Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	245,662	G→A	I48I (ATC→ATT)	dpaA ←	peptidoglycan meso‑diaminopimelic acid protein amidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	245,662	0	G	A	100.0%	100.1 / NA	29	I48I (ATC→ATT)	dpaA	peptidoglycan meso‑diaminopimelic acid protein amidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

AACCATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAAAG  >  NC_000913/245427‑245906                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     aaCCATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACa                                                                                                                                                                                                                                       <  1:51055/251‑1 (MQ=255)            cGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCAt                                                                                                                                                                                                                            >  2:341230/1‑251 (MQ=255)                               aaCCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTCAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATActct                                                                                                                                                                                                         >  1:296149/1‑251 (MQ=255)                                aCCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCtt                                                                                                                                                                                                        <  1:148360/251‑1 (MQ=255)                                                ttGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTgg                                                                                                                                                                                         <  1:370973/250‑1 (MQ=255)                                                             tgacTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCTGGGCTTTTGAAATCGCCCTGACGCGGCTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTAGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATTTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACATGCGTGGTAGAGCTAatgctg                                                                                                                                                                           >  2:2168/4‑251 (MQ=255)                                                                                aCGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATg                                                                                                                                                        >  2:117042/1‑251 (MQ=255)                                                                                aCGCTGGACGCTATAAAACCCTTCCGGGCTTTGGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATT‑ACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATg                                                                                                                                                        <  1:307003/250‑1 (MQ=255)                                                                                    tGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGACAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAAc                                                                                                                                                    >  2:332516/1‑251 (MQ=255)                                                                                      gACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGc                                                                                                                                                  >  1:110607/1‑251 (MQ=255)                                                                                                 aCCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATcaac                                                                                                                                       >  2:34264/1‑251 (MQ=255)                                                                                                    cTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCaacaac                                                                                                                                    >  2:143492/1‑251 (MQ=255)                                                                                                           gCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCa                                                                                                                                                                       >  2:11027/1‑209 (MQ=255)                                                                                                           gCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCa                                                                                                                                                                       <  1:11027/209‑1 (MQ=255)                                                                                                                                           cTAAGACGCAGGACTATTTACAGATTTTATCGCTGGCGGGCAGTTGATATTGCTCGCCCATTTTGACGTCGCGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTAtt                                                                                             >  2:235303/1‑251 (MQ=255)                                                                                                                                             aaGCCGCCGGAATAGTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCGTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCGGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTACTGCCCAGCAAACCGGCAAATGAAATTCACGGGATTAACAACGGCGCAAGAGTTAATGCGATTTTACGCATACTGCTTATTc                                                                                            <  1:35713/250‑1 (MQ=255)                                                                                                                                                    cgGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATa                                                                                     >  2:311032/1‑250 (MQ=255)                                                                                                                                                                      tataGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGCTCGAGCGTACGTTCTTCCTTGAAAATCTGGATGAAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCACCAACATCGCCAGAATTAATGCGATTTTACGCATACTGCTTCTTCCTTGATAAAACGGTTACACACGcccg                                                                  >  2:82152/1‑249 (MQ=255)                                                                                                                                                                                         atatTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCCTACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATcaca                                               <  2:96893/251‑4 (MQ=255)                                                                                                                                                                                         atatTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCa                                               <  1:189195/251‑1 (MQ=255)                                                                                                                                                                                          tatTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATTCTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCa                                               >  1:325217/1‑250 (MQ=255)                                                                                                                                                                                           atTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCaaa                                             <  1:108226/251‑1 (MQ=255)                                                                                                                                                                                             ggCTCGCCCATTTTGACGGAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACATGCTTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATGCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCaaagc                                           <  2:249426/250‑3 (MQ=255)                                                                                                                                                                                                 cGCCCCTTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTAATGCTGCCCAGCAAACCGGCAAATGAATCGCACGGGATCAACAACATCGCAAGAATTAATTCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTCCACACGCCAGGACGGCAAAATGGATCCAAAATCga                                         <  2:194617/249‑2 (MQ=255)                                                                                                                                                                                                             cGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGAttt                           <  1:341230/251‑1 (MQ=255)                                                                                                                                                                                                                      cGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTc                  >  2:199575/1‑251 (MQ=255)                                                                                                                                                                                                                      cGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTc                  <  1:117042/251‑1 (MQ=255)                                                                                                                                                                                                                          cGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACAt              <  1:124014/251‑1 (MQ=255)                                                                                                                                                                                                                                        tGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAAag  >  1:75034/1‑249 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     AACCATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAAAG  >  NC_000913/245427‑245906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑