Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,465,502	G→A	E141K (GAG→AAG)	gspM →	Type II secretion system protein GspM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,465,502	0	G	A	100.0%	93.4 / NA	27	E141K (GAG→AAG)	gspM	Type II secretion system protein GspM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/10);  total (17/10)

ACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCT  >  NC_000913/3465271‑3465731                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       aCCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTTTTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCAttt                                                                                                                                                                                                                     >  1:35857/1‑251 (MQ=255) aCCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCAttt                                                                                                                                                                                                                     <  1:125857/251‑1 (MQ=255)           tACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAgg                                                                                                                                                                                                           >  1:146519/1‑251 (MQ=255)                                    gCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACt                                                                                                                                                                                  <  1:13447/251‑1 (MQ=255)                                      ccATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCCGGGAGCAACAATAATGACAATGCTACTGc                                                                                                                                                                                >  2:297854/1‑251 (MQ=255)                                                tatCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCAt                                                                                                                                                                      >  1:205610/1‑251 (MQ=255)                                                                     aCGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGc                                                                                                                                                    >  1:361054/1‑248 (MQ=255)                                                                     aCGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGa                                                                                                                                                 <  1:62616/251‑1 (MQ=255)                                                                      cGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTTTCCTTCAGGGAGCAACAATAATGACACTGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGc                                                                                                                                                    >  2:104223/1‑247 (MQ=255)                                                                                  gCTATCACGCTGGAAAACGGCCCGGACCATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCACGCTGGCTCACTACGGCACCGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCCTTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAAt                                                                                                                                    >  2:150956/1‑251 (MQ=255)                                                                                     aTCACGCTGGAAAACGGCCCGGACAATACTCTGACCATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGcc                                                                                                                                 >  2:289571/1‑251 (MQ=255)                                                                                                  aCGGCACGGACAATACTCTGACAATTCCTCCTGTCACCGCTCCCCTCGAACACGTCTCCCGCTGGCTCACTACGGCACAGGCAACATACGGTATTGTTATTGAAGACCTTCAGTTTACACTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCACCAATAATGACAATGCTACCGCCGCTCTTCCTTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCAcc                                                                                                                    >  2:329830/1‑251 (MQ=255)                                                                                                      cccGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCtctc                                                                                                                >  1:93986/1‑251 (MQ=255)                                                                                                                        aaTTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAAc                                                                                              <  1:150866/251‑1 (MQ=255)                                                                                                                         aTTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACg                                                                                             <  2:253629/251‑1 (MQ=255)                                                                                                                                 tGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACAGCACAGGTAAAATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAAc                                                                                     >  1:247125/1‑251 (MQ=255)                                                                                                                                          tCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGcc                                                                             >  1:263325/1‑250 (MQ=255)                                                                                                                                                                     cACTACGGCACAGGTAGCATGCGGTCTTGGTATTGAAGACCTTCAGTTTACTCTTGCAGGAAAGCAAAAAATAACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATACCACGACGTTAACGTTTCGCCCGGTCTTGGTTCATTTCAGGCacaaca                                                  >  2:205684/1‑245 (MQ=255)                                                                                                                                                                                  gTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGCAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCAt                                   >  1:22460/1‑251 (MQ=255)                                                                                                                                                                                    aaCATACGGTATTGTTATTGAAGACCTTCAGTTTCCTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATg                                  <  2:191369/250‑1 (MQ=255)                                                                                                                                                                                             tATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGGATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATGATACAGTGcc                        >  1:12647/1‑251 (MQ=255)                                                                                                                                                                                              aTTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATGATACAGTGcc                        <  2:326104/250‑1 (MQ=255)                                                                                                                                                                                                tGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATGATACAGTGcccc                      <  1:340837/250‑1 (MQ=255)                                                                                                                                                                                                  ttATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATTACCCCTGGCATGaaccgggccccctg                    >  1:113906/1‑238 (MQ=255)                                                                                                                                                                                                  ttATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATGATACAGTGCCCCTg                    >  1:331154/1‑250 (MQ=255)                                                                                                                                                                                                          gACCTTCAGTTTACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATGATACAGTGCCCCTGATACTTtgt           <  2:172655/251‑1 (MQ=255)                                                                                                                                                                                                                    ttACTCTTGCCGGAAACGAAAAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgctgct  <  1:236868/250‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       ACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAAT‑ATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCT  >  NC_000913/3465271‑3465731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑