Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,101,785	G→A	A32A (GCG→GCA)	kdgT →	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,101,785	0	G	A	100.0%	79.8 / NA	26	A32A (GCG→GCA)	kdgT	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/11);  total (15/11)

AAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGC  >  NC_000913/4101550‑4102027                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   aaataaaaCAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTgg                                                                                                                                                                                                                                      >  1:298928/1‑250 (MQ=255)            cGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACTCCTTCTCTCCGGGGTCAGGGAAATATTTTGGATCATTCACCaa                                                                                                                                                                                                                          <  1:129614/251‑1 (MQ=255)             gTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAata                                                                                                                                                                                                                                            <  2:353859/232‑1 (MQ=255)             gTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAata                                                                                                                                                                                                                                            >  1:353859/1‑232 (MQ=255)               ttCAATTGATGCGGTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTAG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCACCgg                                                                                                                                                                                                                       >  1:106795/1‑251 (MQ=255)                 cAATTGATGTGGTTTTGATCACTTTTATTGATTACA‑TGAATGCCTATCTTCGTTTCCCTCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATACCGGGGGG‑ATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGCAATATTTTGGATCATTCACCACCGGgat                                                                                                                                                                                                                    >  1:171191/1‑251 (MQ=255)                  aaTTGAGGGGGTTTTGATCCCTTTTAT‑GATTAAAATGAAGTTCTATTTCCGTTCCAACCACCACTGATCCCCAATTAAGGATTTAC‑CATTAACCCTTAAAAAAAATCCCACCAGTTGATGGAAATGCA‑ATAAAACCCCCGGTTTAAGAAAACCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGgat                                                                                                                                                                                                                    <  1:302274/251‑1 (MQ=255)                        tgtgGTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTa                                                                                                                                                                                                               >  2:3699/1‑250 (MQ=255)                           ggTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCgg                                                                                                                                                                                                           >  2:141891/1‑251 (MQ=255)                                        tttATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCAt                                                                                                                                                                                               <  2:158276/250‑1 (MQ=255)                                                   aa‑tgaatgTCTGTATACGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGCCAGGGGATGGAACTGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTACTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGAACATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCggcttg                                                                                                                                                                                   >  2:211009/1‑247 (MQ=255)                                                      tgaatgTCTATCTTCGTTTCCATCAACACTGCTGCTCCCTTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAACACGCTCG‑ATTGAGAAAATCCCGGGGGGGACGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGTGATGATTACCGGTACGGTGCCCATTCTTGCGGTGTGGt                                                                                                                                                                                 >  2:208050/1‑251 (MQ=255)                                                                                                aGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCACCGGGATGATTACCGGTACGGTGCCCATTCTGTCGGTGTGGTTTTTTTGCATGGGGGCGTCAATATAATTAAGCTCGACGGGa                                                                                                                                        >  2:34661/1‑250 (MQ=255)                                                                                                        cGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTg                                                                                                                               <  2:334967/251‑1 (MQ=255)                                                                                                        cGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAAGCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTTAGCGCGACGGGAACGGTACTg                                                                                                                               <  2:334941/251‑1 (MQ=255)                                                                                                                      aaaaTA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTAc                                                                                                                 <  1:156447/251‑1 (MQ=255)                                                                                                                         ata‑tGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATATTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTCCGCt                                                                                                              <  2:256606/251‑1 (MQ=255)                                                                                                                              gCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCtggtg                                                                                                          >  2:95736/1‑251 (MQ=255)                                                                                                                               ccGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCtggtgg                                                                                                         >  2:360296/1‑251 (MQ=255)                                                                                                                                       tGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTaa                                                                                                  >  1:12932/1‑250 (MQ=255)                                                                                                                                                                     gagaAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAt                                                                    <  1:99097/251‑1 (MQ=255)                                                                                                                                                                                                gTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGGGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAATCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTTCCGTCGCGTGGGTGGTTGCTGCAATTGCCTCGCGTATTATTCCGGAACATgg                                         <  1:211640/251‑1 (MQ=255)                                                                                                                                                                                                        aTTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAg                                 <  1:346002/251‑1 (MQ=255)                                                                                                                                                                                                               ggCGCACTGTGCCACACCTTCTCGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGAtt                          >  2:88693/1‑251 (MQ=255)                                                                                                                                                                                                                                  tctcGCCGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTTCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAcg       >  1:219267/1‑251 (MQ=255)                                                                                                                                                                                                                                       ccGGGGGCAGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGAGGTGTGGTTTTTTAGCATGGGGGAGTCCATAAAATTCAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGGGGTTGCGGCAATTGCCTCGCGTATTATTCCGGCACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAcgctggc  >  1:359431/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   AAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAA‑TGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATA‑TGCCGACAGGTGATGGAAATGCAGATAAAACGCTCG‑ATTGAGAAAATCCCGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGC  >  NC_000913/4101550‑4102027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑