTc                                                                                                   <  2:473731/299‑1 (MQ=255)                                                                                                                                                                                                    tgCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCg                                                                                                 <  1:334344/297‑1 (MQ=255)                                                                                                                                                                                                    ggCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCg                                                                                                 <  1:333757/298‑1 (MQ=255)                                                                                                                                                                                                    ggCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCAGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAAAGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCACCAACAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGt                                                                                                >  1:214325/1‑299 (MQ=255)                                                                                                                                                                                                         gggTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTACTGGGCTTTGGTGACACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCTTGGTGGTCCTTGTCGGTGGCGACCCTGCGATGGACTGCGTGCGTACGTCCGTGCGCCa                                                                                          >  2:376406/1‑300 (MQ=255)                                                                                                                                                                                                           gTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCa                                                                                          >  1:108043/1‑298 (MQ=255)                                                                                                                                                                                                            tATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAggg                                                                                       <  1:221825/300‑1 (MQ=255)                                                                                                                                                                                                               tGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGc                                                                                    <  1:306252/300‑1 (MQ=255)                                                                                                                                                                                                                             aTACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCt                                                                      >  2:717209/1‑300 (MQ=255)                                                                                                                                                                                                                                            gcgACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATg                                                                                                                                                                                                        <  1:446377/155‑1 (MQ=255)                                                                                                                                                                                                                                            gcgACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATg                                                                                                                                                                                                        >  2:446377/1‑155 (MQ=255)                                                                                                                                                                                                                                              gACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGtgat                                                     <  2:196582/300‑1 (MQ=255)                                                                                                                                                                                                                                               aCGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCANCACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGtgat                                                     >  2:563414/1‑299 (MQ=255)                                                                                                                                                                                                                                               aCGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACAACAAACAGTTAATGGGCTTTGGTGAAAACCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGtgat                                                     >  2:5510/1‑299 (MQ=255)                                                                                                                                                                                                                                                  tACAGCTGGACGATCTGCTGAGTGATTACGATGCCGGGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAag                                                 <  1:285095/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGaa                                             >  2:767553/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gctgAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTc                                 >  2:105627/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 gTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGgaagaa  >  2:469213/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 gTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGgaagaa  <  1:50136/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCAACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGgaagaa  <  1:49856/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                        AAACAGACCGGTAAAAAAGTGGCGATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAA  >  NC_000913/3359624‑3360211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑