Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,220,685	A→G	F601F (TTT→TTC)	arpA ←	regulator of acetyl CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,220,685	0	A	G	100.0%	63.1 / NA	17	F601F (TTT→TTC)	arpA	regulator of acetyl CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/5);  total (12/5)

CCGGTGACCAAATTCAAAAGCCAACTCAAAGGCAGAGTATTTTTGTGGGGCTTTGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATC  >  NC_000913/4220439‑4220921                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             ccGGTGACCAAATTCAAAAGCCAACTCAAAGGCAGAGTATTTTTGTGGGGCTTTGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACa                                                                                                                                                                                                                                          <  1:293413/251‑1 (MQ=255)   ggTGACCAAATTCAAAAGCCAACTCAAAGGCAGAGTATTTTTGTGGGGCTTTGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACaga                                                                                                                                                                                                                                        <  1:202436/251‑1 (MQ=255)   ggTGACCAAATTCAAAAGCCAACTCAAAGGCAGAGTATTTTTGTGAGGCATTGTGTTGCCAAAAATCCATAATATATTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAACGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAAc                                                                                                                                                                                                                                           >  1:56609/1‑248 (MQ=255)                                                    tttGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACCGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGGGTCCGCGCt                                                                                                                                                                                       >  1:326348/1‑251 (MQ=255)                                                     ttGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCt                                                                                                                                                                                       <  1:232592/250‑1 (MQ=255)                                                      tgtgTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACAGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGc                                                                                                                                                                                     >  2:359582/1‑251 (MQ=255)                                                        tgtTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCAt                                                                                                                                                                                   >  1:48052/1‑251 (MQ=255)                                                                                                                                                 ttgttgTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCt                                                                                           <  2:200442/250‑1 (MQ=255)                                                                                                                                                     tgttTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCt                                                                                      >  1:309785/1‑251 (MQ=255)                                                                                                                                                      gttTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCt                                                                                      <  1:250679/250‑1 (MQ=255)                                                                                                                                                             cTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATg                                                                              >  1:36198/1‑251 (MQ=255)                                                                                                                                                                     cTGAAAACAAACCGGGATATTAATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTTATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCa                                                                         >  2:255333/1‑248 (MQ=255)                                                                                                                                                                                            ttAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATCTCGCTATg                                               >  2:119356/1‑251 (MQ=255)                                                                                                                                                                                                                   gggCTTCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGatat                         >  1:163931/1‑250 (MQ=255)                                                                                                                                                                                                                       ttCATATTTTTTTTATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTcc                    >  1:87547/1‑251 (MQ=255)                                                                                                                                                                                                                                 ttttATCGAATTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAAcc           >  1:369935/1‑250 (MQ=255)                                                                                                                                                                                                                                         aaTTTAAACGTATTGAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATc  >  2:296425/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             CCGGTGACCAAATTCAAAAGCCAACTCAAAGGCAGAGTATTTTTGTGGGGCTTTGTGTTGCCAAAAATCCATAATATCTTCAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATC  >  NC_000913/4220439‑4220921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑