Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,027,752	G→A	L341L (CTG→TTG)	fadA ←	3‑ketoacyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,027,752	0	G	A	100.0%	209.5 / NA	64	L341L (CTG→TTG)	fadA	3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/21);  total (43/21)

CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCTAATTCATGGGCGCGGCTTTCACTCATCACCAGCATGGCAGCTGCGCCATC  >  NC_000913/4027468‑4028046                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                       ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCAcc                                                                                                                                                                                                                                                                                         >  1:468952/1‑300 (MQ=255) ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGAACCAGACCGATACACATCGTCGCAAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAAACCAGCGGATGACCAAACGCGATcgcgac                                                                                                                                                                                                                                                                                            >  2:74011/1‑295 (MQ=255) ccGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGAAATACCCTGACCCAGACCGATACACCTCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAAAGCGATCGCGCCaac                                                                                                                                                                                                                                                                                         >  1:469016/1‑298 (MQ=255)           gATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGtt                                                                                                                                                                                                                                                                                >  1:305731/1‑299 (MQ=255)                                      aTTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGt                                                                                                                                                                                                                                                    >  2:512317/1‑300 (MQ=255)                                           tGCATGATGCCTGATGCGACGCTTGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTTACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTc                                                                                                                                                                                                                                                   <  2:460231/296‑1 (MQ=255)                                                      tGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTACATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATAc                                                                                                                                                                                                                                    <  1:592759/300‑1 (MQ=255)                                                                          ttATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCa                                                                                                                                                                                                                >  1:197105/1‑300 (MQ=255)                                                                           tATCAGGCCTACATTGGTGCCGAATCGGTAGACCGGATAATGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCATCGGATGACCCAACGCGATCGCGCCACCGGTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTgcgc                                                                                                                                                                                                                    <  2:408416/295‑1 (MQ=255)                                                                                ggCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCt                                                                                                                                                                                                          >  1:296054/1‑300 (MQ=255)                                                                                 gCCTCCATTGGTGCCGGATCGGTAGACCGGATAAAGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTAGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCAATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCACTTAGTACCCGATCTTTAATACATGGCAGAATCTGCGCGGCAAAGGCtt                                                                                                                                                                                                         >  2:151958/1‑300 (MQ=255)                                                                                   ctaaaTTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTAAGCAGCGTGGTGCTGATAAGCGCACCGGAGCAACCAAGCGGATGAACAAACACGATCGCGCCAACGGTGAGGTTGATCTTGTCGTCAATCTGCTCAACTAGTCCCAGATCTTTAATACATGGCAGGGTCTGCGCGGCaaa                                                                                                                                                                                                              >  2:73106/5‑293 (MQ=255)                                                                                   cTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGCCCAAACTGAACGTCTTTGCGTTCCATCAAATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTGTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCa                                                                                                                                                                                                                >  2:73107/1‑291 (MQ=255)                                                                                   cTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTc                                                                                                                                                                                                        >  1:53356/1‑299 (MQ=255)                                                                                     aCATTGGTGCCGGATTGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACTGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATGCGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAATGCTTCGtt                                                                                                                                                                                                     <  2:457313/300‑1 (MQ=255)                                                                                     aCATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGtt                                                                                                                                                                                                     <  1:513575/300‑1 (MQ=255)                                                                                      cATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGNTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTc                                                                                                                                                                                                    >  1:567939/1‑300 (MQ=255)                                                                                       aTTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCGAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCa                                                                                                                                                                                                   >  1:418600/1‑300 (MQ=255)                                                                                                ggATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGAGTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCATCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTGGTCCCAGATCTTTAATACATTGCAGGATCTGCGCTGCAAAGGCTTCGTTCATTTCAAaca                                                                                                                                                                                          <  2:111437/300‑1 (MQ=255)                                                                                                             ggATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTNAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCt                                                                                                                                                                             >  1:554108/1‑300 (MQ=255)                                                                                                             ggATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCt                                                                                                                                                                             >  1:717282/1‑300 (MQ=255)                                                                                                              gATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCt                                                                                                                                                                             >  1:599313/1‑299 (MQ=255)                                                                                                                                cATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGAt                                                                                                                                                                                                                                             >  1:378160/1‑217 (MQ=255)                                                                                                                                cATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGAt                                                                                                                                                                                                                                             <  2:378160/217‑1 (MQ=255)                                                                                                                                           tGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAgcgcaa                                                                                                                                                >  2:373613/1‑297 (MQ=255)                                                                                                                                            ggTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGt                                                                                                                                              >  1:701176/1‑300 (MQ=255)                                                                                                                                            ggTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCAGCTTTTTTCAGCGCCAGt                                                                                                                                              >  2:449957/1‑300 (MQ=255)                                                                                                                                                       cTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGca                                                                                                                                     >  1:496471/1‑297 (MQ=255)                                                                                                                                                       cTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGCCTTTGCGTTCCATCAGATTCAGCCGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCAACGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAAGATCTGCGCGGCaaa                                                                                                                                                                                                              >  2:29861/1‑225 (MQ=255)                                                                                                                                                                  tCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGt                                                                                                                        >  1:509712/1‑300 (MQ=255)                                                                                                                                                                    gtaaTACCCTGACCCAGACCGATACACATCGTCGACAGACCAAACTGAACATCTTTGCATTCCCTCAGATTCAGCAGCGCGATGCTGCTACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCAGCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCAAGATCTTTAATACATGGCAGGATTTGCGCGGCAAAGGATTCGTTCATTTCACACACGCCGATATCGCTGGCAGAAAGCCCCGCTCTTTTCAACGCCAGTTTCGAGGCAGGAACCGGGc                                                                                                                           >  2:647836/3‑295 (MQ=255)                                                                                                                                                                    gCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGTATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTaa                                                                                                                      <  2:363340/300‑1 (MQ=255)                                                                                                                                                                    gCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTaa                                                                                                                      >  1:252480/1‑300 (MQ=255)                                                                                                                                                                      aaTACCCTTACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCNCAATTAGTCCCAGATCTTTAATACATGGCAGGNTCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAAc                                                                                                                     <  2:595535/299‑1 (MQ=255)                                                                                                                                                                         aCCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGNTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCAt                                                                                                                 >  2:606187/1‑300 (MQ=255)                                                                                                                                                                             tGACCCAGACCGATACACATCGTCGCCATACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAAt                                                                                                              <  1:529808/299‑1 (MQ=255)                                                                                                                                                                             tGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCTTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAAt                                                                                                              <  2:778371/299‑1 (MQ=255)                                                                                                                                                                             tGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAAt                                                                                                              <  1:539635/299‑1 (MQ=255)                                                                                                                                                                             tGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATc                                                                                                             <  2:679640/300‑1 (MQ=255)                                                                                                                                                                               aCCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGa                                                                                                           >  1:100765/1‑300 (MQ=255)                                                                                                                                                                                   aGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATg                                                                                                         <  1:276271/298‑1 (MQ=255)                                                                                                                                                                                    gACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGt                                                                                                      >  1:56475/1‑300 (MQ=255)                                                                                                                                                                                      ccGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATTGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCCTTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTca                                                                                                    >  2:504628/1‑300 (MQ=255)                                                                                                                                                                                      ccGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTca                                                                                                    >  1:551498/1‑300 (MQ=255)                                                                                                                                                                                          tACACATCGTCGCCAGACCAAACTAAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGAATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCGTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCAcaac                                                                                                <  2:34920/300‑1 (MQ=255)                                                                                                                                                                                                         gACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCACCGACCGCCATc                                                                                  >  1:592849/1‑299 (MQ=255)                                                                                                                                                                                                                       tCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAgc                                                                    >  1:646334/1‑299 (MQ=255)                                                                                                                                                                                                                       tCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAgc                                                                    >  2:681871/1‑299 (MQ=255)                                                                                                                                                                                                                         tttGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAg                                                                     <  2:651072/296‑1 (MQ=255)                                                                                                                                                                                                                                 ccATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCtcaa                                                         >  2:751688/1‑297 (MQ=255)                                                                                                                                                                                                                                 ccATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTaa                                                         >  1:493741/1‑300 (MQ=255)                                                                                                                                                                                                                                      aGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCt                                                    >  1:596957/1‑300 (MQ=255)                                                                                                                                                                                                                                                gcGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGGTCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCNCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCAAACCCAACGACCGCCATCGAACGAACACGa                                                                      >  2:248769/1‑272 (MQ=255)                                                                                                                                                                                                                                                gcGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCAACGTTGAGGTTGATCTTCTAGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCACGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCNCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTAACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCTAATTCAt                                             >  2:248744/1‑297 (MQ=255)                                                                                                                                                                                                                                                  gtggtgCTGATACGCGCACCGGAACAACCCATCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAGCGCACACGAGCGCGCGGCTTAAGACCTAATTCATggg                                          <  1:620382/298‑1 (MQ=255)                                                                                                                                                                                                                                                  gtggtgCGGATACGCGCACAGGAACAACCCAGCGGGTGACCCAACGAGATCGCGTCACCGTTGAGGTGGATCTTTTCGTCAATCTGCGCACTTAGTCCCAGACCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGTCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCACCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACGACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTGAAGACCGAATTCGTGGgc                                         <  1:771376/299‑1 (MQ=255)                                                                                                                                                                                                                                                     gtgCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTAGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCTAATTCATGGgcgc                                       >  1:521396/1‑298 (MQ=255)                                                                                                                                                                                                                                                             aCGCGCACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATTGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCTAATTCATGGGCGCGGCTTTCACt                             <  1:638890/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  cACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCAGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGAACTAATTCATGGATGCGGCTTTCACtcatca                        >  2:555/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  cACCGGAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTAGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGAACGCCATCGAACGCACACGAGCGCGAGGATTAAGAACTAATTCATGGGTGCGGCTTTAACt                             >  2:676/1‑295 (MQ=255)                                                                                                                                                                                                                                                                      ggAACAACCCAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCTAATTCATGGGCGCGGCTTTCACTCATCAcc                      <  1:400952/298‑1 (MQ=255)                                                                                                                                                                                                                                                                              ccAGCGGATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGGAAAGGCCTCATTCATTTCacggct                                                                                                                                                                                         >  2:684665/1‑122 (MQ=255)                                                                                                                                                                                                                                                                                    gATGACCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCTAATTCATGGGCGCGGCTTTCACTCATCACCAGCATGGCAGCTgcgc      <  1:329624/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        aCCCAACGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCTAATTCATGGGCGCGGCTTTCACTCATCACCAGCATGGCAGCTGCGCCATc  >  2:487145/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                       CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGATCGCGCCACCGTTGAGGTTGATCTTCTCGTCAATCTGCTCAATTAGTCCCAGATCTTTAATACATGGCAGGATCTGCGCGGCAAAGGCTTCGTTCATTTCAAACACGCCGATATCGCTGGCAGAAAGCCCCGCTTTTTTCAGCGCCAGTTTCGAGGCCGGAACCGGGCCGTAACCCATAATCGATGGGTCACAACCAACGACCGCCATCGAACGCACACGAGCGCGCGGCTTAAGACCTAATTCATGGGCGCGGCTTTCACTCATCACCAGCATGGCAGCTGCGCCATC  >  NC_000913/4027468‑4028046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑