Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,192,667	G→A	I57I (ATC→ATT)	thiS ←	sulfur carrier protein ThiS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,192,667	0	G	A	100.0%	111.5 / NA	31	I57I (ATC→ATT)	thiS	sulfur carrier protein ThiS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/16);  total (15/16)

GCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGGATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGA  >  NC_000913/4192437‑4192912                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      gccgctgtgtcgcctccgccgCGATAAGCCGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGGTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCCGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTTAATCAACCTTTTGGTCCGCAATACGTAACATTTCATCCCCCTGCAATAACCTGAAAAAGCAGAATCTTGTCGGCATTCTGCAc                                                                                                                                                                                                                                   <  2:234195/236‑1 (MQ=255)         cgcccccccGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGtt                                                                                                                                                                                                                           <  2:10825/245‑1 (MQ=255)              ccccGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGcc                                                                                                                                                                                                                      <  1:369653/250‑1 (MQ=255)              acCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAAAGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGcc                                                                                                                                                                                                                      >  2:359611/1‑251 (MQ=255)                                                  gtgCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCATCCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGcc                                                                                                                                                                                  >  2:284818/1‑251 (MQ=255)                                                         cgcAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCcg                                                                                                                                                                           <  1:112569/251‑1 (MQ=255)                                                                     cGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTc                                                                                                                                                               <  1:299692/251‑1 (MQ=255)                                                                      gTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCg                                                                                                                                                              >  2:206310/1‑251 (MQ=255)                                                                       tttCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCCTCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGt                                                                                                                                                             >  2:152542/1‑251 (MQ=255)                                                                              gCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCg                                                                                                                                                       >  2:349566/1‑250 (MQ=255)                                                                                       aCCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTcc                                                                                                                                              >  2:64986/1‑250 (MQ=255)                                                                                                      gAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGGTCGTGAACAg                                                                                                                              <  1:66569/251‑1 (MQ=255)                                                                                                        aGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGACTTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGtt                                                                                                                            >  2:230879/1‑251 (MQ=255)                                                                                                                        accaTAAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACt                                                                                                            >  2:212266/1‑251 (MQ=255)                                                                                                                             catttGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATc                                                                                                       <  2:294268/248‑1 (MQ=255)                                                                                                                             cAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCAt                                                                                                        <  1:327036/250‑1 (MQ=255)                                                                                                                                          gCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTa                                                                                           >  1:247488/1‑250 (MQ=255)                                                                                                                                                        gCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCAt                                                                             <  1:219746/250‑1 (MQ=255)                                                                                                                                                            gTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCtt                                                                        >  1:270818/1‑251 (MQ=255)                                                                                                                                                                aCAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTtcctc                                                                    >  2:352336/1‑251 (MQ=255)                                                                                                                                                                        gAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATAcc                                                            >  2:130773/1‑251 (MQ=255)                                                                                                                                                                        gAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATAc                                                             <  1:365176/250‑1 (MQ=255)                                                                                                                                                                                                      aTTTCAACCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGATCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTCCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAgg                              >  2:351503/1‑251 (MQ=255)                                                                                                                                                                                                            aCCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGt                                                                                                                                                    >  1:158738/1‑127 (MQ=255)                                                                                                                                                                                                            aCCCCCTGCAATAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACGGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGt                                                                                                                                                    <  2:158738/127‑1 (MQ=255)                                                                                                                                                                                                                       tAACCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCg              >  1:269620/1‑250 (MQ=255)                                                                                                                                                                                                                        aaCCTGAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGAt            <  1:1443/251‑1 (MQ=255)                                                                                                                                                                                                                             gAAAAAGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTAc        <  1:104013/250‑1 (MQ=255)                                                                                                                                                                                                                              aaaaaGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCg      <  2:269620/251‑1 (MQ=255)                                                                                                                                                                                                                               aaaaGCAGAATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGt     <  1:217374/251‑1 (MQ=255)                                                                                                                                                                                                                                  aGCAGAATCGGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTGGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGa  <  1:252680/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      GCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGGATCTGGTCGCCATCCTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGA  >  NC_000913/4192437‑4192912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑