Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,418,060	C→T	D132N (GAT→AAT)	ulaR ←	DNA‑binding transcriptional repressor UlaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,418,060	0	C	T	100.0%	123.2 / NA	33	D132N (GAT→AAT)	ulaR	DNA‑binding transcriptional repressor UlaR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/13);  total (20/13)

TTACTGCTATCAACCAGTACCACCAGTTTCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCAGCGCGGGCGCTGTTGGGTAATAGCTTCTGCGCCATTGCGCAC  >  NC_000913/4417828‑4418300                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  ttACTGCTATCAACCAGTACCACCAGTTTCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGcc                                                                                                                                                                                                                                   >  2:149347/1‑249 (MQ=255)  tACTGCTATCAACCAGTACCACCAGTTTCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCa                                                                                                                                                                                                                                  <  1:193346/249‑1 (MQ=255)         aTCAACCAGTACCACCAGTTTCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGAt                                                                                                                                                                                                                         <  1:149347/251‑1 (MQ=255)              ccAGTACCACCAGTTTCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTa                                                                                                                                                                                                                    <  1:202522/251‑1 (MQ=255)                 gTACCACCAGTTTCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGtg                                                                                                                                                                                                                 <  2:150402/251‑1 (MQ=255)                     caccaGTTTCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGtgatg                                                                                                                                                                                                              >  2:347958/1‑250 (MQ=255)                            ttCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCa                                                                                                                                                                                                       <  1:37556/250‑1 (MQ=255)                            ttCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCAc                                                                                                                                                                                                      >  2:29308/1‑251 (MQ=255)                               ccTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTgg                                                                                                                                                                                                   >  2:62184/1‑251 (MQ=255)                                                          gcCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAg                                                                                                                                                                        <  1:70707/251‑1 (MQ=255)                                                                      aGCATATCGGTTTTATACAACCCTTCTGCGGTACGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCCTAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCCCGCTGTCATGTTCTTGATTGATCAGGTACTTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTgg                                                                                                                                                            >  2:188989/1‑250 (MQ=255)                                                                      aGCATATCGGTTTTAGACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGa                                                                                                                                                           <  2:104797/251‑1 (MQ=255)                                                                                        aaCCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTAc                                                                                                                                         >  1:507/1‑251 (MQ=255)                                                                                                                ccGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCt                                                                                                                  <  2:7057/250‑1 (MQ=255)                                                                                                                   cTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCg                                                                                                               >  1:204816/1‑250 (MQ=255)                                                                                                                    tGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCAGTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCg                                                                                                               >  1:251359/1‑249 (MQ=255)                                                                                                                          aCATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCt                                                                                                       <  2:24253/251‑1 (MQ=255)                                                                                                                            aTCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCttt                                                                                                     >  1:105008/1‑251 (MQ=255)                                                                                                                                   gCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATa                                                                                              <  2:82060/251‑1 (MQ=255)                                                                                                                                          aTAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGACGCTTTAGCGATACGTACtt                                                                                       >  1:323011/1‑251 (MQ=255)                                                                                                                                             gagaCTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACttttt                                                                                    >  2:206847/1‑251 (MQ=255)                                                                                                                                                   gTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGt                                                                              >  1:12822/1‑251 (MQ=255)                                                                                                                                                    ttttCGCTGCCCTGCGGGCTTAAAGTGCTGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTg                                                                             >  1:323131/1‑251 (MQ=255)                                                                                                                                                                   gtgcttccctttctGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGCCAAC‑AGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCATCTTCTAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATg                                                              >  1:153250/14‑251 (MQ=255)                                                                                                                                                                              gATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCg                                                   >  1:302553/1‑251 (MQ=255)                                                                                                                                                                              gATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCg                                                   >  1:302532/1‑251 (MQ=255)                                                                                                                                                                                         ttttGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCAgcgc                                        >  1:156454/1‑251 (MQ=255)                                                                                                                                                                                                     gTCCGCCCATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCATCGCGGGCGCTGtt                              <  2:160728/249‑1 (MQ=255)                                                                                                                                                                                                          cccATAATGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCAGCGCGGGCGCTGTTGGGTAAt                       >  1:125433/1‑251 (MQ=255)                                                                                                                                                                                                               aaTGATCACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCAGCGCGGGCGCTGTTGTGTAATAGCt                   <  2:113419/250‑1 (MQ=255)                                                                                                                                                                                                                     cACGCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCAGCGCGGGCGCTGTTGGGTAATAGCTTCTgcg             <  1:185314/250‑1 (MQ=255)                                                                                                                                                                                                                        gCTGTCATGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCAGCGCGGGCGCTGTTGGGTAATAGCTTCTGCGCCAt         >  1:63307/1‑251 (MQ=255)                                                                                                                                                                                                                               tGTTCTTGATTGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCAGCGCGGGCGCTGTTGGGTAATAGCTTCTGCGCCATTGCGCAc  >  2:22584/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  TTACTGCTATCAACCAGTACCACCAGTTTCCCTACCACGCTCAGCATCTTCTGCTCTGCCATTGCTGTCAGCATATCGGTTTTATACAACCCTTCTGCGGTCAGCCCTTTTCCGCTGGTAAACATCCAGTGCCCGGCATAGAGACTGTTTTCGCTGCCCTGCGGGCTTAAAGTGATGGACTGACTTTTGTTGTACTGTCCGCCCATAATGATCACGCTGTCATGTTCTTGATCGATCAGGTAATTTGCCAGCGGTAGATAATTAGTGATGATTTGCACTGGCTTGCCACACATTTCCCGCCCAAGCAGA‑AACGCGGTGGAGCCGCAGTTGATGACTACGCTTTCGCCCGGATTAACCAGCTGCGAGGCCGCTTTAGCGATACGTACTTTTTCATCGTGATTCTGCGCCTGATGCAGATTCATCGGCGTCCAGCGCGGGCGCTGTTGGGTAATAGCTTCTGCGCCATTGCGCAC  >  NC_000913/4417828‑4418300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑