Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,124,375	G→A	R28* (CGA→TGA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,124,375	0	G	A	100.0%	81.0 / NA	24	R28* (CGA→TGA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/12);  total (12/12)

GATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGG‑ACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAATTTTTCGATCCGCCTCGCATCGTGAGCGGTTT  >  NC_000913/4124129‑4124613                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               gATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGG‑ACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGga                                                                                                                                                                                                                                             <  2:49049/250‑1 (MQ=255)           ggcACAGTAGCCAATCAGCCACAGATTTACGTGAGTTGCCGCCGTAAACTCGATACCGGGAATAATTTCGCTAAAGAAGGGATTGACGATATACGGGCACATTC‑CCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACGTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTg                                                                                                                                                                                                                                  <  1:140087/250‑1 (MQ=255)                                                                               cGCTAAAGAAGGGATCGCAGATATCCGGG‑ACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTcaca                                                                                                                                                              <  2:80221/251‑1 (MQ=255)                                                                                      gaagGGATCGCAGATATCCGGG‑ACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACactcactc                                                                                                                                                       >  2:56652/1‑251 (MQ=255)                                                                                       acgggATCGCAGATATCCGGG‑ACAATCACCAGAATGGTGCGGGATTCCTTACGCTTGACGTTGCGCCCCACACCCCGCGCTAAATAACCCACTTCCCGCGCCGCTTTTTCCACCCGATTCCGGGCGGCCTGCGCGCCTTTCTCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGCCACTCTTGCCTTCAGGCCCACGCCTTTCATGCTCGCCGCAGCTTCCTCCTCCTTCGCTTTCACCctccctcc                                                                                                                                                      >  2:278538/3‑251 (MQ=255)                                                                                            aTCGCAGATATCCGGG‑ACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCTCTCCTCGcc                                                                                                                                                 <  2:295023/251‑1 (MQ=255)                                                                                                         ggg‑ACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGc                                                                                                                                    <  1:40114/251‑1 (MQ=255)                                                                                                         ggg‑ACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGc                                                                                                                                    >  1:124733/1‑251 (MQ=255)                                                                                                                  cACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGtactac                                                                                                                             <  1:129013/250‑1 (MQ=255)                                                                                                                             tGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTAc                                                                                                                 >  1:199481/1‑251 (MQ=255)                                                                                                                                gggATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTc                                                                                                              >  2:102887/1‑251 (MQ=255)                                                                                                                                      aaTTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCg                                                                                                        <  2:191771/250‑1 (MQ=255)                                                                                                                                                               aGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGt                                                                               >  2:65737/1‑251 (MQ=255)                                                                                                                                                                  cTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGa                                                                             >  2:261822/1‑250 (MQ=255)                                                                                                                                                                     cGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAAc                                                                         >  2:324556/1‑251 (MQ=255)                                                                                                                                                                         aaaTAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGtt                                                                     <  2:154242/251‑1 (MQ=255)                                                                                                                                                                                      cccGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGATTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCAt                                                        <  2:258559/251‑1 (MQ=255)                                                                                                                                                                                                               aCGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATAGTGAAtt                                <  2:54011/250‑1 (MQ=255)                                                                                                                                                                                                                           ggAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGa                                                                             <  2:304476/193‑1 (MQ=255)                                                                                                                                                                                                                           ggAGACTTTATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAATTTTTCGATCCGCCt                   >  2:337059/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttATCGGGATTCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAATTTTTCGATCCGCCTCGCATc             <  1:236204/250‑1 (MQ=255)                                                                                                                                                                                                                                         gATTCATTAATGCTCAGGCGCCGATCGCTGTAGAGACTTTTGCCTTGCGGGCAACGTCTTTCATGGTAGCGGCAGTTTCCTGCTTCTTCGCTTTCACAATCACTCCTCGCCTGGCACGTCAGGCGTAATACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAATTTTTCGATCCGACTCGCATCGTGATCgg     >  2:359766/1‑251 (MQ=255)                                                                                                                                                                                                                                         gATTCATTAATGCTCAGGAGACGGACGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCCGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGCATATTGAATTTTTCGATCCGCCTCGCATCGTGAGCgg     >  2:359752/1‑251 (MQ=255)                                                                                                                                                                                                                                            tCATTAATGCTCAGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAATTTTTCGATCCGCCTCGCATCGTGAGCGGttt  >  1:366685/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGG‑ACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTTCACATCGGCAACATTTTTAGCAGATAGCGCGTGAAAACGGTTACAGAATTTTCATGAAAAGTGTGATGAATATTGAATTTTTCGATCCGCCTCGCATCGTGAGCGGTTT  >  NC_000913/4124129‑4124613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑