Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,259	Δ1 bp	coding (718/900 nt)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,259	0	G	.	100.0%	140.4 / NA	31	coding (718/900 nt)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (24/7);  total (24/7)

CATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGC  >  NC_000913/2111017‑2111490                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        catttcatCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGc                                                                                                                                                                                                                                >  1:75015/1‑251 (MQ=255)    ttcatCATTCCTTTTAATTCATCTTGCTCCACCATCACGACCAAGCTGCAAAAACTATTCAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCttt                                                                                                                                                                                                                             >  1:125823/1‑251 (MQ=255)       atcatTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTgcg                                                                                                                                                                                                                          >  1:263490/1‑251 (MQ=255)                tttAATTCATCTTGCTCCACCGTCACGAACAAGAGGCAAAAACTATTACATTGCTGTAGTCGCACATACTTCATTGAGCATTAGTGTCACGCCAACCAGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTCTTAAGGCGAGAGTTATGTGGAAGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTc                                                                                                                                                                                                                 >  2:173853/1‑251 (MQ=255)                          cTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAgc                                                                                                                                                                                                       >  1:45153/1‑251 (MQ=255)                             gCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAgcgc                                                                                                                                                                                                     >  1:369198/1‑250 (MQ=255)                                ccaccaTCAAGAACAAGCGGCAAAAACTATTCAATTGCTGTAGTCGTAAATAATTCATTGAGAATTCGTTTCACGCCAACCTGCAAGTCAGGCAAGACAAGAGCACAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGATATGTGGAGGACGAGCTGTTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTTAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACAAGCgcatc                                                                                                                                                                                                  >  2:239971/1‑248 (MQ=255)                                                     aaaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCATTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTGTTCAAAAACAAGCGCAGCATAATCGTACCAGGTTGTGGtc                                                                                                                                                                            >  2:120734/1‑250 (MQ=255)                                                           tATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTg                                                                                                                                                                      >  1:96249/1‑251 (MQ=255)                                                               aaaTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCt                                                                                                                                                                   >  2:164290/1‑250 (MQ=255)                                                                      tGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATg                                                                                                                                                           >  2:94986/1‑251 (MQ=255)                                                                         aGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGt                                                                                                                                                         <  2:216001/250‑1 (MQ=255)                                                                               aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGcc                                                                                                                                                  >  1:63450/1‑251 (MQ=255)                                                                                   aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAACAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACCAGCCTGCg                                                                                                                                              >  2:133351/1‑251 (MQ=255)                                                                                                    tttCAAGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGTTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTc                                                                                                                               >  1:314835/1‑249 (MQ=255)                                                                                                                        cAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTTGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCAtgtg                                                                                                         <  2:115427/251‑1 (MQ=255)                                                                                                                                       cAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATcagcc                                                                                           >  1:70445/1‑250 (MQ=255)                                                                                                                                                            cTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGgcg                                                                     >  1:272090/1‑251 (MQ=255)                                                                                                                                                                aTTAAGGGAAGAGTTATGTGGACGCCGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTGGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTTCCAGGTTGTGGTCCCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAAGCAGCCAGCAGTTCAGCACCTGTGGGCGCAc                                                                  <  2:328546/250‑1 (MQ=255)                                                                                                                                                                      gCGAGAGTTATGTGGACGCCGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGATGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGAGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATACGGTTTATTCAGTGAGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTAAGCACCTGTTGGCGCACCAAACTg                                                           >  1:142333/1‑251 (MQ=255)                                                                                                                                                                                    gacgacGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCg                                             >  1:175279/1‑251 (MQ=255)                                                                                                                                                                                       gacgaGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCt                                           >  1:93092/1‑250 (MQ=255)                                                                                                                                                                                          gaGCTGGTGTAAGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGTG‑AATGCCTGCTTTGCGCTCCTCTTCAAAAACCAGCGCAGCATAATCGTCCCAGGTTGTGGTACCACTGGCTACCAAATTGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACGGATCGTTAATAACCGCTAAtt                                       <  1:9984/251‑1 (MQ=255)                                                                                                                                                                                                     ggATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCGGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGctct                            >  1:1375/1‑251 (MQ=255)                                                                                                                                                                                                     ggATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGctct                            >  1:63098/1‑251 (MQ=255)                                                                                                                                                                                                       aTAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCttt                          <  1:232023/251‑1 (MQ=255)                                                                                                                                                                                                         aGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTg                        >  1:88052/1‑251 (MQ=255)                                                                                                                                                                                                                    ggTACGGCGTTGAGCGTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAAc             <  2:75015/251‑1 (MQ=255)                                                                                                                                                                                                                         tGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGt        >  1:230136/1‑251 (MQ=255)                                                                                                                                                                                                                          gCGTTGGGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGtt       <  2:96249/251‑1 (MQ=255)                                                                                                                                                                                                                               gAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGAACAAGCATGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGAACCTGTTGGAGCACCAAACTGATCGGTGATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTgac  >  2:283807/1‑249 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        CATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGC  >  NC_000913/2111017‑2111490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑