Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,100,227	G→A	S100L (TCG→TTG)	rhaT ←	rhamnose/lyxose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,100,227	0	G	A	100.0%	156.2 / NA	42	S100L (TCG→TTG)	rhaT	rhamnose/lyxose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/23);  total (19/23)

CCGCACATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCGACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATGGTTTCCCATGACCATTTTTTTACTTTTTTGAACGGAGC  >  NC_000913/4099981‑4100456                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      ccGCACATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACAt                                                                                                                                                                                                                                   >  2:221669/1‑251 (MQ=255)  cGCACATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCCTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATg                                                                                                                                                                                                                                  >  1:333147/1‑251 (MQ=255)   gCACATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAAGTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGc                                                                                                                                                                                                                                 <  1:221671/251‑1 (MQ=255)                aGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCAt                                                                                                                                                                                                                    <  1:248789/251‑1 (MQ=255)                   aCCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGt                                                                                                                                                                                                                 >  1:271580/1‑251 (MQ=255)                           ttttttCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGt                                                                                                                                                                                                         >  2:103552/1‑251 (MQ=255)                             ttttCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGt                                                                                                                                                                                                         >  1:152192/1‑249 (MQ=255)                              tttCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGt                                                                                                                                                                                                      >  2:309402/1‑251 (MQ=255)                               ttCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGt                                                                                                                                                                                                      <  1:221669/250‑1 (MQ=255)                                      tGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCAACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATAAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATAtt                                                                                                                                                                                               >  1:314605/1‑250 (MQ=255)                                            ctTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATc                                                                                                                                                                                        >  1:155749/1‑251 (MQ=255)                                             ttCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATcc                                                                                                                                                                                       >  2:171963/1‑251 (MQ=255)                                              tCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGAt                                                                                                                                                                                         <  1:88518/248‑1 (MQ=255)                                                   tttAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCcac                                                                                                                                                                                  >  1:326836/1‑250 (MQ=255)                                                      aaTGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATa                                                                                                                                                                               <  1:112648/250‑1 (MQ=255)                                                                            aaCTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCCATCCCCCACATAACGCCGAACAGAAAAACAGGc                                                                                                                                                          >  2:182417/1‑249 (MQ=255)                                                                                gCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTcgc                                                                                                                                                    >  1:67322/1‑251 (MQ=255)                                                                                         gAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAgagaga                                                                                                                                           <  1:235023/251‑1 (MQ=255)                                                                                          aggtACAATCAATACGCCTATCAGCGCCACCAGAAAGCCGAGCAATGTCATGCGTCCGCACTCGGTGCTAATCAACACATCGAAAGTGCCCGTGATAATTGGCGTCATCTGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCCACAACATGCCGAGATAACGCACGGTCAGGCCGTAGTTGATATTACCGATCACCCACACAGCGCCGAACAGAAAAGCAGTCATTCTCTTAGAGAGAc                                                                                                                                          <  2:192193/248‑1 (MQ=255)                                                                                               cAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAAGTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAAc                                                                                                                                     <  2:282822/251‑1 (MQ=255)                                                                                                         gCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAAt                                                                                                                           >  1:109511/1‑251 (MQ=255)                                                                                                            aaTCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACg                                                                                                                        <  2:326836/251‑1 (MQ=255)                                                                                                                                   gCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAAGACGCCCAGAAATTCGGTAGTAACAgg                                                                                                 <  1:300065/251‑1 (MQ=255)                                                                                                                                    cAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAggg                                                                                                >  1:161756/1‑251 (MQ=255)                                                                                                                                      aCGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATGGGCGTCATCAGCGTACCGACAATCAACGTAATTCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAAGTCGGTAGTAACAGGgcg                                                                                              <  2:333147/251‑1 (MQ=255)                                                                                                                                                 ccGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGccc                                                                                    >  1:225080/1‑250 (MQ=255)                                                                                                                                                  cGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAATAGGCAGTCGCGTAGAGAGACGAAGCGAGCTGTAAGACGCCCAGAAATGCGGTAGTAGCAGGGCGCTGATGGCCCAc                                                                                  <  2:184823/251‑1 (MQ=255)                                                                                                                                                        cGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAg                                                                             <  1:350993/250‑1 (MQ=255)                                                                                                                                                                      acaTCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAAc                                                              <  1:219225/251‑1 (MQ=255)                                                                                                                                                                                           aTTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCa                                                                                                                                                         <  1:325244/139‑1 (MQ=255)                                                                                                                                                                                           aTTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCa                                                                                                                                                         >  2:325244/1‑139 (MQ=255)                                                                                                                                                                                           aTTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACAt                                         <  2:302615/251‑1 (MQ=255)                                                                                                                                                                                             tGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATg                                        <  2:155749/250‑1 (MQ=255)                                                                                                                                                                                              ggCGTCAGCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATGCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTGCCGATCCCCCACATAGCTCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAATCGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCAGGAAACaagccgtgcgactttccacatggt                                      <  2:345686/251‑23 (MQ=255)                                                                                                                                                                                                  tcatcaGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATGGTTTcc                                  >  2:365423/1‑251 (MQ=255)                                                                                                                                                                                                   catcaGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATGGTTTcc                                  <  2:67322/250‑1 (MQ=255)                                                                                                                                                                                                    atcaGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCATAATAATCCACGAAACAATCCCACCGACTGACCACATGGTTTCCCa                                <  1:334699/251‑1 (MQ=255)                                                                                                                                                                                                              cGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATGGttt                                    >  1:265562/1‑237 (MQ=255)                                                                                                                                                                                                              cGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATGGttt                                    <  2:265562/237‑1 (MQ=255)                                                                                                                                                                                                                            aTGCCAATGGCGATGCCAATGCCCATCAACATGCCGAGATAACGCATTGTCAGGCCGTATTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAACCCCGCCGACTGACCACATGGTGTCCCATGACCATGTTTTTACTGTTTTGaa        <  2:360466/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaTGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATGGTTTCCCATGACCATTTTTTTACTTTTTTGAACgg     >  2:295600/1‑249 (MQ=255)                                                                                                                                                                                                                                  aTGGCGATGCCAATTCCCATCAACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATGGTTTCCCATGACCATTTTTTTACTTTTTTGAACGGAga  <  2:121549/251‑2 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      CCGCACATCACCGCCAGCACCAGCCCTTTTTTCAGATTGAACTCTTCGGCTTTAATGCCCATCTTGCGCTCTTTCAACTGCCCGGCGCGAGTTACAATCCCTACGCCAATCAGCGCCACCAGAACGCCGAGCAACGTCATGCGTCCGCCTTCGGTGCTAATCAACACATCGAAATTGCCGTTGATAATTGGCGTCATCAGCGTACCGACAATCAACGTAATGCCAATGGCGATGCCAATTCCCATCGACATGCCGAGATAACGCATGGTCAGGCCGTAGTTGATATTACCGATCCCCCACATAGCGCCGAACAGAAAAACAGGCAGTCGCGTAGAGAGACTAAACGAGCTGTAATACGCCCAGAAATTCGGTAGTAACAGGGCGCTGATGGCCCACGGCAGAATAATCCACGAAACAATCCCACCGACTGACCACATGGTTTCCCATGACCATTTTTTTACTTTTTTGAACGGAGC  >  NC_000913/4099981‑4100456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑