Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	274.6 / NA	60	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (30/30);  total (30/30)

GCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACT  >  NC_000913/3207314‑3207797                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                         gctgctGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaa                                                                                                                                                                                                                                           >  1:306822/1‑250 (MQ=255)     ctgGTTCATGACGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACg                                                                                                                                                                                                                                      >  1:325289/1‑251 (MQ=255)          tCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAGCCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGa                                                                                                                                                                                                                                  <  1:339163/250‑1 (MQ=255)            aTGCCGATTACACCTTTGAAAATCTGTCGCTCCCCCGGAGCGCGTTATGAAAAAGATCCTGCCAACCCCGAT‑CAAAGGTGAAGTGCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑CCCTGCCGCGTCGTTTGGCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGGAAAGCGATTTTCCACGCTG‑CCTCATCGTGCGAAAAAACGGCGACaa                                                                                                                                                                                                                               <  2:190015/251‑1 (MQ=255)              gCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAAt                                                                                                                                                                                                                             >  2:311008/1‑251 (MQ=255)                  atcgctccTTTGAAAATCTGCCTCACACCCGGAGCTGGTTCTGAAAAAGATCCTGA‑AACCCCCTTGCAAAGTTGAAGATCTGCAGGATATTGGCGTCGGGGATGTGATC‑TACCTGCCCGGTACGCGGGTG‑CCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTGACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCAGCGTGCGCAAAAACCGCGACAAATGGga                                                                                                                                                                                                                         <  2:330319/245‑1 (MQ=255)                      cACCTTTGAAAATCTGTCTCACACCGGGAGCGCGTTATGAAAAAGATCCTGAAAACCCCGAT‑CAAAGCGGAAGATCTGCAAGATATTCGCGGCGGCGATGTGATC‑TACCTGCCCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGgaga                                                                                                                                                                                                                       <  2:304453/249‑1 (MQ=255)                      cACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTAGGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGgaga                                                                                                                                                                                                                       <  2:299585/249‑1 (MQ=255)                           ttGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtctc                                                                                                                                                                                                                >  2:294615/1‑251 (MQ=255)                               aaaTCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTc                                                                                                                                                                                                            <  1:252730/251‑1 (MQ=255)                                     gTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGCCAAATGGGAGATGGTCTCCGCCGGcccc                                                                                                                                                                                                      >  2:121128/1‑250 (MQ=255)                                          ccccccGGAGCGCGTTATTAAAAAGATCCTTACCACCCCGAT‑CAAAGCTGAAGTGCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCTGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAc                                                                                                                                                                                                 <  1:202989/248‑1 (MQ=255)                                          ccacCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAc                                                                                                                                                                                                 <  1:78445/250‑1 (MQ=255)                                            acCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAcc                                                                                                                                                                                                >  1:184837/1‑250 (MQ=255)                                             cccGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTAGGCGATGTGATC‑TACCTGACAGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCAAGCTG‑CACCATCGTGCGCAAAACCGGCGACAAATGGGAGATGGTCTCCGTCGGCCCgcaaacc                                                                                                                                                                                                >  2:357863/1‑243 (MQ=255)                                                ggacggcgTTATGAAAAAGATCCGGCTACCCCCGAT‑CAAAGCGGAAGTTTTGCAGGTTATCCGCGCCGCCGATGTGATC‑TCCCTGACCGGTACGCTGTGG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAt                                                                                                                                                                                           <  2:206167/246‑1 (MQ=255)                                                   gcgcGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGGGATC‑TACCTGCCCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCg                                                                                                                                                                                        <  1:365368/251‑1 (MQ=255)                                                      cgTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTAt                                                                                                                                                                                     >  1:250869/1‑251 (MQ=255)                                                          aTGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGa                                                                                                                                                                                  <  1:311008/250‑1 (MQ=255)                                                          aTGAAAAAGACCCTGACACCCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCGAACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCTACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGa                                                                                                                                                                                  <  1:367020/250‑1 (MQ=255)                                                                       cgaccacccccgtccaatactacaGATCTGCAAGATATTCGGCTGGGCAAT‑TGGTCCTACCTGACCGGGCCGCTG‑TGCACCTGCCGCGACGTGTGTCACCGCCGTTTGATCGAACTGAAACGTCCGAGCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACGCCCAGGATGCGTATGGAAAGTTTTGAACGt                                                                                                                                                                     <  2:303653/228‑1 (MQ=255)                                                                         aCAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGa                                                                                                                                                                   >  1:216961/1‑250 (MQ=255)                                                                            aCCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAAttt                                                                                                                                                               >  2:78037/1‑251 (MQ=255)                                                                              cccGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTAt                                                                                                                                                             >  2:367919/1‑251 (MQ=255)                                                                              cccGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTAt                                                                                                                                                             <  1:75386/251‑1 (MQ=255)                                                                                        aGCTGAAGATCTGCAAGATATCCGGGCGGGCGATGTGATC‑TACCGGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGAc                                                                                                                                                    <  2:306822/251‑1 (MQ=255)                                                                                            gAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGc                                                                                                                                                >  2:253555/1‑251 (MQ=255)                                                                                                aTCTGCAAGATATTCGCGTCGGCGCTGTGATC‑TACCTGACCGGAACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGCTCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAAAGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTg                                                                                                                                             >  1:205915/1‑250 (MQ=255)                                                                                                      aaGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtgg                                                                                                                                      >  1:368696/1‑251 (MQ=255)                                                                                                                    ggCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggt                                                                                                                        >  1:210301/1‑251 (MQ=255)                                                                                                                    ggCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggg                                                                                                                        >  1:111131/1‑250 (MQ=255)                                                                                                                       gATGTGATC‑TACCTGTCCGGTACGCTGGGG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAGCTGAAACGTCCGATCCCTTACGAGCGCAACGGCAAAGCGATTTTCCACGCTG‑CCCGAGCGGGCTCAAAAACGTCGACAAAGTGGAGATGGTCTCCGTCGGCCCGAGAACCAGGATGCTTATGGAAAGTTTTGAGCTGGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggtggt                                                                                                                     <  1:207625/251‑1 (MQ=255)                                                                                                                                  aCCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGc                                                                                                           >  1:77601/1‑251 (MQ=255)                                                                                                                                   ccTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCt                                                                                                          <  1:362300/251‑1 (MQ=255)                                                                                                                                        ccGGTACCCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCg                                                                                                     <  1:10236/251‑1 (MQ=255)                                                                                                                                                  ggTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGc                                                                                             <  1:141372/249‑1 (MQ=255)                                                                                                                                                  ggTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCt                                                                                            <  1:288128/250‑1 (MQ=255)                                                                                                                                                                      tGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCt                                                                         >  1:321717/1‑250 (MQ=255)                                                                                                                                                                          cccgccgGTTGATCGAACGGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGAGTTTCCACGCTG‑CCCCATCGGGCGCAAAAACGGCGACAAATGTGAGATGGTCTCCGGCGGCCGGACAACCAGTATGCGGATGGAAAGTTTTGATCGGGAATTTAGTGAGCAGTCCGGCGTGAAACTGGTGGTTGGCAGAGGTGGTATGGGGCCGCTCACCTAAGAAGGTTGCCAGAAATTCAAGGTGCTATAtg                                                                    <  2:212243/250‑1 (MQ=255)                                                                                                                                                                           ccgccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCGGCCAGAAATTCAAGGCGCTACAtgt                                                                   >  2:83263/1‑251 (MQ=255)                                                                                                                                                                             gccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGa                                                                                                                                                          <  1:97908/162‑1 (MQ=255)                                                                                                                                                                             gccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGa                                                                                                                                                          >  2:97908/1‑162 (MQ=255)                                                                                                                                                                                  ttGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAtttt                                                             <  2:31717/250‑1 (MQ=255)                                                                                                                                                                                    gATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATTTGATTTTcc                                                           <  1:111570/250‑1 (MQ=255)                                                                                                                                                                                            tGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGAGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCTAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCt                                                  >  1:15203/1‑251 (MQ=255)                                                                                                                                                                                             gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCt                                                  <  2:354982/250‑1 (MQ=255)                                                                                                                                                                                              aaaCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTgc                                                >  1:236102/1‑251 (MQ=255)                                                                                                                                                                                                      gATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCt                                        >  2:101390/1‑251 (MQ=255)                                                                                                                                                                                                         cccTTACGAACTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggc                                     >  2:178852/1‑251 (MQ=255)                                                                                                                                                                                                          ccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggcg                                    <  2:77601/251‑1 (MQ=255)                                                                                                                                                                                                            ttACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTgg                                      <  2:322297/247‑1 (MQ=255)                                                                                                                                                                                                            ttACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTgg                                      >  1:322297/1‑247 (MQ=255)                                                                                                                                                                                                                        cGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTggg                      >  2:95624/1‑250 (MQ=255)                                                                                                                                                                                                                         ggCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATgggg                                                                                                               <  1:141408/161‑1 (MQ=255)                                                                                                                                                                                                                         ggCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATgggg                                                                                                               >  2:141408/1‑161 (MQ=255)                                                                                                                                                                                                                         ggCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGa                      >  1:159164/1‑250 (MQ=255)                                                                                                                                                                                                                           cAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGGGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCACGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGa                      >  1:269034/1‑248 (MQ=255)                                                                                                                                                                                                                               gCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAGACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGAtt               <  2:261025/251‑1 (MQ=255)                                                                                                                                                                                                                               gCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGAtt               <  1:341027/251‑1 (MQ=255)                                                                                                                                                                                                                                        cACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAACTGGGCGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGCAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGAGGTTGGCAAAGGTGGTATAGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGATACATGTGATTTTCCCGGCGGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGCTTGAAGAAGTg      >  2:59202/1‑251 (MQ=255)                                                                                                                                                                                                                                               gCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACt  <  2:173855/249‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                         GCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGAT‑CAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATC‑TACCTGACCGGTACGCTGGTG‑ACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACT  >  NC_000913/3207314‑3207797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑