Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,855,402	C→T	A190A (GCG→GCA)	ydjG ←	NADH‑dependent methylglyoxal reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,855,402	0	C	T	100.0%	105.7 / NA	29	A190A (GCG→GCA)	ydjG	NADH‑dependent methylglyoxal reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/17);  total (12/17)

GGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGG  >  NC_000913/1855156‑1855641                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gggAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCt                                                                                                                                                                                                                                             <  2:172574/251‑1 (MQ=255)   gAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCCGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTg                                                                                                                                                                                                                                            >  2:134677/1‑250 (MQ=255)                    cGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCAcc                                                                                                                                                                                                                          <  2:337540/251‑1 (MQ=255)                     gAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCCGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCAcc                                                                                                                                                                                                                          >  2:316217/1‑250 (MQ=255)                           aaaGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGc                                                                                                                                                                                                                   >  1:277245/1‑251 (MQ=255)                            aaGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCa                                                                                                                                                                                                                  <  2:114753/251‑1 (MQ=255)                               tGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGc                                                                                                                                                                                                                   >  2:300587/1‑247 (MQ=255)                                          gTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGata                                                                                                                                                                                                    <  1:255647/251‑1 (MQ=255)                                                      caatcaCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATc                                                                                                                                                                                         <  1:291218/250‑1 (MQ=255)                                                            cTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGtt                                                                                                                                                                                   >  2:359377/1‑250 (MQ=255)                                                              ttCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTagc                                                                                                                                                                                >  2:13624/1‑251 (MQ=255)                                                                 aGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTagcagc                                                                                                                                                                             <  1:151922/251‑1 (MQ=255)                                                                 aGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTagcagc                                                                                                                                                                             <  1:292194/251‑1 (MQ=255)                                                                                     ccAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTcc                                                                                                                                                         <  1:170292/251‑1 (MQ=255)                                                                                            ttATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAg                                                                                                                                                   <  1:200210/250‑1 (MQ=255)                                                                                                           ccgccCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAg                                                                                                                                   <  1:353648/251‑1 (MQ=255)                                                                                                                      ggaagcACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTTAACAACAATGCCATTATCACGACATAGTGGCAGCAGGTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCAACATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGAAGCGCCTATAGCGCGAATTTTCCCGTCAGACTTTAACTCATTAAGCACTTCGACAg                                                                                                                        <  2:327034/246‑1 (MQ=255)                                                                                                                        aaTCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGtt                                                                                                                      >  2:339209/1‑251 (MQ=255)                                                                                                                            aCGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTc                                                                                                                    >  1:231998/1‑249 (MQ=255)                                                                                                                              gAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCg                                                                                                                <  2:346533/251‑1 (MQ=255)                                                                                                                                     ggTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCg                                                                                                         <  2:278942/251‑1 (MQ=255)                                                                                                                                                              aGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGcc                                                                                 >  1:67765/1‑250 (MQ=255)                                                                                                                                                                      aTAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCa                                                                        <  1:237287/251‑1 (MQ=255)                                                                                                                                                                                          cATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATc                                                     >  1:151013/1‑250 (MQ=255)                                                                                                                                                                                                                         ttCCATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTAcc                      <  1:3145/250‑1 (MQ=255)                                                                                                                                                                                                                           ccATTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACctc                    <  1:163021/250‑1 (MQ=255)                                                                                                                                                                                                                             aTTGCCCGGTCGAGGATACTGTATTTTGCCTGAATAATATACCGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGAAGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACctctcc                 >  1:167102/1‑249 (MQ=255)                                                                                                                                                                                                                                          ggATACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCGCGCTGCTACCTCTTCTCGGATAGATTcc    <  2:360024/251‑1 (MQ=255)                                                                                                                                                                                                                                            aTACTGTATTTTGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGACTTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACCTCTTCGCGGATAGATTCCgg  >  1:173116/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGCGGATATGGTCAGCATCGACGTTAGCAGCGCCTATAGCGCGAATTTTCCCTTCAGACTTTAACTCATTAAGCACTGCGACAGTTTCAGCGATCGGCGTAAAAAATGGCGGCACCGACTGCCAGTGCGTCATGTAGATATCGATGTAATCAATACCCAGACGTTGCAAGCTCGCTGCTACCTCTTCGCGGATAGATTCCGG  >  NC_000913/1855156‑1855641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑