Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,042,171	G→A	intergenic (+146/‑197)	zinT → / → yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,042,171	0	G	A	100.0%	71.1 / NA	20	intergenic (+146/‑197)	zinT/yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

ACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAAC  >  NC_000913/2041929‑2042411                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 acaGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAAcc                                                                                                                                                                                                                                          <  2:111441/251‑1 (MQ=255)              ttaAATGAACTGGACCACTGGCAAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTcc                                                                                                                                                                                                                             >  1:294740/1‑251 (MQ=255)               taAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTcc                                                                                                                                                                                                                             >  1:343749/1‑250 (MQ=255)                                                            gTAGCAAAGAAGGGGTCGAGGAAATGATGTCTAATTGAGTATTCTAATGATAACGCCTCGAGGCCGCTTTAGTAAGTTATCAAAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCAtt                                                                                                                                                                               <  2:191797/251‑1 (MQ=255)                                                                 gaagaaGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCtt                                                                                                                                                                           >  1:167912/1‑250 (MQ=255)                                                                     aaatGGTCGAGAAAATGATGTCTCATTGAGTATTCTCATGATACCGCCCCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACGCAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATAtt                                                                                                                                                                      <  1:110777/248‑1 (MQ=255)                                                                       gTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTgg                                                                                                                                                                    <  2:24583/251‑1 (MQ=255)                                                                             gAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGtt                                                                                                                                                              <  1:52039/251‑1 (MQ=255)                                                                                   atgatgTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAata                                                                                                                                                        <  2:306910/251‑1 (MQ=255)                                                                                                 aGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAAttt                                                                                                                                          <  1:308676/251‑1 (MQ=255)                                                                                                         cATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAAt                                                                                                                                   <  2:10780/250‑1 (MQ=255)                                                                                                                aCGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAat                                                                                                                                                         >  1:287980/1‑221 (MQ=255)                                                                                                                aCGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAat                                                                                                                                                         <  2:287980/221‑1 (MQ=255)                                                                                                                      cGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGAGACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTaaa                                                                                                                     <  2:183316/251‑1 (MQ=255)                                                                                                                       gATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTaaa                                                                                                                     >  1:320094/1‑250 (MQ=255)                                                                                                                        aTGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAAc                                                                                                                   >  2:11148/1‑251 (MQ=255)                                                                                                                                                              atccaCAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAg                                                                             <  1:281633/251‑1 (MQ=255)                                                                                                                                                                    aaaagCCACTGAACGCAAGCTAGCTACAGACAAGCTCATCAATATGACGTGTCTGAATATTAATAAGCTAACACGCATTAAGTTAACCAATAAAGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGGTAAAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTACGTGGGATAGCtacac                                                                       >  2:81613/5‑247 (MQ=255)                                                                                                                                                                                                                 gACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAAc                          >  1:341733/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaCCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAAc  >  1:147725/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAAC  >  NC_000913/2041929‑2042411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑