Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,457,427	G→A	Q147* (CAA→TAA)	yjgA ←	putative ribosome biogenesis factor YjgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,457,427	0	G	A	100.0%	186.8 / NA	51	Q147* (CAA→TAA)	yjgA	putative ribosome biogenesis factor YjgA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/21);  total (30/21)

ATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTC  >  NC_000913/4457181‑4457664                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              aTGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCATGGTGCGCAGTTACTGa                                                                                                                                                                                                                                           <  1:274207/251‑1 (MQ=255) aTGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGa                                                                                                                                                                                                                                           <  1:146772/251‑1 (MQ=255)   gCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGAc                                                                                                                                                                                                                                          <  1:259372/250‑1 (MQ=255)           cGGATAACGCGTTCACGCCGCATCCGGCATTTGAGCCTACTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCAt                                                                                                                                                                                                                                 >  2:224500/1‑251 (MQ=255)            ggATAATGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATc                                                                                                                                                                                                                                <  1:96334/251‑1 (MQ=255)                 aGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGcc                                                                                                                                                                                                                           <  2:3892/251‑1 (MQ=255)                   gCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCcac                                                                                                                                                                                                                         >  1:123683/1‑251 (MQ=255)                                  ccGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTc                                                                                                                                                                                                           <  1:129911/250‑1 (MQ=255)                                       aTTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCTCAGATTTAATACCTCAGCGAt                                                                                                                                                                                                     >  2:96892/1‑251 (MQ=255)                                                  gTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCAc                                                                                                                                                                                          <  2:35324/251‑1 (MQ=255)                                                      cTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCCTCACCTTg                                                                                                                                                                                      >  2:201379/1‑251 (MQ=255)                                                           gCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTgatcg                                                                                                                                                                                  <  2:224487/250‑1 (MQ=255)                                                                        cGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGAGTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATcgcg                                                                                                                                                                    <  2:371907/251‑1 (MQ=255)                                                                        cGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCCCTAACTCGCCCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTAATTCCCTTCTTTCTTTTTCTTGGCGTTGGGGATCAGAGTACGCAGTTACTGAGGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTATTCGATCAGACGATcgcg                                                                                                                                                                    >  1:59233/1‑251 (MQ=255)                                                                             tATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGt                                                                                                                                                               >  2:63918/1‑251 (MQ=255)                                                                                     cTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGt                                                                                                                                                       >  2:145254/1‑251 (MQ=255)                                                                                        cAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTa                                                                                                                                                    <  2:125320/251‑1 (MQ=255)                                                                                                         gCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACctg                                                                                                                                   <  2:325875/251‑1 (MQ=255)                                                                                                         gCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACctg                                                                                                                                   <  1:77201/251‑1 (MQ=255)                                                                                                          cAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACctgc                                                                                                                                  >  1:189164/1‑251 (MQ=255)                                                                                                            aaCCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTCACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGTGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGTAACAGCACCACctgctg                                                                                                                                >  1:331679/1‑251 (MQ=255)                                                                                                               cATACTCACATCCACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGtt                                                                                                                             >  1:278263/1‑251 (MQ=255)                                                                                                                aTACTCACATCAACAACGACAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGCTTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCTGATCAGAGTACGCAGTTACTGACGATCGGCATCGTGCCACAGATTTAATACCTCAGCGATGGCGTCCTCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGttt                                                                                                                            >  2:158871/1‑250 (MQ=255)                                                                                                                             caacGAACATTACCCTTCGTTCTCCGCTAACTCCCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTc                                                                                                                >  2:47620/1‑250 (MQ=255)                                                                                                                                      ttACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAAAAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCCTATcc                                                                                                       >  1:181044/1‑250 (MQ=255)                                                                                                                                         cccTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAgcg                                                                                                   >  1:9082/1‑251 (MQ=255)                                                                                                                                               gTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGAc                                                                                             >  1:20461/1‑251 (MQ=255)                                                                                                                                                    ccGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAAt                                                                                         >  1:189485/1‑250 (MQ=255)                                                                                                                                                    ccGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAAt                                                                                         <  2:228798/250‑1 (MQ=255)                                                                                                                                                     cGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAg                                                                                       <  2:34994/251‑1 (MQ=255)                                                                                                                                                     cGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAg                                                                                       <  1:65894/251‑1 (MQ=255)                                                                                                                                                         aaCTCGAGCAGATACTGGAAAATCTGGCGTGAGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCTAGTTTATGGAACAGCACCACCTGCTGGATGCGACGGTTTTTCAGATTATCCAGCGCCTGACGAATAGGctc                                                                                   >  2:229028/1‑251 (MQ=255)                                                                                                                                                          aCTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGctct                                                                                  >  2:294217/1‑251 (MQ=255)                                                                                                                                                                    aTACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGTCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCt                                                                        >  1:243034/1‑251 (MQ=255)                                                                                                                                                                           aaaaTCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCgcag                                                                 <  1:217194/251‑1 (MQ=255)                                                                                                                                                                             aaTCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCgcagc                                                                >  2:76469/1‑250 (MQ=255)                                                                                                                                                                             aaTCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCgcagc                                                                >  2:76475/1‑250 (MQ=255)                                                                                                                                                                                        cGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATg                                                    >  1:83777/1‑251 (MQ=255)                                                                                                                                                                                          gATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAg                                                  <  1:124568/251‑1 (MQ=255)                                                                                                                                                                                                ggcggcTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCa                                             >  2:47844/1‑250 (MQ=255)                                                                                                                                                                                                       tATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGTGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggcg                                      <  2:127101/250‑1 (MQ=255)                                                                                                                                                                                                       tATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggcg                                      <  1:286644/250‑1 (MQ=255)                                                                                                                                                                                                        aTTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggcgg                                     <  1:323291/250‑1 (MQ=255)                                                                                                                                                                                                             cttcttTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGgcggcggcg                                >  1:204402/1‑250 (MQ=255)                                                                                                                                                                                                                    ctctttctTCGCGTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTcc                         >  2:133307/1‑250 (MQ=255)                                                                                                                                                                                                                              gcgTTGCGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGTCGGCGGCGACCTTCCATCTTAATACg              >  2:80708/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGGATCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGc        >  2:118429/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTCAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGCTGTGACGGCTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCg       >  2:299375/1‑249 (MQ=255)                                                                                                                                                                                                                                         cAGAGTACGCAGTTACTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCt   <  1:267203/251‑1 (MQ=255)                                                                                                                                                                                                                                          agagTACGCAGTTACTGCCGATCGGCATCGGGCCACAGCTTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCCGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTCCCGATGAGCTGCAACTGGCAGCGGCGCCCTTCCATCTTACTCCGCTGGGagagacc  >  2:347716/1‑248 (MQ=255)                                                                                                                                                                                                                                          agagGACGCAGTTACTCAAGATCGGCATAGGGCAACAGATTTAATACCTCAGCGCTGGCGTCATCACCTAGATAGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGCACAGCACCACCTGCTGGTTGTGCCGGTTTTTCAGCTTATCCAGCGCCTGACGACTAGGCTCTACGTCGCGCTGGCGCAGCATCTTACTGATGAGCTGCAACTGGCGGCGGCGACTTTCCATATTCATACACGGAGCGAGCTc  >  2:347701/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              ATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAATCTGGCGTGCGGATTTCGGCGGCTTATTCCCTTCTTTCTCTTTCTTCGCGTTGCGGATCAGAGTACGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTC  >  NC_000913/4457181‑4457664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑