Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,359,937	G→A	G247D (GGC→GAC)	gltD →	glutamate synthase subunit GltD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,359,937	0	G	A	100.0%	201.7 / NA	62	G247D (GGC→GAC)	gltD	glutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/36);  total (26/36)

ATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTC  >  NC_000913/3359648‑3360229                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                     aTTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGaa                                                                                                                                                                                                                                                                                            <  2:654884/300‑1 (MQ=255) aTTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGaa                                                                                                                                                                                                                                                                                            <  1:372141/300‑1 (MQ=255)      gcgcgcAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGa                                                                                                                                                                                                                                                                                       >  1:414088/3‑300 (MQ=255)                     aGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCg                                                                                                                                                                                                                                                                         <  1:739357/299‑1 (MQ=255)                      ggTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCgt                                                                                                                                                                                                                                                                        >  1:597857/1‑299 (MQ=255)                      ggTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCgt                                                                                                                                                                                                                                                                        >  2:373003/1‑299 (MQ=255)                          tGGCGTGTGCGGATGGCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGTCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTNAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGATTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTAc                                                                                                                                                                                                                                                                    <  2:565453/299‑1 (MQ=255)                           ggCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGc                                                                                                                                                                                                                                                                  <  2:740146/300‑1 (MQ=255)                              gtgtGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTAGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAg                                                                                                                                                                                                                                                                <  1:309129/299‑1 (MQ=255)                                     gATGTCCTGACGCGTAAAGGCGTAAAAGCAGTTGTCTTCGAACGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTAAAGCTGGGAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAACTCAAACTCAATACCGAAGTGGGCCGCAACGTACAGCTGAACGATCTGTTGAGTGATTACGATGCCTTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGCTGGCGTGTACGCAgc                                                                                                                                                                                                                                                               >  2:389224/1‑293 (MQ=255)                                        gTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTc                                                                                                                                                                                                                                                     >  2:750930/1‑300 (MQ=255)                                        gTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTAAACGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTCCAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCAGATGGCGTGTACGCAGCGCTGCCGTTc                                                                                                                                                                                                                                                     >  2:286824/1‑300 (MQ=255)                                        gTCCTGACGCGTAACGGCGTAAAAGCCGTTGTATTCGAACGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCAGTGAAATCTTCAACGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGAAGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGACGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGCGTACGccaagctaccgttc                                                                                                                                                                                                                                                     >  2:286925/1‑287 (MQ=255)                                                        gCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCa                                                                                                                                                                                                                                     >  2:202395/1‑300 (MQ=255)                                                                   gTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTaagg                                                                                                                                                                                                                          <  1:764333/300‑3 (MQ=255)                                                                      gTCTTCGACCGTCATCCAGAAATTGGCGTGCTGCTGACCTTCGTTATTCCGGCCTTCAAGCGGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCTTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGc                                                                                                                                                                                                                       <  1:480667/300‑1 (MQ=255)                                                                      gTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGc                                                                                                                                                                                                                       >  1:609143/1‑300 (MQ=255)                                                                      gTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATCCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGc                                                                                                                                                                                                                       >  1:609218/1‑300 (MQ=255)                                                                             aCCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTGCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTg                                                                                                                                                                                                                <  2:635775/300‑1 (MQ=255)                                                                                   aTCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCTGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAAcc                                                                                                                                                                                                           <  1:420052/299‑1 (MQ=255)                                                                                   aTCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAAcc                                                                                                                                                                                                           <  1:771415/299‑1 (MQ=255)                                                                                    tCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGAGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAAccc                                                                                                                                                                                                          >  2:371970/1‑299 (MQ=255)                                                                                                    ctgctgACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGNTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACAACAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGt                                                                                                                                                                                          >  2:572360/1‑299 (MQ=255)                                                                                                    ctgctgACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTc                                                                                                                                                                                         >  1:459813/1‑300 (MQ=255)                                                                                                      gctgACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGTTAATTACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACTGTTCGTCAg                                                                                                                                                                                       <  2:448906/300‑1 (MQ=255)                                                                                                                 gTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGNTGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCa                                                                                                                                                                            >  2:565357/1‑300 (MQ=255)                                                                                                                   aTTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCaaa                                                                                                                                                                          <  1:425436/300‑1 (MQ=255)                                                                                                                                     gAAAAAGAGGTAATGACGCGTCGCCGGGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGTGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGc                                                                                                                                                        <  2:130648/300‑1 (MQ=255)                                                                                                                                              gTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGac                                                                                                                                               >  1:196582/1‑300 (MQ=255)                                                                                                                                                           gCCGTTAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGTTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCGCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                                   <  2:37087/299‑1 (MQ=255)                                                                                                                                                           gCCGTGAAATCTTCAGCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                                   <  1:417690/299‑1 (MQ=255)                                                                                                                                                           gCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                                   <  1:79427/299‑1 (MQ=255)                                                                                                                                                           gCCGTGAAATCTTCACCGGCATGGGTATTGAATGCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                                   <  1:755830/299‑1 (MQ=255)                                                                                                                                                                gAAATCTTCACCTGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                                                                                                                                   <  1:215932/294‑1 (MQ=255)                                                                                                                                                                    tCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCANCGCTGCCGTTCCTCATCGCCAACACCAAACAGNTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTa                                                                                                                         <  2:597857/300‑1 (MQ=255)                                                                                                                                                                    tCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTa                                                                                                                         <  1:264769/300‑1 (MQ=255)                                                                                                                                                                         aCCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGGGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTc                                                                                                                     <  2:473731/299‑1 (MQ=255)                                                                                                                                                                            tgCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCg                                                                                                                   <  1:334344/297‑1 (MQ=255)                                                                                                                                                                            ggCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCg                                                                                                                   <  1:333757/298‑1 (MQ=255)                                                                                                                                                                            ggCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCAGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAAAGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCACCAACAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGt                                                                                                                  >  1:214325/1‑299 (MQ=255)                                                                                                                                                                                 gggTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTACTGGGCTTTGGTGACACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCTTGGTGGTCCTTGTCGGTGGCGACCCTGCGATGGACTGCGTGCGTACGTCCGTGCGCCa                                                                                                            >  2:376406/1‑300 (MQ=255)                                                                                                                                                                                   gTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCa                                                                                                            >  1:108043/1‑298 (MQ=255)                                                                                                                                                                                    tATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAggg                                                                                                         <  1:221825/300‑1 (MQ=255)                                                                                                                                                                                       tGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGc                                                                                                      <  1:306252/300‑1 (MQ=255)                                                                                                                                                                                                     aTACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCt                                                                                        >  2:717209/1‑300 (MQ=255)                                                                                                                                                                                                                    gcgACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATg                                                                                                                                                                                                                          <  1:446377/155‑1 (MQ=255)                                                                                                                                                                                                                    gcgACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATg                                                                                                                                                                                                                          >  2:446377/1‑155 (MQ=255)                                                                                                                                                                                                                      gACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGtgat                                                                       <  2:196582/300‑1 (MQ=255)                                                                                                                                                                                                                       aCGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCANCACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGtgat                                                                       >  2:563414/1‑299 (MQ=255)                                                                                                                                                                                                                       aCGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACAACAAACAGTTAATGGGCTTTGGTGAAAACCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGtgat                                                                       >  2:5510/1‑299 (MQ=255)                                                                                                                                                                                                                          tACAGCTGGACGATCTGCTGAGTGATTACGATGCCGGGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAag                                                                   <  1:285095/300‑1 (MQ=255)                                                                                                                                                                                                                              gCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGaa                                                               >  2:767553/1‑300 (MQ=255)                                                                                                                                                                                                                                          gctgAGTGATTACGATGCCGTGTTCCTTGGCGTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTc                                                   >  2:105627/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGgaagaa                    >  2:469213/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGgaagaa                    <  1:50136/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gTCGAGACTTATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCAACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGgaagaa                    <  1:49856/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            gagACTTATCAGGCAATGCGCGACGGGCGGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAAGAGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAgg                  <  2:735237/297‑1 (MQ=255)                                                                                                                                                                                                                                                                             agacgtATCAGGCAATGCGCGACGGGCTGGAAAACTAAGACGCCGATTGCGTGTACGCAGCGCTGCCGTTCCGCATCGCCAACACCAAACAGTTAATGGTCTTTGGTGAAACCCGCGACGAACCGTTCTTCAGCATGGAAGGCAAACGCGTGTTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCTAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCTCGAAGTGAAAAACGCGCGGGAAGAAgg                  <  2:374803/293‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ttATCAGTCAATGCGCNACGGGCTGGAAAACGAAGNCGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCNCGGGAAGAAGGCGTag             <  1:667925/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ttATCAGTCAATGCGCGACGGGCTGGAAAACGAAGACGTCGATGGCGTGTACGCAGCGCGGCCGTTCCTCATCGCCAACACCAAACAGTTAATCGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTTGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGCGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTTAAAAACGCGCGGGAAGAAGGCGTag             <  1:284095/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tATCAGTCAATGCGCGAAGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGGTAATGGGCTTGGGTGAAAACCGCGACGAACCATTCGTCAGCATGGAAGGCAAACGCGTGGGGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCTTCTTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAATAAGGCGTagag           <  2:160645/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           aTGCGCGACGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTc  >  1:644438/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                     ATTATCGGCGCAGGCCCGGCAGGTCTGGCGTGTGCGGATGTCCTGACGCGTAACGGCGTAAAAGCCGTTGTCTTCGACCGTCATCCAGAAATTGGCGGGCTGCTGACCTTCGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGTCGCCGTGAAATCTTCACCGGCATGGGTATTGAATTCAAACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTC  >  NC_000913/3359648‑3360229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑