Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,054,219	G→A	P354S (CCA→TCA)	glnG ←	DNA‑binding transcriptional dual regulator NtrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,054,219	0	G	A	100.0%	153.9 / NA	45	P354S (CCA→TCA)	glnG	DNA‑binding transcriptional dual regulator NtrC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/19);  total (26/19)

CCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCAC  >  NC_000913/4053986‑4054459                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 ccGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTc                                                                                                                                                                                                                                  >  1:207986/1‑250 (MQ=255)           tctGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTc                                                                                                                                                                                                                       >  1:349031/1‑251 (MQ=255)                    gcTTCGGAACGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCCGAGCAGCTTCGGTTTCCgg                                                                                                                                                                                                              >  2:204458/1‑251 (MQ=255)                        cGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGc                                                                                                                                                                                                          >  1:170453/1‑251 (MQ=255)                            aaGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTGCCGGATGCAGTa                                                                                                                                                                                                      <  2:30763/251‑1 (MQ=255)                                          gaCCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTAc                                                                                                                                                                                         <  1:98242/250‑1 (MQ=255)                                              ggAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGcccc                                                                                                                                                                                    >  2:77105/1‑250 (MQ=255)                                               gAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTTCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGGGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGCGCAGCTTCGGTTTCCGGATTCAGTAACTTCGCTTCTACGccc                                                                                                                                                                                     >  2:257368/1‑249 (MQ=255)                                                  cGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACAGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTc                                                                                                                                                                                >  2:344160/1‑251 (MQ=255)                                                     aGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTcgcg                                                                                                                                                                             >  1:256725/1‑251 (MQ=255)                                                           ctgtctgCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTcgcgcgcg                                                                                                                                                                         <  1:66238/249‑1 (MQ=255)                                                                  cccACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGt                                                                                                                                                                >  1:337446/1‑251 (MQ=255)                                                                   ccACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGt                                                                                                                                                                >  1:143713/1‑250 (MQ=255)                                                                         cgcAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAAt                                                                                                                                                         >  2:49220/1‑251 (MQ=255)                                                                            aagaagCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGgc                                                                                                                                                      >  1:279253/1‑251 (MQ=255)                                                                                  cGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGccc                                                                                                                                                >  2:117698/1‑250 (MQ=255)                                                                                         cAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCACCACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACgggg                                                                                                                                         >  2:176383/1‑251 (MQ=255)                                                                                         cAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGAGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACgggg                                                                                                                                         >  1:291549/1‑251 (MQ=255)                                                                                                             cGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTcgc                                                                                                                     >  1:29120/1‑251 (MQ=255)                                                                                                                aGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCa                                                                                                                  <  1:346884/251‑1 (MQ=255)                                                                                                                       tcCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCCTCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCCCGTTGACTGACCCCGCCAGACGCGTAAGAGCCGCTTCGGTTTCCGGATGCAGTCACTTCGCTTCCACGCCCCGTTCGCGCGCGGCAACCTGTACAAAATGGCGCGCCAGACGTGGAATATCTTCCCGACGTTCGCGCAgcgtccg                                                                                                           >  2:358404/1‑247 (MQ=255)                                                                                                                        ccGCACCCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCAAGTTGCCTTACCACGCCAGACGCGCCAGAGCAGCTTCGGTTTTCGGATGCCGTAAATTAGCTTCTACGACCAGTTCCCGATCGGAAACCTGTAAAAAATGGCGCGCCAGGCGGGGAATATCTTCCCGACGTTCGCGCAGCggcggc                                                                                                          >  2:217120/1‑251 (MQ=255)                                                                                                                                gTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCCTCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGCGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAAc                                                                                                  >  1:47940/1‑251 (MQ=255)                                                                                                                                       cAAACATTTCGCCGGTCAAATTCTGATTCATCACTTCCCGCCCGGAGGCAATCCCCGTTAGACAGCTGCAGGTGTTTGCCAGCTGTCGCACGTTGCCTGACAACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGTCGGGTAATATCTTCCCTACGTTCGCGCAGCGGCGGCAGATGACCGCGGAt                                                                                            <  2:204086/250‑1 (MQ=255)                                                                                                                                       cAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATa                                                                                           <  1:352816/251‑1 (MQ=255)                                                                                                                                                    gggCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGt                                                                              <  2:195564/251‑1 (MQ=255)                                                                                                                                                     ggCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTg                                                                             <  1:33245/251‑1 (MQ=255)                                                                                                                                                            ccTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGa                                                                      <  1:77105/251‑1 (MQ=255)                                                                                                                                                             cTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTT‑CCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAAc                                                                         >  1:32097/1‑246 (MQ=255)                                                                                                                                                                   cAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGc                                                                                 >  2:79201/1‑233 (MQ=255)                                                                                                                                                                   cAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGc                                                                                 <  1:79201/233‑1 (MQ=255)                                                                                                                                                                        cTTCCTGCCCGGCGGCCATCACCGTTAGCCCGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTTACCACCCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGCTGCAGTAACTTCGCTTCTACGCCCCGTTCGCGCGCGGCAAACTGTAAAAAATGGCGCGCCAGACGGGGAATCTCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCTGATAACGTTCAGGCCGTGGAACCGATCCTCACggccc                                                          >  2:132550/1‑248 (MQ=255)                                                                                                                                                                           ccTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACttt                                                       >  2:40371/1‑250 (MQ=255)                                                                                                                                                                                   gcggcCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTg                                               <  1:363268/251‑1 (MQ=255)                                                                                                                                                                                              cGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAgg                                                                                  <  2:270393/205‑1 (MQ=255)                                                                                                                                                                                              cGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAgg                                                                                  >  1:270393/1‑205 (MQ=255)                                                                                                                                                                                               gTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTGCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTc                                   <  2:337446/251‑1 (MQ=255)                                                                                                                                                                                                  aGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCgag                                <  1:309129/251‑1 (MQ=255)                                                                                                                                                                                                     cAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGAtt                             <  1:358803/251‑1 (MQ=255)                                                                                                                                                                                                      aGCGGCATGTGTTTTCCAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCTCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTc                            <  1:97877/251‑1 (MQ=255)                                                                                                                                                                                                        cGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCGGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCTCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGGTCGAGATTCt                           <  2:295489/250‑1 (MQ=255)                                                                                                                                                                                                                      cAGCTGGCGCACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCg             >  1:27678/1‑250 (MQ=255)                                                                                                                                                                                                                            gcgcACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATcc      <  2:167399/251‑1 (MQ=255)                                                                                                                                                                                                                               cACGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCACCCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGc    >  1:364109/1‑250 (MQ=255)                                                                                                                                                                                                                                aCGTTGCCTGACCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGcac  <  1:49220/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 CCGCTCCAGCTCTGGCTGCGCTTCGGAAAGCAGATTTTGATGACCGGAACGCAGCGCTCTGTCTGCCCACTGCGCAAGAAGCGTCGCCCAGCTGTCCGGTTGCATTTGCGAAGTACTCTCCGCAACCGTTGATTCAAACAGTTCGCCGGGCAAATCCTGAATCAACACTTCCTGCCCGGCGGCCATCACCGTTAGCCAGCGGCAGGTGTTTTCCAGCTGGCGCACGTTGCCTGGCCACGCCAGACGCGTCAGAGCAGCTTCGGTTTCCGGATGCAGTAACTTCGCTTCTACGCCCAGTTCGCGCGCGGCAACCTGTAAAAAATGGCGCGCCAGACGGGGAATATCTTCCCGACGTTCGCGCAGCGGCGGCAGATGAACGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCAC  >  NC_000913/4053986‑4054459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑