Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,093,346	(T)6→5	coding (471/1359 nt)	dgcT →	putative diguanylate cyclase DgcT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,093,346	0	T	.	100.0%	90.8 / NA	20	coding (471/1359 nt)	dgcT	putative diguanylate cyclase DgcT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/12);  total (8/12)

TAGTTTGGCTTTTCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTG  >  NC_000913/1093106‑1093589                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    tAGTTTGGCTTTTCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTgggg                                                                                                                                                                                                                                           <  2:143380/250‑1 (MQ=255)                                                          gCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGc                                                                                                                                                                                 >  2:309600/1‑251 (MQ=255)                                                              attattGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCCATCTATTATTTGTTTCGTCAGTTGAGTTTGTGCCTATTCATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCg                                                                                                                                                                               >  2:330643/1‑249 (MQ=255)                                                                 attGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGtt                                                                                                                                                                           <  1:85243/250‑1 (MQ=255)                                                                 attGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGtt                                                                                                                                                                           <  2:15106/250‑1 (MQ=255)                                                                    gaggagCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCAATCTATTATTTGGTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCGCACATACTTTCTAGTCATTATGAGAGCTACAATTTACCCATTGCCGAGTTAAc                                                                                                                                                                        <  2:305680/250‑1 (MQ=255)                                                                     aggagCGTTTGACAAGCAGTGTTGTCCAAACTGACA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAAcc                                                                                                                                                                       >  1:338645/1‑250 (MQ=255)                                                                       gagCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAAt                                                                                                                                                                    <  1:7792/251‑1 (MQ=255)                                                                                               cAAAATGACAATTG‑AATTTAGTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAATACACAAAACAGCGGCAGTTATTTTCCAAAAAAATGACTTTGTGGAGAAGTTTGTTTTT‑GTTTTTGGGGAGCCGATATTTGCTCACATGCTTTCCAGTCATTATGAGAGCTCGAATTTACACATTGCCGAGTTAACAAATGTAAAGGGTGAGGTGGGTTGGaaa                                                                                                                                            <  2:47840/251‑1 (MQ=255)                                                                                                      aCA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCat                                                                                                                                     >  2:75858/1‑251 (MQ=255)                                                                                                                                 cAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAATTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCAtgtg                                                                                                            <  1:317618/250‑1 (MQ=255)                                                                                                                                 cAGATGAGATTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTgg                                                                                                           >  1:298011/1‑251 (MQ=255)                                                                                                                                               cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCTCATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTc                                                                                             >  2:251928/1‑251 (MQ=255)                                                                                                                                               cATATTAAGACTTCTGGAATTGGTGCATACCGTTCGTGAAACCATAAAACAGCACACTTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGCAAGTGGTCAGGTGGTTTAGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTc                                                                                             >  2:148504/1‑251 (MQ=255)                                                                                                                                                       tatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATc                                                                                     >  2:300541/1‑251 (MQ=255)                                                                                                                                                                     gTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGt                                                                       <  2:14643/251‑1 (MQ=255)                                                                                                                                                                                               aCAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTGCCATAATGATTTTCATGTGGTTAACCGTGCTTTCGGTTAATCGATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGt                                             <  1:38919/251‑1 (MQ=255)                                                                                                                                                                                                                    aaaaaTGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGc                        <  1:153291/251‑1 (MQ=255)                                                                                                                                                                                                                              ttGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTa               <  1:284952/250‑1 (MQ=255)                                                                                                                                                                                                                                gtgCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACaa            <  2:338645/251‑1 (MQ=255)                                                                                                                                                                                                                                           ttgtttttgtttttgGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATctctct   <  1:67307/247‑1 (MQ=255)                                                                                                                                                                                                                                            tgtttttgtttttgGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTg  <  1:124165/248‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    TAGTTTGGCTTTTCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACA‑TTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTG  >  NC_000913/1093106‑1093589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑