Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,746,493	G→A	G134S (GGT→AGT)	yiaO →	2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,746,493	0	G	A	100.0%	242.2 / NA	69	G134S (GGT→AGT)	yiaO	2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/34);  total (35/34)

AACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTCAGCAAAATTTTTCGAAGTGCAGAAGTTCCTTTCTCTGACGCAC  >  NC_000913/3746212‑3746783                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                     aaCATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTAc                                                                                                                                                                                                                                                                                    <  1:481712/300‑1 (MQ=255)           ggcgAAAAAATTCAATGATTTATTGCAGTAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAATTAAAGGACTGAAAGTACTGGCCTACTg                                                                                                                                                                                                                                                                          <  2:413101/300‑1 (MQ=255)           ggcgAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAATGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAANTGTCCGGCTCGAATAACTTTGCCGGGTTATCACCATTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTg                                                                                                                                                                                                                                                                          <  2:237222/300‑1 (MQ=255)                           gATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCAACAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACAGCTGgcgc                                                                                                                                                                                                                                                          >  2:50652/1‑300 (MQ=255)                            aTTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGgcgc                                                                                                                                                                                                                                                          >  1:80965/1‑299 (MQ=255)                               tATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATg                                                                                                                                                                                                                                                      <  1:76135/300‑1 (MQ=255)                                 ttGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTNGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTc                                                                                                                                                                                                                                                    >  1:561293/1‑300 (MQ=255)                                                 aaGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGGGCGATGTCACCAACTCGCGCGCAc                                                                                                                                                                                                                                    <  1:771542/300‑1 (MQ=255)                                                 aaGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTAGCGCGCAc                                                                                                                                                                                                                                    >  2:449678/1‑300 (MQ=255)                                                           gAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAAc                                                                                                                                                                                                                          <  1:87320/300‑1 (MQ=255)                                                            aaaTTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAAcc                                                                                                                                                                                                                         <  1:636817/300‑1 (MQ=255)                                                             aaTTAAAACTGTTCCCGGACAGCACTCTCGGTAACGTGCATGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTGGCCTGGTTATCACCAGTGATGAACTTGTTTGATGTCCCTTTCCTGTTCCGCGATACCACTCACGCGCAGAAAACGCTCGACTGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTTGGAAAACGGCTGGCGCGATGGCACCAACTCGCGCGCACCGGTTAAAAcc                                                                                                                                                                                                                         <  2:109115/299‑1 (MQ=255)                                                                     cTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCAACAGTGATGAACTTGCTTGATGTCCATTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGACAGACTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGTGCGATGTCACCAACTCGCGCGCACCGGTTAAAAccc                                                                                                                                                                                                                        >  2:333132/1‑292 (MQ=255)                                                                       gTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCATTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCt                                                                                                                                                                                                             >  1:323422/1‑300 (MQ=255)                                                                       gTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCATTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAAACCCCG‑CCGACCt                                                                                                                                                                                                             >  1:322951/1‑300 (MQ=255)                                                                                   cACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGaaa                                                                                                                                                                                                         <  2:22146/292‑1 (MQ=255)                                                                                      tctcGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTNTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGaa‑aa                                                                                                                                                                                              <  1:150365/299‑1 (MQ=255)                                                                                       ctcGGTCACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCAACAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGaa‑actc                                                                                                                                                                                            >  1:521063/1‑297 (MQ=255)                                                                                         cGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATcc                                                                                                                                                                                           >  1:6313/1‑299 (MQ=255)                                                                                           gTAACGCGCAGGCGATTATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGta‑aa                                                                                                                                                                                              >  1:520285/1‑294 (MQ=255)                                                                                           gTAACGCGCAGGCGATTATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGta‑aa                                                                                                                                                                                              <  2:520285/294‑1 (MQ=255)                                                                                           gTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCa                                                                                                                                                                                        >  2:756775/1‑300 (MQ=255)                                                                                                     ggCGATGATCAGCGGCNTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAGACCCCCGNCCGACCTGAAAGGGCTGANGAATCCGCACcaggcaa                                                                                                                                                                                >  1:656424/1‑295 (MQ=255)                                                                                                     ggCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATa                                                                                                                                                                               >  1:714585/1‑299 (MQ=255)                                                                                                        gatgatCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATAACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGTTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGcc                                                                                                                                                                            <  1:212582/299‑1 (MQ=255)                                                                                                         atgatCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGccc                                                                                                                                                                           >  1:730225/1‑299 (MQ=255)                                                                                                                          tGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGAGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCAtt                                                                                                                                                         >  1:592550/1‑300 (MQ=255)                                                                                                                                              aaaTGTCCGGCTCGAATAACTTTGCCGGGTTATCACCATTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGTTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAAcc                                                                                                                                     <  2:469310/300‑1 (MQ=255)                                                                                                                                                       gCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCACTCACGCGCATAAAACGCTCGACGGCAAAGTCGTTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGTGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAAcccgatccc                                                                                                                              <  2:80965/298‑1 (MQ=255)                                                                                                                                                           gAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGGTACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCACCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTAGCGCGCACAGGTTAACACCCCCG‑AAGACCTGAAAGGGCTGAA‑CATCCGCACCAACAATAGCCCGATGAATATCGACGCATTAGAAGTCTTTGGCGCTAAc                                                                                                                                      >  2:325952/1‑286 (MQ=255)                                                                                                                                                           gAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGcc                                                                                                                        >  2:325506/1‑300 (MQ=255)                                                                                                                                                                        tggTTATCACCAGTGATGAACTTGCTTGATTTCCCTTTCCTGTGCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTTGCCTACTGGGAAAACGGCTTGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCATTTGCCGAAGTc                                                                                                           <  2:8573/299‑1 (MQ=255)                                                                                                                                                                          gTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCNTCACTTGAAAGTAAAGGACTGAAAGTACTGGCNTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCAGAAGTCta                                                                                                         >  2:594738/1‑300 (MQ=255)                                                                                                                                                                          gTTATCAACAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCta                                                                                                         >  1:427816/1‑300 (MQ=255)                                                                                                                                                                             aTCACCATTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  1:410568/300‑1 (MQ=255)                                                                                                                                                                             aTCACCATTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACACTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  2:401744/300‑1 (MQ=255)                                                                                                                                                                             aTCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAANGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  1:145438/300‑1 (MQ=255)                                                                                                                                                                             aTCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAANGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGAGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  1:146012/300‑1 (MQ=255)                                                                                                                                                                             aTCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGTTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCTCG‑CCGACCTGAAAGGGCTGAA‑AATCCACACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  1:484488/300‑1 (MQ=255)                                                                                                                                                                             aTCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTTGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGTTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  1:227866/300‑1 (MQ=255)                                                                                                                                                                             aTCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  1:228044/300‑1 (MQ=255)                                                                                                                                                                             aTCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  2:402722/300‑1 (MQ=255)                                                                                                                                                                             aTCACCAGCTATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACTGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTTGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCTGATGCCGTTTGCCGAAGTCTATAc                                                                                                      <  2:409593/300‑1 (MQ=255)                                                                                                                                                                              tCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATAcc                                                                                                     <  1:476927/300‑1 (MQ=255)                                                                                                                                                                                   aGTGATGAACTTGCTTGATGTCCCTTTCCTTTTCCGCGATACCGCTCACGCACATAAAACGCGCTACGGCAAAGGCGTTGATGATCTGAANGCCGCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGTACCAACAATAGCCCGATGAATATCGCCGCATGCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTGGCCGAAGTCTATACCGGGCt                                                                                                <  2:614667/300‑1 (MQ=255)                                                                                                                                                                                               gcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGCTCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGTAAACCCGCAc                                                                                    >  1:199588/1‑300 (MQ=255)                                                                                                                                                                                                      gTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGAc                                                                             >  1:741921/1‑300 (MQ=255)                                                                                                                                                                                                      gTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCAGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGAc                                                                             >  1:296295/1‑300 (MQ=255)                                                                                                                                                                                                        cccTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGANTATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGAc                                                                             >  2:621667/1‑298 (MQ=255)                                                                                                                                                                                                            ttCCTGTTCCGCGATANCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTNTATACCGGGCTGGAAACCNGCACTATCGACGCTCa                                                                        >  1:660131/1‑299 (MQ=255)                                                                                                                                                                                                                tGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGaa                                                                    <  1:626383/299‑1 (MQ=255)                                                                                                                                                                                                                tGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAac                                                                   <  1:333132/300‑1 (MQ=255)                                                                                                                                                                                                                  ttCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAacac                                                                 >  2:536019/1‑300 (MQ=255)                                                                                                                                                                                                                  ttCCGCGATACCGCTCAAGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCAATTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCAACAACTAGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAacac                                                                 >  2:647033/1‑300 (MQ=255)                                                                                                                                                                                                                    ccGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGNCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACAccc                                                               >  1:561163/1‑300 (MQ=255)                                                                                                                                                                                                                    ccGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACAccc                                                               >  1:547825/1‑300 (MQ=255)                                                                                                                                                                                                                         aTACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCa                                                          >  1:317474/1‑300 (MQ=255)                                                                                                                                                                                                                         aTACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCa                                                          >  2:652257/1‑300 (MQ=255)                                                                                                                                                                                                                            ccGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAAc                                                        >  1:551928/1‑299 (MQ=255)                                                                                                                                                                                                                              gCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTTGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAAcgt                                                      <  2:342499/299‑1 (MQ=255)                                                                                                                                                                                                                              gCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAAcgt                                                      <  2:714585/299‑1 (MQ=255)                                                                                                                                                                                                                                  aCGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCt                                                 <  1:733774/300‑1 (MQ=255)                                                                                                                                                                                                                                      gcATAAAACGCTCTACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCANCAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTc                                             <  2:561293/300‑1 (MQ=255)                                                                                                                                                                                                                                       cATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTCa                                            >  2:730590/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gTCGGTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACT‑AAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTCAGCAAAATTTTTCGAAGTGCAGa                      >  1:518619/1‑300 (MQ=255)                                                                                                                                                                                                                                                               ggTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTCAGCAAAATTTTTCGAAGTGCAGAAg                    <  1:36311/300‑1 (MQ=255)                                                                                                                                                                                                                                                                gTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTCAGCAAAATTTTTCGAGGTGCAGaaat                   >  2:7015/1‑298 (MQ=255)                                                                                                                                                                                                                                                                gTGATGATCTGAAAGCCTCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTCAGCAAAATTTTTCGAAGTGCAGAAGt                   >  2:705339/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 tCACTTGAAAGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTCAGCAAAATTTTTCGAAGTGCAGAAGTTCCTTTCTCTGACGcac  <  1:50652/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                     AACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGAGAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGTGGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCG‑CCGACCTGAAAGGGCTGAA‑AATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCGATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCGTCTGGTCAGCAAAATTTTTCGAAGTGCAGAAGTTCCTTTCTCTGACGCAC  >  NC_000913/3746212‑3746783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑