Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,189,636 G→A intergenic (+63/‑150) rpoC → / → yjaZ RNA polymerase subunit beta'/protein YjaZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,189,6360GA96.9% 95.5 / NA 32intergenic (+63/‑150)rpoC/yjaZRNA polymerase subunit beta'/protein YjaZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/17);  minor base C (1/0);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGT  >  NC_000913/4189393‑4189874
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
agagAACGTTATCGTGGGTCGTCTGAGCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAagag                                                                                                                                                                                                                                          <  1:273863/251‑1 (MQ=255)
   gaACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCAt                                                                                                                                                                                                                                       <  1:285552/251‑1 (MQ=255)
         tATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGAGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTc                                                                                                                                                                                                                                  <  2:25506/250‑1 (MQ=255)
                                         gtgaCGCGTTCCCCCATGATCGTATGCGTCGCCGTGTTTCAGGTGAAGCGCCTGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCATCCTGGCAGAACTGCTGAACGCATGGCTGGGTGGGTCTGATAACGAGTAATCGGGAATCCGCAAAGAACGTAAAAACCCGCTTCGGCTGG‑TTTTTTTATGGGGTGAGTTTAGAGAAAGAGCATTTGTGAGAATATTTAAAGAATTTGTGAATACGCATaag                                                                                                                                                                                                 <  2:333721/248‑2 (MQ=255)
                                         gTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAt                                                                                                                                                                                                 <  1:36312/251‑1 (MQ=255)
                                          ttACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGATCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTATGAATACTCATAATc                                                                                                                                                                                                >  1:226136/1‑251 (MQ=255)
                                            aCGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCa                                                                                                                                                                                               <  2:314537/250‑1 (MQ=255)
                                               cgTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGt                                                                                                                                                                                           <  2:129628/251‑1 (MQ=255)
                                                      ccaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATg                                                                                                                                                                                    >  2:45795/1‑251 (MQ=255)
                                                                  gCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCCTAATCAATGTAGAGATGACTAATATCCT‑g                                                                                                                                                                       >  1:98263/1‑251 (MQ=255)
                                                                                   gTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAAt                                                                                                                                                      >  1:356139/1‑251 (MQ=255)
                                                                                                 gCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTATGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGTGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTTAATACTCATAATCAATGTAGAGATGTCTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCg                                                                                                                                         <  1:310833/250‑1 (MQ=255)
                                                                                                                 cAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTa                                                                                                                        <  1:372047/251‑1 (MQ=255)
                                                                                                                     agaCGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGGTTTTTTTATTGGGGGAGTTTAAAGAAAGAGCATTTGTCAGAAAATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑TAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTc                                                                                                                     >  2:26195/1‑251 (MQ=255)
                                                                                                                        cGCATCTGGCAGCATGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGGTTAGAGAAAAAGAATTTGTCAGAATTTTTTAGGAATTTTTTGATACTGATAATCTATGTAGAGAAGGCTAATATCCTTGAACATAAGT‑AACTAATTTAGTCAAACTCGGGAAGGATTCGATCCTCTTCCtgt                                                                                                                 >  2:344701/1‑251 (MQ=255)
                                                                                                                                      tGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCttt                                                                                                     >  1:181691/1‑248 (MQ=255)
                                                                                                                                       ggCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAg                                                                                                   <  2:291293/250‑1 (MQ=255)
                                                                                                                                                   gAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGa                                                                                       <  2:1973/250‑1 (MQ=255)
                                                                                                                                                               gggCGGTTCTGATAACGAGTAATCGTTAATCCGGAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAAGCAATGTAGAGATGACTAATAGCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTAGTCCTGTGTAACTTGCTTAAGGAACGAGAATGAAACAGGAAGt                                                                             <  2:211736/248‑1 (MQ=255)
                                                                                                                                                               gggCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTgg                                                                           >  1:370862/1‑250 (MQ=255)
                                                                                                                                                                               gAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCtttt                                                          <  1:45795/251‑1 (MQ=255)
                                                                                                                                                                                          aaTCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑GTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAAAAGGAAGTGGAAAAGAGGCGACCTTTTGGACATCCgg                                                >  1:191104/1‑250 (MQ=255)
                                                                                                                                                                                             ccGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATgg                                            <  2:356139/251‑1 (MQ=255)
                                                                                                                                                                                              cGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGt                                           <  2:29798/251‑1 (MQ=255)
                                                                                                                                                                                                      aCGTAAAAACCCGCTTCGGCGGG‑TTTTTTTTTGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCg                                   >  1:65440/1‑251 (MQ=255)
                                                                                                                                                                                                        gTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTg                                 <  1:36642/251‑1 (MQ=255)
                                                                                                                                                                                                            aaaCCCGCTTCGGCGGG‑ATTTTTTATGGGGGGAGATTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATAATCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCAGGATGGTGATATTCGTGAttt                             >  1:307872/1‑251 (MQ=255)
                                                                                                                                                                                                                       ggcggG‑TTTTTTTATGGGGGGGGTTTAGCGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCACTGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACCGGAAGTGGAAAAGGGGCGACCTTTTGGACATCTGGATGGGGATATTCGTGATTTATCATTTCttt                  >  2:18228/1‑250 (MQ=255)
                                                                                                                                                                                                                        gcggG‑TTTTTTTATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGa                 <  2:98263/251‑1 (MQ=255)
                                                                                                                                                                                                                                    tATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAgg      >  2:304048/1‑251 (MQ=255)
                                                                                                                                                                                                                                    tATGGGGGGAGTTTAGAGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAgg      >  1:352695/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gggggAGTTTAGAGAAAGAGCATTTGTCAGCATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGt  >  2:154655/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
AGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGG‑TTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCT‑GAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGT  >  NC_000913/4189393‑4189874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑