Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,080,078	G→A	intergenic (+109/+221)	yiiF → / ← fdhE	protein YiiF/formate dehydrogenase formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,080,078	0	G	A	100.0%	84.7 / NA	25	intergenic (+109/+221)	yiiF/fdhE	protein YiiF/formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/12);  total (13/12)

GACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATACGAGAGATGGTGTCGTCACACCCTATGAGCTGTAATCCGGGCGACCTGCGCCACCCGGAAAACGTCAGAGATTACTCTCCTTCA  >  NC_000913/4079835‑4080311                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          gACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGttt                                                                                                                                                                                                                                     >  1:350652/1‑250 (MQ=255)      aTTAAGGGAAGCGGTAGATCAATACCTGATAACACAATCGCAAACCGCAAGAAACAGTGTTCCTGGCATCAGGCAAGGGTGTAAGGAACATGGTGTCGAATATCAGCGTAAGCTCCGCGAGGCATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGAGGCGTGAACGCCTTATCCGCCATACAAAGTCGTATACATTACATGTATTACTGGTTATTGGt                                                                                                                                                                                                                               >  1:173179/1‑251 (MQ=255)                   gTAGATCAATACCTGATAAATCAATCGCAAACAGCAATAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAagc                                                                                                                                                                                                                  <  1:73447/251‑1 (MQ=255)                         cAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAagcgcagcg                                                                                                                                                                                                            <  2:26402/251‑1 (MQ=255)                             aCCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATc                                                                                                                                                                                                        >  1:239809/1‑251 (MQ=255)                                             gCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCtt                                                                                                                                                                                        <  1:144456/251‑1 (MQ=255)                                                                          tCTGGCAAGGGGGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAtt                                                                                                                                                           >  1:272217/1‑251 (MQ=255)                                                                             ggCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAt                                                                                                                                                        <  2:272217/251‑1 (MQ=255)                                                                              gCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtg                                                                                                                                                       <  2:343279/251‑1 (MQ=255)                                                                               cAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtgt                                                                                                                                                      >  2:111377/1‑251 (MQ=255)                                                                                        gAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGtggtggtgg                                                                                                                                              <  1:72204/250‑1 (MQ=255)                                                                                          ggAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTa                                                                                                                                           <  2:205757/251‑1 (MQ=255)                                                                                                                cGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCAGCAGTCTCAGGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAATCCGGCACTg                                                                                                                     <  2:144661/251‑1 (MQ=255)                                                                                                                         cgcgAGGAATGGTAATGGGAAAAAATGATGTCACTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCAt                                                                                                              >  2:26892/1‑249 (MQ=255)                                                                                                                                   ggTAATGGGAAAAAATAATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGACGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGCATAAATTCACTGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGTCAAATCCAGGCCAAACCGGCACCGGCGGCATAGCTTgcgag                                                                                                    >  2:259921/1‑245 (MQ=38)                                                                                                                                      aaTGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTaa                                                                                               <  1:148179/251‑1 (MQ=255)                                                                                                                                                aaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCAAAGTCGTATAAATTCAATGTATTACCGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGGAACGCGGAATAc                                                                                     >  2:37390/1‑251 (MQ=255)                                                                                                                                                        cAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGCGGTAACGCGGAATACGAGAGATg                                                                             >  1:248952/1‑251 (MQ=255)                                                                                                                                                          aTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATACGAGAGATGgt                                                                           <  2:70319/251‑1 (MQ=255)                                                                                                                                                                                 gttgttCATGCCTGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAAGTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTTGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATGCGAGAGATGGTGTCGTCACACCCTATggtctgta                                                    <  2:146307/251‑8 (MQ=255)                                                                                                                                                                                  ttgttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATACGAGAGATGGTGTCGTCACACCCTATGAGCTGTa                                                    >  2:40198/1‑250 (MQ=255)                                                                                                                                                                                                          aCGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATACGAGAGATGGTGTCGTCACACCCTATGAGCTGTAATCCGGGCGACCTGCGCCACCCgg                            >  2:194665/1‑250 (MQ=255)                                                                                                                                                                                                             ccTTATCCGGCCTACAACGTCGTATAAATTCCATGCATTACAGTTTATTGGTAGGCCTGCTAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATACGAGAGATGGTGTCGTCACACCCTATGAGCTGTAATCCGGGCGACCTGCGCCACCCGGaaa                         >  2:332131/1‑250 (MQ=255)                                                                                                                                                                                                                              aGTCGTATAAATTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATACGAGAGATGGTGTCGTCACACCCTATGAGCTGTAATCCGGGCGACCTGCGCCACCCGGAAAACGTCAGAGATTACtctc       >  1:129259/1‑251 (MQ=255)                                                                                                                                                                                                                                    ataAAGTCAATGTATTACAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATACGAGAGATGGTGTCGTCACACCCTATGAGCTGTAATCCGGGCGACCTGCGCCACCCGGAAAACGTCAGAGATTACTCTCCTTCa  <  1:157486/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          GACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGCCAAACCGGCACTGGCGGCATAGCTTGAGGTGGTAACGCGGAATACGAGAGATGGTGTCGTCACACCCTATGAGCTGTAATCCGGGCGACCTGCGCCACCCGGAAAACGTCAGAGATTACTCTCCTTCA  >  NC_000913/4079835‑4080311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑