Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,642	T→C	intergenic (+18/‑296)	asnT → / → yeeJ	tRNA‑Asn/inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,642	0	T	C	100.0%	90.3 / NA	25	intergenic (+18/‑296)	asnT/yeeJ	tRNA‑Asn/inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/16);  total (9/16)

GACTGCATCACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATT  >  NC_000913/2044396‑2044864                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                               gaCTGCATCACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCtctttt                                                                                                                                                                                                                            <  1:205912/251‑1 (MQ=255)     gCATCACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAgcg                                                                                                                                                                                                                        <  2:325992/251‑1 (MQ=255)                         aCTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGtt                                                                                                                                                                                                    >  2:335585/1‑251 (MQ=255)                            cGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTg                                                                                                                                                                                                   <  1:335585/249‑1 (MQ=255)                               tttCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACAt                                                                                                                                                                                              >  1:356715/1‑251 (MQ=255)                                    gCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttt                                                                                                                                                                                         >  2:280084/1‑251 (MQ=255)                                      gacgaATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttttt                                                                                                                                                                                       >  1:18546/1‑251 (MQ=255)                                              ttCATTAATTAAAAACTTTGCAGATTAATTAACCACTTGAAATGACTTATGCAATTTAGTGTTAACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGa                                                                                                                                                                                >  1:28063/1‑250 (MQ=255)                                                cATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATa                                                                                                                                                                              <  2:126232/250‑1 (MQ=255)                                                               atGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTATTGTTGACAGACAAGGTACCGCTAAATAATATTCGCCCCGTTCACACGAGTCCTCTGTAGTTCAGTCGGTAGAACGGCGTACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAGTTCGCTCTTTTAGCGCAATGTCTCTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACa                                                                                                                                                                <  1:224761/248‑1 (MQ=37)                                                                               cAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCGCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGTCCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAcc                                                                                                                                              <  2:248335/251‑1 (MQ=255)                                                                                     aaaTGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg                                                                                                                                        <  1:28113/251‑1 (MQ=255)                                                                                                             gacagacaAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACg                                                                                                                <  2:74098/251‑1 (MQ=255)                                                                                                                          ccGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCAtttt                                                                                                   <  2:12611/251‑1 (MQ=255)                                                                                                                                aGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTc                                                                                             >  1:203378/1‑251 (MQ=255)                                                                                                                                    atatTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGGCCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACAGCTTAAGCGCCCTCGACCTTGATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGAtt                                                                                         <  1:236613/251‑1 (MQ=255)                                                                                                                                    atatTCGACCCGTTCACACGATTCCGCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGAtt                                                                                         <  1:206430/251‑1 (MQ=255)                                                                                                                                     tatTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGAtt                                                                                         >  1:290013/1‑250 (MQ=255)                                                                                                                                                              ctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGAATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCCTCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTAAGTAGtct                                                               >  1:353274/1‑251 (MQ=255)                                                                                                                                                               tGTAGTTCAGTCGGTAGAACGGCGGACGGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGCGGAGCCAAATTCTAACAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGtctc                                                              >  2:339432/1‑251 (MQ=255)                                                                                                                                                                gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGtc                                                                <  1:162375/248‑1 (MQ=255)                                                                                                                                                                                        aCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCATTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGATGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTTAGTTATGCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAAc                                     <  2:285110/251‑1 (MQ=255)                                                                                                                                                                                             tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGcc                                 <  2:18546/250‑1 (MQ=255)                                                                                                                                                                                                       gTCACTGGTTCGAGGCCAGGCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGGCCTTTATGGTTTAGGGCGTTTTGCTATGAACGGCATCACCATTTTCCCCTCGATTATAAAACTTGAGGTAGGCAGTAGTCTCCCCTCGTGCAACTCACACCCAAAACTGCCTAACGAAAAg                       <  1:350610/250‑1 (MQ=255)                                                                                                                                                                                                             ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGttatta                 <  2:268849/250‑1 (MQ=255)                                                                                                                                                                                                                           cAGAGGAGCCAAATTCTAAAAATTCGCTCTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGAGAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTGTCAATCATAtt  <  2:305372/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                               GACTGCATCACGCTAATGATACAGACTCGTTTTCGGCGACGAATGTTCATTAATTAACAACTTTGCAGATTAATTAACCAATTGAAATGACTTATGAAATTTAGTGTTGACAGACAAGGTACCGCTAAGTAATATTCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGTTATTAATTTTCAATCATATT  >  NC_000913/2044396‑2044864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑