Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,033,621	G→A	Q159Q (CAG→CAA)	trkH →	K(+) transporter TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,033,621	0	G	A	100.0%	324.1 / NA	94	Q159Q (CAG→CAA)	trkH	K(+) transporter TrkH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (61/33);  total (61/33)

GGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTCCGGCTGTAACTACGGTCTGCACTTT  >  NC_000913/4033328‑4033915                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       ggCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTAt                                                                                                                                                                                                                                                                                                  <  1:588810/300‑1 (MQ=255)     aaCTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATcgc                                                                                                                                                                                                                                                                                               >  1:478490/1‑299 (MQ=255)      aCTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCgcgc                                                                                                                                                                                                                                                                                             <  1:755984/300‑1 (MQ=255)                     ggtgTTTCTGAGAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCTGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGGGGGGTTGGATTCGCTCCCTCACGCCATCCTCTTTTATCTCCAGATGCTGCAAGGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGAGACTGCCTATCCTCGGCGTGGGTGGGAGGCAACTCTACCGCCCAGAAATgccc                                                                                                                                                                                                                                                                                   <  2:773632/292‑1 (MQ=255)                        gTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCt                                                                                                                                                                                                                                                                           >  1:731000/1‑300 (MQ=255)                            cTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGaaa                                                                                                                                                                                                                                                                       >  1:454731/1‑300 (MQ=255)                                     gtgCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACaaa                                                                                                                                                                                                                                                              >  1:333794/1‑300 (MQ=255)                                          gTTCTGGACCGTGCTGNGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGNAGAAATGCCCGGCCCGCTNAAAGATAACAAAATgc                                                                                                                                                                                                                                                          >  1:648845/1‑299 (MQ=255)                                          gTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAGTgc                                                                                                                                                                                                                                                          >  1:262332/1‑299 (MQ=255)                                          gTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATgc                                                                                                                                                                                                                                                          >  1:9347/1‑299 (MQ=255)                                          gTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAAATAACaaaacgc                                                                                                                                                                                                                                                          >  2:494050/1‑296 (MQ=255)                                          gTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCATGACCAATACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCATCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGGTGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAAACAAATgc                                                                                                                                                                                                                                                          >  2:60213/1‑299 (MQ=255)                                          gTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTATGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATgc                                                                                                                                                                                                                                                          >  2:288924/1‑299 (MQ=255)                                          gTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTAGGAAAGCCCGAACCTCAAGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGGTGGGGATCATCGTGTTGGCGGTTGCGCTACTTCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCTCGGCCCGCTGAAAGATAACAAAAt                                                                                                                                                                                                                                                            >  2:10302/1‑297 (MQ=255)                                          gTTCTGGACCGCGCTGGGTAGCGTCGGTGCGCTCCATTTTATCTTCTCGGAAAGCCCGAACCTCCCGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCAATCCTCTTTTATCGCCGGATGCTGCAATGGTTTGGCGGGGTGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTCTCCTCGGCATGGGTGGGATGCAACTCCATCGCGCAGAAATgc                                                                                                                                                                                                                                                                                     >  2:494006/1‑272 (MQ=255)                                                    gTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAAtt                                                                                                                                                                                                                                               >  1:535318/1‑300 (MQ=255)                                                    gTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCACGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAAtt                                                                                                                                                                                                                                               >  2:83859/1‑300 (MQ=255)                                                    gTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGACAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAAtt                                                                                                                                                                                                                                               >  2:657770/1‑300 (MQ=255)                                                    gTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCAACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCATATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAAtt                                                                                                                                                                                                                                               >  2:143332/1‑300 (MQ=255)                                                    gTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTAAGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGGTGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTACCTACCTTCGGCGTGGGTGGGATGCAAATCTATCGCGCAGAAATGCACGGCCCGCTGAAAGACAACAAAATGCACCcgaaaatt                                                                                                                                                                                                                                               >  2:83710/1‑294 (MQ=255)                                                    gTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTAGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCCGACATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGaact                                                                                                                                                                                                                                               >  2:43999/1‑298 (MQ=255)                                                    gTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTAGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCACGCGAAtt                                                                                                                                                                                                                                               >  2:320774/1‑300 (MQ=255)                                                         gggTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGa                                                                                                                                                                                                                                          >  1:698508/1‑300 (MQ=255)                                                               cGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGc                                                                                                                                                                                                                                    >  2:686633/1‑300 (MQ=255)                                                                           cccTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAAcc                                                                                                                                                                                                                             >  1:439858/1‑295 (MQ=255)                                                                              ttttATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGt                                                                                                                                                                                                                      <  2:141774/299‑1 (MQ=255)                                                                                        tcGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTc                                                                                                                                                                                                           <  1:14659/300‑1 (MQ=255)                                                                                                   gAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGAc                                                                                                                                                                                                   <  1:10361/297‑1 (MQ=255)                                                                                                      ccTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGc                                                                                                                                                                                             >  1:447550/1‑300 (MQ=255)                                                                                                        tCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGcc                                                                                                                                                                                            <  1:760604/299‑1 (MQ=255)                                                                                                           cGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGc                                                                                                                                                                                             >  1:625886/1‑295 (MQ=255)                                                                                                                   gATGCGTTGTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCGGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACTGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCg                                                                                                                                                                                <  2:119794/300‑1 (MQ=255)                                                                                                                    aTGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCTGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCg                                                                                                                                                                                <  2:119175/299‑1 (MQ=255)                                                                                                                    aTGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCg                                                                                                                                                                                <  2:138336/299‑1 (MQ=255)                                                                                                                                      tctcTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAAt                                                                                                                                                               >  2:180589/1‑298 (MQ=255)                                                                                                                                      tctatGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCATGTGGTTGATTTATGTCTTGCTGACCGTCGACTGTGCGCTGGCGTTGTGGTTTGCCGGAAt                                                                                                                                                               >  2:399345/5‑298 (MQ=255)                                                                                                                                                gACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGa                                                                                                                                                   >  1:673403/1‑300 (MQ=255)                                                                                                                                                gACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGa                                                                                                                                                   >  2:524570/1‑300 (MQ=255)                                                                                                                                                  ccACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGAt                                                                                                                                                  >  2:739766/1‑299 (MQ=255)                                                                                                                                                   cACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTNGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACTGTCGCCTGTGCGCTGGTGTTGTGGTTTGCCGGACTGGATGCCTTTGAttc                                                                                                                                                >  2:575505/1‑298 (MQ=255)                                                                                                                                                   cACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGc                                                                                                                                                >  2:172330/1‑300 (MQ=255)                                                                                                                                                    aCTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCATTTGATGcc                                                                                                                                               >  2:639009/1‑300 (MQ=255)                                                                                                                                                     cTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCCGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCNTGTGGTTGATTTATGTCTTGCTTACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCa                                                                                                                                              >  1:570616/1‑300 (MQ=255)                                                                                                                                                           gAGCCACTACGCTGGTTTGGCTGGATTCGCTCCCTCACGCCATCCTCCTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGGGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGcc                                                                                                                                        <  2:396930/300‑1 (MQ=255)                                                                                                                                                              ccACTACGCTTGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATa                                                                                                                                     >  1:468892/1‑300 (MQ=255)                                                                                                                                                              ccACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATa                                                                                                                                     >  1:224807/1‑300 (MQ=255)                                                                                                                                                              ccACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGACAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATa                                                                                                                                     >  1:621983/1‑300 (MQ=255)                                                                                                                                                                aCTACGCTGTTGTGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGTGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGc                                                                                                                                   <  2:204249/300‑1 (MQ=255)                                                                                                                                                                aCTACGCTGGTTGTGCTGGATTCGCTCCCTCACTCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGTCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGc                                                                                                                                   <  2:212354/300‑1 (MQ=255)                                                                                                                                                                aCTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGc                                                                                                                                   >  1:471535/1‑300 (MQ=255)                                                                                                                                                                   aCGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCttt                                                                                                                                >  2:522792/1‑300 (MQ=255)                                                                                                                                                                   aCGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCttt                                                                                                                                >  1:599036/1‑300 (MQ=255)                                                                                                                                                                   aCGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCATGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCttt                                                                                                                                >  1:201155/1‑300 (MQ=255)                                                                                                                                                                        ggtggGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGAc                                                                                                                           >  2:777865/1‑300 (MQ=255)                                                                                                                                                                             ggCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATc                                                                                                                       >  2:166764/1‑299 (MQ=255)                                                                                                                                                                             ggCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATc                                                                                                                       >  1:46974/1‑299 (MQ=255)                                                                                                                                                                                       gCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTggcg                                                                                                             <  1:462499/299‑1 (MQ=255)                                                                                                                                                                                          cccTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTNCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCCTAGCTTTGCGACTATTGCTATTGGCGGCtt                                                                                                         >  2:248476/1‑300 (MQ=255)                                                                                                                                                                                           ccTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTtc                                                                                                        >  1:634221/1‑300 (MQ=255)                                                                                                                                                                                               aCGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGa                                                                                                    >  2:751737/1‑300 (MQ=255)                                                                                                                                                                                                    aTCCTCTTTTATCGCCAGATGCTGCAATGTTTTTGCGGGATGTGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGacac                                                                                                 <  2:338062/298‑1 (MQ=255)                                                                                                                                                                                                    aTCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAANGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGacac                                                                                                 <  2:237569/298‑1 (MQ=255)                                                                                                                                                                                                    aTCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGacac                                                                                                 <  1:376388/298‑1 (MQ=255)                                                                                                                                                                                                        tctTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACatgat                                                                                            <  1:283816/299‑1 (MQ=255)                                                                                                                                                                                                             tagcGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGt                                                                                      <  2:477171/297‑1 (MQ=255)                                                                                                                                                                                                               tCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGNGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTAt                                                                                    <  1:166764/300‑1 (MQ=255)                                                                                                                                                                                                                      aTGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCCTCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGttct                                                                             >  2:300944/1‑298 (MQ=255)                                                                                                                                                                                                                      aTGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTAt                                                                             >  2:337106/1‑300 (MQ=255)                                                                                                                                                                                                                                         ggATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCCGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTAtt                                                           >  2:114538/1‑299 (MQ=255)                                                                                                                                                                                                                                           aTGGGGATCATCGTGTTTGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATTGCTATTGGTGGCGTCTCGACACATGAGGCCAGTATCGGTTATTTCGATAGCCCGACTATGAac                                                        <  1:678833/300‑1 (MQ=255)                                                                                                                                                                                                                                           aTGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAac                                                        <  2:731000/300‑1 (MQ=255)                                                                                                                                                                                                                                             ggggATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCCCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATANCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAacac                                                      >  1:158792/1‑300 (MQ=255)                                                                                                                                                                                                                                              gggATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCATGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACt                                                     >  1:325916/1‑300 (MQ=255)                                                                                                                                                                                                                                                 atcatcGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATc                                                  >  1:57264/1‑300 (MQ=255)                                                                                                                                                                                                                                                  tcatcGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCTGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCTACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCa                                                 <  1:687430/300‑1 (MQ=255)                                                                                                                                                                                                                                                     tcGTTTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTg                                              <  1:60213/300‑1 (MQ=255)                                                                                                                                                                                                                                                       gtgtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCt                                            <  1:199027/300‑1 (MQ=255)                                                                                                                                                                                                                                                          ttGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCTGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATc                                         <  2:447550/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 ttGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCct                                    >  2:108101/1‑298 (MQ=255)                                                                                                                                                                                                                                                                   gCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTCGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCctgctg                                <  2:535318/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      aTACTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGAtc                             >  1:692394/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         cTGCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGGTAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCNCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGAATATTAACACTATCATTGCTATCTTCCTGCTGATCTcc                          >  2:554957/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           gCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTCTGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTcc                          >  1:403577/1‑298 (MQ=255)                                                                                                                                                                                                                                                                           gCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTNGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTcc                          >  1:145894/1‑298 (MQ=255)                                                                                                                                                                                                                                                                           gCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTcc                          >  2:466480/1‑298 (MQ=255)                                                                                                                                                                                                                                                                           gCCTATCCTCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTcc                          >  1:400315/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                   tCGGCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTTTTGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTCCGGCTGTAACt                <  2:149910/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     ggCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTCCGGCTGTAAc                 <  2:434393/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gCGTGGGTGGGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTCCGGCTGTAACTAc              >  1:104018/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                        gtgggtggGATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTCCGGCTGTAACTACgg            <  1:143332/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gggtgggATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGTCGAAAACCCTTTGTTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTGTTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTCCGGCTGTAACTACGGTc          <  2:338864/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gggATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTCCGGCTGTAACTACGGTCTGCAc     <  1:300944/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               ggATGCAACTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGNCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCCGATCTCCGGCTGTAACTAAGGTCTGCACt    >  2:238076/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 aTGCAACTCTATCGCGCATAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTTGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTACTGATCTCCTGCTGTAACTACGGTCTGCACttt  <  1:457749/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                       GGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTTGAATCTTTCTCTGGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGAATGGATGCCTTTGATGCCATCGGCCATAGCTTTGCGACTATCGCTATTGGCGGCTTCTCGACACATGATGCCAGTATCGGTTATTTCGATAGCCCGACTATTAACACTATCATTGCTATCTTCCTGCTGATCTCCGGCTGTAACTACGGTCTGCACTTT  >  NC_000913/4033328‑4033915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑