Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,807	G→A	A95V (GCC→GTC)	glpF ←	glycerol facilitator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,807	0	G	A	100.0%	74.5 / NA	23	A95V (GCC→GTC)	glpF	glycerol facilitator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

CCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCAACCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATT  >  NC_000913/4117564‑4118050                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ccGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTtg                                                                                                                                                                                                                                               <  1:170839/251‑1 (MQ=255)  cgcttGGTACACCGTTGCCATCGGCCGTTAACGCCAGGATCCGCCCCATCAGAATAGCGGTAATCTCCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAATTTCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAT‑GCCCGTAACCTAAAGCCGCAGCACAGAAAGCGCCGACAACTtgt                                                                                                                                                                                                                                              <  2:194057/247‑1 (MQ=255)                                        aTCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAAc                                                                                                                                                                                                         >  1:71515/1‑250 (MQ=255)                                              cccATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGCAAGTAGGGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCaa                                                                                                                                                                                                   >  2:189171/1‑250 (MQ=255)                                                 aTCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAg                                                                                                                                                                                               <  1:140112/251‑1 (MQ=255)                                                               aaTCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCAcaatgcaatg                                                                                                                                                                                  <  1:151144/250‑1 (MQ=255)                                                                                              aaaTTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAAc                                                                                                                                                  >  2:141938/1‑251 (MQ=255)                                                                                                    atatGCGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGGGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTACGATGCGCGCCGGAAACCCCT‑Gc                                                                                                                                           >  2:231683/1‑251 (MQ=255)                                                                                                                            aaaGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCAc                                                                                                                   >  2:177010/1‑251 (MQ=255)                                                                                                                                    agccagATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTc                                                                                                           >  1:147896/1‑251 (MQ=255)                                                                                                                                     gccagATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTcc                                                                                                          >  1:128475/1‑251 (MQ=255)                                                                                                                                                acTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAAT‑GGACTAAAAGCCCGAAAACTAAACCCGCAGCACAGAAAGCGCCGAAAACTTTGGAAACGTTAAAAGGAATAACTTGCCGCTGTTGGAAACAGCCAAACGGCAACAATGCAATGGTAAAAGCGGAATTAAGATGC‑CGCCGGAAACCCCTTCCGTTCAGGCAGATGGCCATTGCCACCCCCAGTCCCCaatgaaccc                                                                                               >  2:80421/1‑244 (MQ=255)                                                                                                                                                             ccGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTg                                                                                  <  2:60421/251‑1 (MQ=255)                                                                                                                                                                      aTGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGa                                                                         <  1:221064/251‑1 (MQ=255)                                                                                                                                                                       tgtgATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGAc                                                                        >  2:6496/1‑251 (MQ=255)                                                                                                                                                                        gtgATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAATCCCCAAATGACACTGATTTCCCACTGACCAAAAGACg                                                                       <  1:317513/251‑1 (MQ=255)                                                                                                                                                                                                aaataaatTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTgca                                                >  2:142133/1‑250 (MQ=255)                                                                                                                                                                                                    aaatTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTgcaacgc                                            >  1:125178/1‑250 (MQ=255)                                                                                                                                                                                                              aaa‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCAACCCACAcc                                  <  1:71156/250‑1 (MQ=255)                                                                                                                                                                                                                    ccGTAAACTAAAGCCGCAGCACAGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATACCTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCAACCCACACCgaagaa                            <  2:291713/251‑1 (MQ=255)                                                                                                                                                                                                                                       cacaGAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCAACCCACACCGAAGAAAATCAACAACCCGGTACCg         >  2:21803/1‑251 (MQ=255)                                                                                                                                                                                                                                           gAAAGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCAACCCACACCGAAGAAAATCAACAACCCGGTACCGAGGa     <  1:6496/251‑1 (MQ=255)                                                                                                                                                                                                                                              aGCGCCGACAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCAACCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAAtt  >  1:125841/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     CCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAATAGCGGTAATCACCATCTCAACTGCGAAAGCCTGCACAAAATTGATATGAGGATTAGGGTAAGTAGAGAAAGTGCCAGCCAGATCAACACTTTCAACGCTGCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAA‑GCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCT‑GCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAGACGCACCAGCGACTTTTAGTGCTGCAACGCAACCCACACCGAAGAAAATCAACAACCCGGTACCGAGGAATT  >  NC_000913/4117564‑4118050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑