Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,193,850	G→A	L119L (CTG→TTG)	thiE ←	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,193,850	0	G	A	100.0%	159.2 / NA	48	L119L (CTG→TTG)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/28);  total (20/28)

AGTCACGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGC  >  NC_000913/4193561‑4194143                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      aGTCACGGTCATTCATNGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGNGCGATATAAGAGGGGCGTNCTGCCAACGCGACGTc                                                                                                                                                                                                                                                                                              >  1:668054/1‑299 (MQ=255)             tcatCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCg                                                                                                                                                                                                                                                                                 <  1:470663/300‑1 (MQ=255)                  gCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCAt                                                                                                                                                                                                                                                                             >  2:716719/1‑299 (MQ=255)                  gCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCACTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCAt                                                                                                                                                                                                                                                                             >  2:408990/1‑299 (MQ=255)                                         aCTGTGCCGTTGCCAAATGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGc                                                                                                                                                                                                                                                     >  1:752588/1‑300 (MQ=255)                                         aCTGTGCCGTTGCCAAACGCCGGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGAGACTTCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAggt                                                                                                                                                                                                                                                     <  2:363662/300‑2 (MQ=255)                                         aCTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGATGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGc                                                                                                                                                                                                                                                     <  2:363635/300‑1 (MQ=255)                                         aCTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGc                                                                                                                                                                                                                                                     <  2:19297/300‑1 (MQ=255)                                           tgtgCCGTTGCCAAACGCCAGTCTGCGGCGTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATNACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAACTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGcc                                                                                                                                                                                                                                                    <  1:155682/299‑1 (MQ=255)                                                 gTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCcg                                                                                                                                                                                                                                               <  2:170738/298‑1 (MQ=255)                                                                tCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTgaga                                                                                                                                                                                                                              <  1:403297/300‑1 (MQ=255)                                                                          gAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCtt                                                                                                                                                                                                                    >  2:457467/1‑300 (MQ=255)                                                                               aTGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCNCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCaaa                                                                                                                                                                                                               <  1:564708/300‑1 (MQ=255)                                                                                       gacgacGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCAACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCAAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCAAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCt                                                                                                                                                                                                        >  2:780410/1‑299 (MQ=255)                                                                                                                gTTGCTATCACCGCATGCTCGCATGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGTGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCTAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATActcc                                                                                                                                                                                 <  2:482806/297‑4 (MQ=255)                                                                                                                gTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGcc                                                                                                                                                                                 <  1:477009/297‑1 (MQ=255)                                                                                                                gTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGcc                                                                                                                                                                                 <  2:483819/297‑1 (MQ=255)                                                                                                                gTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCACCAAATGGACGACATACGCCt                                                                                                                                                                                >  2:298110/1‑298 (MQ=255)                                                                                                                 ttGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCGGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGcc                                                                                                                                                                                 <  2:511499/296‑1 (MQ=255)                                                                                                                  tGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATGAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATg                                                                                                                                                                            <  1:476546/300‑1 (MQ=255)                                                                                                                     tATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCt                                                                                                                                                                          <  1:441406/299‑1 (MQ=255)                                                                                                                     tATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCGGCCCCAAATGGACGCCATACGCCTGATGCt                                                                                                                                                                          <  1:613500/299‑1 (MQ=255)                                                                                                                                  gcgcgTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCATGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAgccgc                                                                                                                                                            >  1:289501/1‑300 (MQ=255)                                                                                                                                       tGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTa                                                                                                                                                       >  2:448089/1‑300 (MQ=255)                                                                                                                                            gACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGt                                                                                                                                                  <  2:752588/300‑1 (MQ=255)                                                                                                                                                 aTACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATa                                                                                                                                             <  1:45652/300‑1 (MQ=255)                                                                                                                                                                                                           gTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCa                                                                                   >  1:502328/1‑300 (MQ=255)                                                                                                                                                                                                                aGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACctc                                                                               <  1:709010/299‑1 (MQ=255)                                                                                                                                                                                                                  ccccTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCCCCTCTTc                                                                            >  2:754664/1‑300 (MQ=255)                                                                                                                                                                                                                  ccacTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCAAGCGTGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAATGCCCAGCCGCAGGCCTGCNGCGCGGGTGGCATTGAGATCGGTGGCTTGCAAATCTTACTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCAGCCAGTAAGCGTTGATAAACAATCGCGCGCTCTAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGACTTCCACCTCTTc                                                                            >  2:578941/4‑300 (MQ=255)                                                                                                                                                                                                                       gCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGNGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATcgc                                                                       <  1:172558/300‑1 (MQ=255)                                                                                                                                                                                                                       gCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCAAGCGCGATATAAGAGGGGCGTGCTGCCAACGCAACGTCGATCTCCATATCGTCATGTGTCGAAACGCCCCGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTAATAAACAATCGCGCGTTCTAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTATTCATcgc                                                                       >  1:184513/1‑300 (MQ=255)                                                                                                                                                                                                                          gTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGcc                                                                    >  1:678567/1‑300 (MQ=255)                                                                                                                                                                                                                            gCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCAAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGACAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGTGGcc                                                                                                                   >  2:49109/1‑251 (MQ=255)                                                                                                                                                                                                                                                ttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCNAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGgag                                              <  2:612542/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGTGCATGCTGCCAACGCGACGTCGATTTCCCTATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGagag                                            <  2:172633/300‑1 (MQ=255)                                                                                                                                                                                                                                                     tCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtac                                         <  1:408990/300‑1 (MQ=255)                                                                                                                                                                                                                                                     tCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCAAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtac                                         <  1:230926/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aaCACATGTCCCAGCGCGATATAAGATGGGCGTGCTGACAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGtacgtacg                                    <  2:652197/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 gttccAGTGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGACTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGcc                                  <  2:230190/292‑1 (MQ=255)                                                                                                                                                                                                                                                                 gTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCCGGAGGGTACGTACGCCTGCAt                             >  2:173783/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCAAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACAACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATACGTAGCTGGAGAGTACGTACGCCTGCAt                             >  2:172586/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            atatAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGt                  >  2:157464/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            atatAAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGt                  >  1:36296/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               taAGAGGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTc                >  1:377891/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                aaGATGGGCGTGCTGCCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCAATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGa              <  2:119288/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             tgcCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGc  <  1:181537/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                             tgcCAACGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGc  <  2:24535/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      AGTCACGGTCATTCATCGCCAACTCCTGCAATTTCCAGCAACTGTGCCGTTGCCAAACGCCAGTCTGCGGCTTGAGTAATGGCGCTGACGACGGCGATACTGCCGACACCCGTTGCTATCACCGCAGGCGCGCGTGCCAGACTGATACCGCCAATCGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGC  >  NC_000913/4193561‑4194143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑