Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	109.5 / NA	33	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/16);  total (17/16)

ATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATG  >  NC_000913/696258‑696706                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             atatGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                                                                                                                                                                                                        >  1:64517/1‑251 (MQ=255)   atGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCATCTggg                                                                                                                                                                                                      >  2:23538/1‑251 (MQ=255)          aGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt                                                                                                                                                                                               <  1:179004/251‑1 (MQ=255)              ttGTAGCCTTACAACATTGCCGGATGCGTGACAACCGTCGCGAGGAAAAGACGCGGACGCGTAGCCTCCAACCCTGAAGGA‑CAGCAGTGT‑AACAATCGCATCCGAAATCGAATGAACGAAGACAAGCAAAAAGCTCGCCGCAGCGAGCTTTTTTAATGTGGATGGGGTAAGAGGCTTCGAAACTAGGAATGCCGGAATTAGAATCCGGCACCTTACAGCTTGGCGATACCCCAACTggtaaaagctacact                                                                                                                                                                                          >  1:166565/1‑238 (MQ=255)                    ccTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGCTAAGACGCGGCAGCGTCGCATCCGACATTGAAGGCTAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGCTGAACTTTCAAGGTa                                                                                                                                                                                       >  2:228518/1‑249 (MQ=255)                    ccTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAg                                                                                                                                                                                     <  1:170151/251‑1 (MQ=255)                           acaTTGCCGGATGCGTGCCACACGTAGGTCGGATAAGCCGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGTGAGCTTTTTTAATGTGGCTGTGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAATGTAAGCGTCTTg                                                                                                                                                                              <  2:144324/251‑1 (MQ=38)                                   ggATGCGTGCCAACCGTAGGGCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCGGGTGCCTTACCGCTTGGCGATACCCAAACTGGGTGGACTTAAAAGGTAAgggcctggaagaaatt                                                                                                                                                                      >  1:141642/1‑236 (MQ=38)                                                                     gCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                                                                                                                    <  2:80433/251‑1 (MQ=255)                                                                       aGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTTGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCt                                                                                                                                                                                <  2:188166/205‑1 (MQ=255)                                                                       aGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCt                                                                                                                                                                                >  1:188166/1‑205 (MQ=255)                                                                                      aTTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCGGGGGTACGAGGATTCGAAGAGAGGAATGCCGGAATCAGAATCCggct                                                                                                                   >  2:235223/1‑249 (MQ=38)                                                                                                 agaggtgtCAACA‑GCACATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCTAAGCGAGCTATTTTAATGTGGCTGGGGTACGAGGATGCGAACCTCGGAATGACGAAATTAGAATACGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAATTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGTTGCCTTACCGCtt                                                                                                        <  2:154351/247‑1 (MQ=38)                                                                                                  gACGTGGCAGCA‑GCGCTTTCGACATTGAATGAACGCAGACAAGAAAAAAGCTCGCCGAAGCGAGCTTGGTTAATGTGGCTGGGGTACGATGACTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                                                                       <  2:136659/251‑1 (MQ=38)                                                                                                            cA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCATTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTAGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATAAGAAGCCGGTGCCTTACCGCTTGGCGATAccac                                                                                             >  1:172744/1‑249 (MQ=255)                                                                                                                cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAccagatcggaagagcgtcg                                                                                           <  1:339430/250‑18 (MQ=255)                                                                                                                                     gCAGAAAAGTAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTTGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCTACTGGGTGCACGTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATGCGAACCTCGGAATGCCGTAATCAGAATCCTTTGCCTTTCCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCg                                                                     <  1:20922/251‑1 (MQ=38)                                                                                                                                                    gCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGCGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATAc                                                       >  1:227858/1‑250 (MQ=255)                                                                                                                                                            aaGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAAt                                              <  1:95074/251‑1 (MQ=255)                                                                                                                                                              gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAAttt                                            >  1:94954/1‑251 (MQ=255)                                                                                                                                                               cGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGCATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGGATGCCGGAATCAGAATCCGGCGCCTTCCCGCGCGGCGATACCCCCACCAATTGGTTTTGAATTTGCCGCACATAGTCGATAAATTCAGAATTtg                                           >  1:49911/1‑251 (MQ=37)                                                                                                                                                                gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACGCAAGATGGGTGCACTTAGAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATAAGAATCCGGTGCCTTACCGCTTGGc                                                                                                     >  2:155545/1‑192 (MQ=37)                                                                                                                                                                gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                                                                     <  1:155545/192‑1 (MQ=255)                                                                                                                                                                      ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCt                                     <  1:280232/250‑1 (MQ=255)                                                                                                                                                                       tttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGGAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTac                                   >  1:348651/1‑251 (MQ=255)                                                                                                                                                                       ttaaaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGAGTGCACTTACAAGGAAAGCGTCTTGAATAAATTGGCTGGGGTTCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAACTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGCATTTGGTGGCTa                                    >  1:348664/4‑250 (MQ=38)                                                                                                                                                                                   ggggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGa                       <  1:94222/251‑1 (MQ=255)                                                                                                                                                                                      gTACGAGGATTCGAACCTCGGAATGACGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACGTACAAGGTAAGCGTCGTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAAcc                    <  2:124526/251‑1 (MQ=255)                                                                                                                                                                                        acgagtgttCGCACCGCGGAATGCCGGGATTAGAATCCGGGGCCTTACCGCGTGGCGATACCCTAACTGGTTGCACTTACGAGGTAGTCGGCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCTGAATGCCGGAATCAGAATCCGGTGCCTTACCGCGTTGCGATACCCCAACAAATTGGTTTTGAATTTGCCGATCATATTCGATACATTCAGAATTTGGTGTCTACGACGGGATTCGAACCtg                  <  2:237943/244‑1 (MQ=25)                                                                                                                                                                                           aGGATTAGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACACCAACTGGGGGCACTTACAAGGTAAGAGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAGTGGTTTTGAATTTGCAGAACATATTCGATCAATTCAGACTTTGGTGGCTACGACGGAACTCGAACCtgat                >  1:96521/1‑248 (MQ=37)                                                                                                                                                                                                 cGAACCACGGAAGGCCGGAATTAGAATCCGGGGCCTTACCGATTGGCGATACCCCAACTGGGTGCGCTTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTGTTAATGTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGGTTCGAGCCTTTGACCCcat         <  2:331139/251‑1 (MQ=37)                                                                                                                                                                                                   aaCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCcatc        >  1:313704/1‑250 (MQ=255)                                                                                                                                                                                                         ggAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCCGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATg  >  1:173946/1‑250 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             ATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACA‑TCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATG  >  NC_000913/696258‑696706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑