Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,548	C→T	S331F (TCC→TTC)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,548	0	C	T	100.0%	168.0 / NA	49	S331F (TCC→TTC)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/27);  total (22/27)

TCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGT  >  NC_000913/1680310‑1680787                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                tCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCGGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACtgt                                                                                                                                                                                                                                      <  1:58436/250‑1 (MQ=255)      gggTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTg                                                                                                                                                                                                                                 >  2:314108/1‑250 (MQ=255)      gggTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTg                                                                                                                                                                                                                                 <  1:74970/250‑1 (MQ=255)       ggTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGAc                                                                                                                                                                                                                               >  1:122322/1‑251 (MQ=255)        gtgtGGTGTCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATCAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACATGCTTCGCCAGTCGCCTCACTGTGGTTGAcc                                                                                                                                                                                                                              <  2:89659/251‑1 (MQ=255)        gtgtGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGAcc                                                                                                                                                                                                                              <  2:364267/251‑1 (MQ=255)            ggtggCACGTCCTGAACTGGCTGAAATTAGTAACACGACAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACAAAc                                                                                                                                                                                                                           >  1:15075/1‑250 (MQ=255)                gCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTg                                                                                                                                                                                                                      <  2:59398/251‑1 (MQ=255)                   cGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGt                                                                                                                                                                                                                   <  1:354005/251‑1 (MQ=255)                      ccTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCCGCGTGc                                                                                                                                                                                                                 >  2:220422/1‑250 (MQ=255)                                  gAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGt                                                                                                                                                                                                    >  1:186493/1‑251 (MQ=255)                                       tCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTc                                                                                                                                                                                                <  2:268087/250‑1 (MQ=255)                                              ccGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCt                                                                                                                                                                                        <  1:127742/251‑1 (MQ=255)                                               cGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACt                                                                                                                                                                                                                                        >  1:153164/1‑202 (MQ=255)                                               cGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACt                                                                                                                                                                                                                                        <  2:153164/202‑1 (MQ=255)                                               cGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTg                                                                                                                                                                                       >  2:258437/1‑251 (MQ=255)                                                 gcAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGTCTGAc                                                                                                                                                                                     <  2:311473/250‑1 (MQ=255)                                                    aTGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTTCGCCATTCGCCTCGCTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCgg                                                                                                                                                                                  <  1:224793/251‑1 (MQ=255)                                                      ggCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGtt                                                                                                                                                                                >  1:108488/1‑251 (MQ=255)                                                          ggtatGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGAAGAAGTACCGTTCCTGGCCACCACTCATAAAGCATTACCGCGCATTTTTGCGCGCCAGAACGCACAATCTGCGCCATTCCCCTCACTGGGGTTGACCAACATCTGCGTGCCAATTTGTCTGGGCCTCATCTGGCTGACCGGTTCCg                                                                                                                                                                             >  1:244533/5‑250 (MQ=255)                                                          ggTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCg                                                                                                                                                                             >  1:244522/1‑250 (MQ=255)                                                               gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTac                                                                                                                                                                        >  2:195003/1‑250 (MQ=255)                                                                   gtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAacac                                                                                                                                                                   >  1:258147/1‑251 (MQ=255)                                                                       aaaTGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTg                                                                                                                                                               >  2:290932/1‑251 (MQ=255)                                                                              gggACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGAcc                                                                                                                                                         <  1:75455/250‑1 (MQ=255)                                                                                    gtGGGGCGAAATCATCATCGCTGCCGGTTGGATCGTTTCCGTGTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTTCAAATTTGTCTGTTTCTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCt                                                                                                                                                  <  2:216498/250‑1 (MQ=255)                                                                                            aaaTCATCATCGCTGCCTGTTTGATCGTTTCCGTTTGCGGCTCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCAGAAAGCATTCCCTCGCATTTTTGCGCGCCATAACGCACAAGCTGCGCCATTCGCCTCGCTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCTATTACAACACGTTGCTGACCATCGCCTCAGAAATg                                                                                                                                          <  1:254548/251‑1 (MQ=255)                                                                                                catcatcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTc                                                                                                                                      >  1:124429/1‑251 (MQ=255)                                                                                                                   aTCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGt                                                                                                                   <  2:280931/251‑1 (MQ=255)                                                                                                                     cGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTc                                                                                                                  <  2:364991/250‑1 (MQ=255)                                                                                                                            gTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGtt                                                                                                          <  2:146723/251‑1 (MQ=255)                                                                                                                                   gcgcGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTg                                                                                                   <  2:18440/251‑1 (MQ=255)                                                                                                                                   gcgcGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTg                                                                                                   <  1:203071/251‑1 (MQ=255)                                                                                                                                            tAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCg                                                                                            <  2:169870/249‑1 (MQ=255)                                                                                                                                              agttGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACTCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCTGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAc                                                                                        <  1:235043/248‑1 (MQ=255)                                                                                                                                                    aCCATCATGGCATCCGAAGTACCTTTCCTGGTCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCCTTCTTGCTGAAACTCGCAACCCGTca                                                                                  <  2:83633/251‑2 (MQ=255)                                                                                                                                                          atGGCAGCAGAAGTACCGTTCGTGGCCGCCACTAATAACGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCAGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTAATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGGGCCCTATTTCCTGGTCTGCGCGTTCTGGCTGAAAATCGCAACCCGTCCACTGc                                                                             >  2:333466/1‑250 (MQ=255)                                                                                                                                                            ggCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCAt                                                                           <  2:124429/250‑1 (MQ=255)                                                                                                                                                                                tGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGcc                                                      >  1:134075/1‑251 (MQ=255)                                                                                                                                                                                     gccACTAATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACACGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGACTGCAt                                                 >  2:322758/1‑251 (MQ=255)                                                                                                                                                                                              caaGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATATGCGTGCAAATGTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAACCGTCTACTGCATAAAGCGGTAGTTGTCGGTCCCTGCATTTATGCCtt                                        >  2:22711/2‑251 (MQ=255)                                                                                                                                                                                                       ccGCGCATTTTTGCGCGCCCGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATgg                                    >  2:56788/1‑246 (MQ=255)                                                                                                                                                                                                       ccGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATgg                                    <  1:56788/246‑1 (MQ=255)                                                                                                                                                                                                                  tGCGCGCCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCctct                     <  2:108488/250‑1 (MQ=255)                                                                                                                                                                                                                   gcgcgcCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTTCGCCTCTgg                   <  1:314108/251‑1 (MQ=255)                                                                                                                                                                                                                      cgcCAGAACGCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTcc                >  1:262707/1‑251 (MQ=255)                                                                                                                                                                                                                               gCACAAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACtt       >  1:46336/1‑251 (MQ=255)                                                                                                                                                                                                                                 acaAGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTTCTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGc     <  1:372194/251‑1 (MQ=255)                                                                                                                                                                                                                                    aGCTGCGCCATTCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGt  >  1:229676/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                TCGCTGGGTGTGGTGGCACGTCCTGAACTGGCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGGTCCAATGCACTTGCTGT  >  NC_000913/1680310‑1680787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑