breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 70,659 G→A W91* (TGG→TGA araC → DNA‑binding transcriptional dual regulator AraC
RA 83,882 G→A intergenic (‑174/‑486) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
RA 100,946 T→C M61T (ATG→ACG)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 244,775 G→A G150D (GGC→GAC)  yafJ → putative glutamine amidotransferase YafJ
RA 245,662 G→A I48I (ATC→ATT dpaA ← peptidoglycan meso‑diaminopimelic acid protein amidase A
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
RA 248,203 G→A intergenic (+69/‑2695) rayT → / → dinB REP‑associated tyrosine transposase/DNA polymerase IV
RA 250,009 G→A intergenic (+1875/‑889) rayT → / → dinB REP‑associated tyrosine transposase/DNA polymerase IV
RA 251,629 C→T G244G (GGC→GGT dinB → DNA polymerase IV
MC JC 256,108 Δ115,274 bp [gpt][mhpC] 117 genes
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
JC JC 607,765 IS150 (+) +3 bp noncoding (26‑28/60 nt) sokE ← small RNA SokE
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,016,006 G→A I155I (ATC→ATT fabA ← beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase
JC JC 1,051,342 IS5 (+) +4 bp intergenic (‑167/‑116) cspH ← / → cspG CspA family protein CspH/cold shock protein CspG
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,069,743 C→T A33T (GCA→ACA)  rutG ← pyrimidine:H(+) symporter
RA 1,093,346 (T)6→5 coding (471/1359 nt) dgcT → putative diguanylate cyclase DgcT
RA 1,098,620 T→A V245V (GTT→GTA ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,196,325 A→G K401K (AAA→AAG icd → isocitrate dehydrogenase
JC JC 1,291,105 IS1 (–) +9 bp coding (864‑872/1014 nt) rssB → regulator of RpoS
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,398,925 C→T G201D (GGT→GAT)  fnr ← DNA‑binding transcriptional dual regulator FNR
MC JC 1,411,925 Δ23,060 bp [ttcA]ynaM 34 genes
MC JC 1,506,416 Δ45 bp coding (245‑289/537 nt) sutR → DNA‑binding transcriptional dual regulator SutR
RA 1,539,947 C→G A882P (GCG→CCG)  narZ ← nitrate reductase Z subunit alpha
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,680,548 C→T S331F (TCC→TTC)  ydgI → putative arginine:ornithine antiporter
RA 1,728,754 C→T A136A (GCC→GCT rnt → ribonuclease T
RA 1,829,887 G→A A293A (GCC→GCT astA ← arginine N‑succinyltransferase
RA 1,855,402 C→T A190A (GCG→GCA ydjG ← NADH‑dependent methylglyoxal reductase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,042,171 G→A intergenic (+146/‑197) zinT → / → yodB metal‑binding protein ZinT/putative cytochrome b561 YodB
RA 2,044,642 T→C intergenic (+18/‑296) asnT → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,325,037 C→T P94P (CCC→CCT atoE → short chain fatty acid transporter
RA 2,339,161 T→C D87G (GAC→GGC)  gyrA ← DNA gyrase subunit A
JC JC 2,468,298 IS3 (+) +3 bp coding (85‑87/921 nt) yfdH → bactoprenol glucosyl transferase
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,744,030 T→C Y136H (TAT→CAT)  yfiB → lipoprotein YfiB
JC JC 2,819,278 IS5 (+) +4 bp intergenic (‑132/+100) csrA ← / ← alaS carbon storage regulator/alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,866,609 C→A E315* (GAA→TAA)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,867,454 2 bp→AA coding (97‑98/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,939,297 G→T G137V (GGG→GTG)  fucU → L‑fucose mutarotase
RA 3,131,139 T→A L309Q (CTG→CAG)  insH9 → IS5 family transposase and trans‑activator
RA 3,152,494 G→T A87S (GCG→TCG)  metC → cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,258,484 T→C Q389R (CAA→CGA)  tdcG ← L‑serine deaminase III
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
JC JC 3,350,556 IS1 (–) +8 bp intergenic (‑97/‑14) elbB ← / → arcZ low activity glyoxalase ElbB/small regulatory RNA ArcZ
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,444,905 C→T G109S (GGT→AGT)  rpsE ← 30S ribosomal subunit protein S5
RA 3,465,502 G→A E141K (GAG→AAG)  gspM → Type II secretion system protein GspM
RA 3,474,424 T→G K43N (AAA→AAC rpsL ← 30S ribosomal subunit protein S12
MC JC 3,485,658 Δ5,170 bp [yhfA][pabA] [yhfA], crp, yhfK, argD, yrfJ, [pabA]
RA 3,511,362 C→T G21S (GGC→AGC)  yhfY ← PRD domain‑containing protein YhfY
RA 3,560,443 C→G intergenic (+577/+179) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
MC JC 3,653,231 Δ10,571 bp IS5‑mediated yhiSmdtF yhiS, slp, dctR, yhiD, hdeB, hdeA, hdeD, arrS, gadE, gadF, mdtE, mdtF
RA 3,707,878 A→G intergenic (‑173/+738) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,707,883 G→A intergenic (‑178/+733) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,815,264 C→T E169K (GAA→AAA)  pyrE ← orotate phosphoribosyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC JC 3,888,869 IS1 (+) +9 bp coding (140‑148/1416 nt) tnaA → tryptophanase
RA 3,979,080 C→T R160R (CGC→CGT wzyE → enterobacterial common antigen polymerase
RA 3,979,723 C→T P375S (CCT→TCT)  wzyE → enterobacterial common antigen polymerase
RA 4,043,852 G→A W145* (TGG→TGA dsbA → thiol:disulfide oxidoreductase DsbA
RA 4,043,901 G→A A162T (GCT→ACT)  dsbA → thiol:disulfide oxidoreductase DsbA
RA 4,046,594 G→A N3N (AAC→AAT yihG ← 1‑acylglycerol‑3‑phosphate O‑acyltransferase YihG
RA 4,047,415 G→A T150T (ACG→ACA polA → DNA polymerase I
RA 4,051,809 G→A E155K (GAA→AAA)  yihI → Der GTPase‑activating protein YihI
RA 4,054,219 G→A P354S (CCA→TCA)  glnG ← DNA‑binding transcriptional dual regulator NtrC
RA 4,061,021 G→A S192N (AGC→AAC)  yihL → putative DNA‑binding transcriptional regulator YihL
RA 4,065,117 G→A T217M (ACG→ATG)  yihO ← putative sulfoquinovose transporter
RA 4,073,952 G→A A72T (GCA→ACA)  yihV → 6‑deoxy‑6‑sulfofructose kinase
RA 4,080,078 G→A intergenic (+109/+221) yiiF → / ← fdhE protein YiiF/formate dehydrogenase formation protein
RA 4,097,875 T→C I47T (ATT→ACT)  rhaS → DNA‑binding transcriptional activator RhaS
RA 4,098,150 G→A D139N (GAT→AAT)  rhaS → DNA‑binding transcriptional activator RhaS
RA 4,100,227 G→A S100L (TCG→TTG)  rhaT ← rhamnose/lyxose:H(+) symporter
RA 4,101,785 G→A A32A (GCG→GCA kdgT → 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
RA 4,105,917 G→A G33D (GGT→GAT)  cpxP → periplasmic protein CpxP
RA 4,117,807 G→A A95V (GCC→GTC)  glpF ← glycerol facilitator
RA 4,122,023 G→A L89L (CTG→TTG)  hslV ← peptidase component of the HslVU protease
RA 4,123,470 G→T C329* (TGC→TGA cytR ← DNA‑binding transcriptional repressor CytR
RA 4,124,375 G→A R28* (CGA→TGA)  cytR ← DNA‑binding transcriptional repressor CytR
RA 4,128,813 Δ2 bp coding (142‑143/1161 nt) metB → O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
RA 4,137,772 G→A intergenic (‑115/+160) yijF ← / ← gldA DUF1287 domain‑containing protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA 4,153,441 G→A intergenic (‑343/+255) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,154,691 G→A Q53* (CAG→TAG)  argE ← acetylornithine deacetylase
RA 4,182,782 A→T Q513L (CAG→CTG)  rpoB → RNA polymerase subunit beta
RA 4,189,636 G→A intergenic (+63/‑150) rpoC → / → yjaZ RNA polymerase subunit beta'/protein YjaZ
RA 4,192,667 G→A I57I (ATC→ATT thiS ← sulfur carrier protein ThiS
RA 4,204,555 G→A A412A (GCG→GCA zraR → DNA‑binding transcriptional activator ZraR
RA 4,212,973 Δ1 bp intergenic (+27/‑67) rrlE → / → rrfE 23S ribosomal RNA/5S ribosomal RNA
RA 4,220,685 A→G F601F (TTT→TTC arpA ← regulator of acetyl CoA synthetase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,264,192 C→T E14K (GAA→AAA)  qorA ← putative quinone oxidoreductase 1
RA 4,287,464 T→A intergenic (‑93/‑147) acs ← / → ytcB acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,405,043 G→A E119E (GAG→GAA purA → adenylosuccinate synthetase
RA 4,415,505 C→T L411L (CTG→TTG)  aidB → putative acyl‑CoA dehydrogenase AidB
RA 4,418,060 C→T D132N (GAT→AAT)  ulaR ← DNA‑binding transcriptional repressor UlaR
RA 4,423,110 G→A D89N (GAC→AAC)  ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE
RA 4,444,187 G→A V26M (GTG→ATG)  tamB → translocation and assembly module subunit TamB
RA 4,455,812 G→A G10S (GGC→AGC)  mpl → UDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
RA 4,457,427 G→A Q147* (CAA→TAA)  yjgA ← putative ribosome biogenesis factor YjgA
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
JC JC 4,542,250 IS1 (+) +9 bp coding (214‑222/597 nt) fimE → regulator for fimA
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196352 1211461 15110 7 [5] [6] 7 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1292514 1292677 164 7 [5] [6] 7 hns DNA‑binding transcriptional dual regulator H‑NS
* * ÷ NC_000913 2172548 2175524 2977 33 [0] [0] 37 [gatD]–[gatZ] [gatD],gatB,gatA,[gatZ]
* * ÷ NC_000913 4035390–4035733 4036886–4035736 4–1497 7 [6] [5] 7 [rrsA] [rrsA]
* * ÷ NC_000913 4088916 4088995 80 7 [6] [6] 7 frvR putative DNA‑binding transcriptional regulator FrvR
* * ÷ NC_000913 4166485–4168220 4168509 290–2025 7 [6] [6] 8 rrsB–gltT rrsB,gltT

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)20 (0.540) 20/482 0.9 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1978503 =NA (NA)37 (0.980) 34/490 0.2 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 2175525 = 0 (0.000)coding (797/1263 nt) gatZ putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
* ? NC_000913 = 1979270NA (NA)32 (0.850) 29/490 0.4 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 2172547 0 (0.000)coding (327/1041 nt) gatD galactitol‑1‑phosphate 5‑dehydrogenase
* ? NC_000913 2818197 =32 (0.850)17 (0.660) 16/332 0.6 46.1% intergenic (‑62/+1) argZ/argY tRNA‑Arg/tRNA‑Arg
?NC_000913 = 2818471 18 (0.700)intergenic (‑197/+2) argY/argV tRNA‑Arg/tRNA‑Arg
* ? NC_000913 3013860 =9 (0.240)28 (0.750) 27/486 0.4 75.7% coding (380/1626 nt) yqeB XdhC‑CoxI family protein YqeB
?NC_000913 3720633 = NA (NA)noncoding (1/1443 nt) IS150 repeat region
* ? NC_000913 = 30138629 (0.240)31 (0.820) 30/488 0.3 77.6% coding (378/1626 nt) yqeB XdhC‑CoxI family protein YqeB
?NC_000913 = 3722075 NA (NA)noncoding (1443/1443 nt) IS150 repeat region
* ? NC_000913 4542682 =9 (0.240)13 (0.360) 13/472 1.6 59.5% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 9 (0.250)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 45426908 (0.210)7 (0.190) 7/472 2.7 45.6% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 9 (0.250)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit