Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,154,691	G→A	Q53* (CAG→TAG)	argE ←	acetylornithine deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,154,691	0	G	A	100.0%	117.8 / NA	34	Q53* (CAG→TAG)	argE	acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

TTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTGCACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTG  >  NC_000913/4154455‑4154919                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     tttCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTg                                                                                                                                                                                                                         >  2:91861/1‑250 (MQ=255)  ttCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGa                                                                                                                                                                                                                        >  1:32310/1‑250 (MQ=255)             gACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCtt                                                                                                                                                                                                            >  2:108351/1‑251 (MQ=255)                                            aaaaagctttgaaggTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGGGTAAACGCATAGCGCGTCC‑AGCGACCGT‑CATAAAAGGGCACCGTATCGGTATTCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATGGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATc                                                                                                                                                                             <  1:327959/237‑1 (MQ=255)                                                 gCCTTTCATGTAGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATAGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCAGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCAAATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCAt                                                                                                                                                                        >  1:358846/1‑251 (MQ=255)                                                       cATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCCGCATATTGAATTTGTTGCGAGTTCCTGGAACCGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAACAGAGTGATTAAATCTGCCTTGCttt                                                                                                                                                                  >  2:7370/1‑251 (MQ=255)                                                          ggcggcggTGCCTAAGCCGTAAAGCGTGCGTTCATGCCCCTGCAGTGTAAACGGATCTC‑TGTCCCAGCGACCGT‑CATCAAAGGACACAGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAATAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGa                                                                                                                                                                <  2:178059/248‑1 (MQ=255)                                                           gcggcggTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATc                                                                                                                                                              <  1:274807/250‑1 (MQ=255)                                                                     cTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCctct                                                                                                                                                    >  1:280840/1‑251 (MQ=255)                                                                        aGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGGAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCCAATGCCACCGTAGCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGCGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTcc                                                                                                                                                 >  1:15823/1‑251 (MQ=255)                                                                              aaaGCTTGCCGTCATGCTCCGTCCGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCCGATTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGgcg                                                                                                                                           >  2:109603/1‑251 (MQ=255)                                                                                       agtcgtGCTCCGTAAGTGTAAACAGATCGCGCGCCC‑GGCGACGGTACA‑CAACTGGCCCCTTATCGTTATTGCCCGCCAGCAACAAGCCGCCAGCCCCCTTTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACGTCCACATTGAAGCCCAAATCTTTAAACCAGGCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTAATCGAGTGCCTCTTCCGTGGCGCTTATTGaa                                                                                                                                  <  2:76673/246‑1 (MQ=255)                                                                                                      gtgtAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAAGGGCACCGGATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCaga                                                                                                                   <  1:271183/251‑1 (MQ=255)                                                                                                        gtAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAgc                                                                                                                 <  1:313664/251‑1 (MQ=255)                                                                                                                                      caacaAAGGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGACGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTATTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCGCGATAAATGGCGGTAAt                                                                                     <  2:23327/248‑1 (MQ=255)                                                                                                                                       atcaAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAAtt                                                                                    <  1:138295/251‑1 (MQ=255)                                                                                                                                           aaTGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGCATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGCGCGCGGTAAATCTCGATAAATGGCGGTAATTTGtt                                                                                >  2:216952/1‑251 (MQ=255)                                                                                                                                             tGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAACTCTCGATAAATGGCGGTAATTTGtttt                                                                              >  2:289483/1‑251 (MQ=255)                                                                                                                                                           tATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGacac                                                                 >  1:169955/1‑250 (MQ=255)                                                                                                                                                              tccccGCCAGCAACAATCCGCCAGCCCCCTTGCCGATACTTGCCAGCATATTGAATTTGTTGCTAGTTCCTGGAACAGGCTACACTTCCACATGGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGGTGTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGATAAATCTCTTTAAATGTCGGGAAGTTGTTTTTCATTGTTGaaacaca                                                              <  2:152611/249‑2 (MQ=255)                                                                                                                                                                            aaGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACCCACCTCTGGTCATGAt                                                  >  2:51167/1‑248 (MQ=255)                                                                                                                                                                                  ccAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAat                                         <  2:78878/251‑1 (MQ=255)                                                                                                                                                                                            gTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTACCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGc                                 <  1:126887/249‑1 (MQ=255)                                                                                                                                                                                              ccGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTACTTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGt                              >  1:354602/1‑250 (MQ=255)                                                                                                                                                                                              ccGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACGCCTCTGGTCATGATAGTATCAATATTCATGCAGt                              <  1:179342/250‑1 (MQ=255)                                                                                                                                                                                                gATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTAtt                           <  2:111832/251‑1 (MQ=255)                                                                                                                                                                                                      tGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATg                       <  1:46796/249‑1 (MQ=255)                                                                                                                                                                                                               atTGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATac            >  1:132351/1‑251 (MQ=255)                                                                                                                                                                                                                 tGAATTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATaca           <  1:85476/250‑1 (MQ=255)                                                                                                                                                                                                                    aTTTGTTGCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGACGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCCTTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTa        >  2:1765/1‑250 (MQ=255)                                                                                                                                                                                                                        gttgCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGtt   <  2:32310/251‑1 (MQ=255)                                                                                                                                                                                                                        gttgCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGtt   <  2:91046/251‑1 (MQ=255)                                                                                                                                                                                                                        gttgCGAGTTCCTGGAACAGGCTACACTTCCACATGGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCAGTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGtt   <  2:315523/251‑1 (MQ=255)                                                                                                                                                                                                                          tgCGAGTTCCTGGAACAGGCTACACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTg  <  1:108351/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     TTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCC‑AGCGACCGT‑CATCAAATGGCACCGTATCGGTATGCCCCGCCAGCAACAAGCCGCCAGCCCCCTGTCCGATACTTGCCAGCATATTGAATTTGTTGCGAGTTCCTGGAACAGGCTGCACTTCCACATTGAAGCCCAAATCTTTAAACCAGTCCGCCAGCAGAGTGATTAAATCTGCATTGCTTTGATCGAGTGCCTCTTCCGTGGCGCTTATTGAAGGTGTGGCAATCAGAGCGCGGTAAATCTCGATAAATGGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTG  >  NC_000913/4154455‑4154919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑