Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,204,555	G→A	A412A (GCG→GCA)	zraR →	DNA‑binding transcriptional activator ZraR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,204,555	0	G	A	100.0%	154.4 / NA	44	A412A (GCG→GCA)	zraR	DNA‑binding transcriptional activator ZraR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

GCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTACGCGCC  >  NC_000913/4204310‑4204787                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         gCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCt                                                                                                                                                                                                                                     <  1:259744/251‑1 (MQ=255)   cGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGGGGAAGGGGAAAAAGAGGTGATTCTGGCAGCGCTgg                                                                                                                                                                                                                                   >  2:359697/1‑251 (MQ=255)    gAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTgg                                                                                                                                                                                                                                   >  1:55710/1‑250 (MQ=255)      gCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGTGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGgaga                                                                                                                                                                                                                                <  1:238500/251‑1 (MQ=255)        gTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATAGTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGaaa                                                                                                                                                                                                                              <  2:230875/251‑1 (MQ=255)               cAAGGCTGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACTCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGTATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCTCTGTAGAAAACGggcg                                                                                                                                                                                                                       <  1:130229/251‑1 (MQ=255)                    cGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGcaac                                                                                                                                                                                                                  >  1:212813/1‑251 (MQ=255)                      gTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGcaacaa                                                                                                                                                                                                                <  1:321561/251‑1 (MQ=255)                      gTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGcaacaa                                                                                                                                                                                                                <  1:87844/251‑1 (MQ=255)                          aaGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAAcc                                                                                                                                                                                                            >  2:205064/1‑251 (MQ=255)                               tttACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAAGATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAg                                                                                                                                                                                                        <  2:112297/250‑1 (MQ=255)                                 tACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAgcc                                                                                                                                                                                                      <  1:16888/250‑1 (MQ=255)                                   cGCCCCAGGTGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGGGATTCTTGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAgccgcc                                                                                                                                                                                                   <  1:170985/251‑1 (MQ=255)                                         aGGCGATGGATCTGTTGATTCATTACGACGGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAg                                                                                                                                                                                             <  2:97326/251‑1 (MQ=255)                                           gCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGtt                                                                                                                                                                                           <  2:29638/251‑1 (MQ=255)                                             gATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGtt                                                                                                                                                                                           >  1:272724/1‑249 (MQ=255)                                                      gttgaatcactaagacacgcggggAAATATTCGCGCGCTGGTAAACGCGGTGGAACGGGCAGTGGAGCAGCTGACCGGGGCATATCTTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGAAACAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATcccgg                                                                                                                                                                                >  2:215130/22‑247 (MQ=255)                                                      gTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCGGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGAt                                                                                                                                                                                     <  2:275266/246‑1 (MQ=255)                                                         gATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCa                                                                                                                                                                             <  2:3057/251‑1 (MQ=255)                                                            tCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCaaa                                                                                                                                                                           >  1:19123/1‑250 (MQ=255)                                                                         ccGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCaa                                                                                                                                                              >  2:63792/1‑250 (MQ=255)                                                                                     cGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGtt                                                                                                                                                  >  2:286527/1‑250 (MQ=255)                                                                                                         ggAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATa                                                                                                                              <  1:174949/250‑1 (MQ=255)                                                                                                                    gggggtggctgCCCGGGGAATATATTGCCGAACGAGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCTGGATATTCAGCCGTTGGTGGCAGTGGAAAAAGAGGTGATTATGGCAGCGCTGGAGAAAACGGGCGGCAAAAAACCCGCAGCCGCCCTGCAGTGAGGGATCACGCGCAACACGATATTGGGAATACTGTCGCGTTAGTTCGGCTCGCGTACGATAGCGCGCCAGCCg                                                                                                                  <  2:198958/244‑1 (MQ=255)                                                                                                                          tgctgACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCt                                                                                                            >  2:183501/1‑251 (MQ=255)                                                                                                                               aCCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTCTTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCg                                                                                                       >  2:168734/1‑251 (MQ=255)                                                                                                                                ccGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGGACGCCGATCCCGCTGGGACAAAGTGAGGAGATTCAGACGTTGGTGGAAGGGTAAACAGAAGTGATTCTGGCAGCGCTGGATGAAACGGTCGGCAACAAAACCGAAGCCGCCCGTCAGGTAGGGATCACGCGCAAACCGCTATTGGCAAAACTGTCGCATGGGTTCTTCTCGCGTTCGATAACGAGCCAGCCGCTATCGTTcggg                                                                                                      >  2:121349/1‑248 (MQ=255)                                                                                                                                      aaTATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCACAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGacg                                                                                                >  2:132420/1‑249 (MQ=255)                                                                                                                                             ttCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACACAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGCTCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgt                                                                                         >  2:342780/1‑251 (MQ=255)                                                                                                                                                   ccgcgGGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCGGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGt                                                                                   <  2:66284/250‑1 (MQ=255)                                                                                                                                                    cgcgAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTg                                                                                  >  2:83386/1‑251 (MQ=255)                                                                                                                                                                             gtccGCCGAGCCCGCTGGGACAAAGTAAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAAAAAAACCGAAGCCGCCCGTCAGTTAGGTATCACGCGCAAAACGCTATTGGCAAAACTGTCGCTTGAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgcg                                                         <  2:362713/248‑1 (MQ=255)                                                                                                                                                                                   cGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTc                                                   >  2:203924/1‑251 (MQ=255)                                                                                                                                                                                             gggACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAACCGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTcc                                                                                                        >  2:50185/1‑188 (MQ=255)                                                                                                                                                                                             gggACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTcc                                                                                                        <  1:50185/188‑1 (MQ=255)                                                                                                                                                                                               gACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATGGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTGTCCGGCATAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                       <  1:215130/251‑1 (MQ=255)                                                                                                                                                                                                  aaaGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAACCGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACg                                    <  2:255453/251‑1 (MQ=255)                                                                                                                                                                                                          gATATTCAGCCGTTGGTGGAAGCGGAAAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAcc                            >  2:62528/1‑251 (MQ=255)                                                                                                                                                                                                                         gtggaagtggaaAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGc              >  2:242010/1‑250 (MQ=255)                                                                                                                                                                                                                         gtggaagtggaaAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGc              >  1:365055/1‑250 (MQ=255)                                                                                                                                                                                                                           ggaagtggaaAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAACACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGcac            >  2:261783/1‑250 (MQ=255)                                                                                                                                                                                                                             aagtggcaAAAGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGt         >  1:253322/1‑251 (MQ=255)                                                                                                                                                                                                                                 ggaaAAAGAGGTGCTTCTGGCAGCGCTGAAGAAAACGGGCGGCACCAAAACCGAAGCCGCCCGTCAGTGAGGGTTCACGCGCAAAACGCTTTGGGCAAAACTGACGCGTTAGTTCTGCTCGCGTTCGATGGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACACTAcgg     <  2:48561/251‑2 (MQ=255)                                                                                                                                                                                                                                    aaaaGAGGTGATTCTGGCAGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTACGcgcc  >  2:94656/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         GCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTACGCGCC  >  NC_000913/4204310‑4204787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑