Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	148.0 / NA	41	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/16);  total (25/16)

TACAGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAGC  >  NC_000913/3329497‑3329989                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          tACAGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCCATTCTGAAATATg                                                                                                                                                                                                                                                        >  2:265776/1‑250 (MQ=255)      tGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCCTCTTATCACCCCGTTACGATGTTCAGCCAGGTACGCACCATCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTa                                                                                                                                                                                                                                                   >  1:34777/1‑250 (MQ=255)            aaaGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGCTGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAgg                                                                                                                                                                                                                                            >  2:202962/1‑251 (MQ=255)             aaGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCACGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGc                                                                                                                                                                                                                                           >  1:211944/1‑251 (MQ=255)                  tGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCgggg                                                                                                                                                                                                                                       >  2:46775/1‑249 (MQ=255)                              ttttATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACC‑TGGaacg                                                                                                                                                                                                                         >  1:325847/1‑248 (MQ=255)                                                 tattaCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAg                                                                                                                                                                                                        <  1:75002/249‑1 (MQ=255)                                                 tattaCCCCGCTACGATGTTCAGCCAGGTACGCACCATCCCCCGTGGTTCTGCCGAAGCGCACTCCTGCGAACTGGCTGTGGTGCCCGGCGACCTGAACCACTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGAATCACC‑CGGGGCGGGACGctgctgaccccaa                                                                                                                                                                                                       >  2:224062/1‑243 (MQ=255)                                                     aCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGGGCGGGAAACTGCGTCGCCCGACT‑c                                                                                                                                                                                                   >  2:266479/1‑249 (MQ=255)                                                            tACGAGGTTCTGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAAc                                                                                                                                                                                           <  2:293561/250‑1 (MQ=255)                                                                 tGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGGTAACGaaact                                                                                                                                                                                     >  1:37147/1‑248 (MQ=255)                                                                          aGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGGACACTGCTGCGCCAGACT‑CAGGGTAACGAACCTGGAACGGt                                                                                                                                                                             >  1:170783/1‑250 (MQ=255)                                                                               cGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAAAACTGCTGCGCCCGACT‑CAGGGTAACGAACCTGGAACGGTAGTT‑Gc                                                                                                                                                                      >  2:161527/1‑251 (MQ=255)                                                                                gCACCCTCACCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGGTAACGAACCTGGAACGGGAGTT‑Gcc                                                                                                                                                                     >  1:91765/1‑251 (MQ=255)                                                                                            gTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGTAATATGAGTTAAAACAGGCGGGGATCACC‑TGGGGCGGGACACTGCTTCGCCCGACT‑CTGGGTAAAGAACCTGGGACgggcggttccaagatacagtcg                                                                                                                                                          >  2:345741/1‑231 (MQ=255)                                                                                            gTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGGTAACGAACCCGGAACGGTAGTT‑GCCAGGAAACAGTCg                                                                                                                                                         >  1:117044/1‑251 (MQ=255)                                                                                                     ccGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCCTGG‑GCGGAACACTGCTTCGCCACACT‑CAGGGTAACGAACCTGGGACGGTAGTT‑GCCCGGAAACAAGCGGCCCCGccg                                                                                                                                                >  2:118076/1‑249 (MQ=255)                                                                                                     ccGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTg                                                                                                                                                <  1:161527/251‑1 (MQ=255)                                                                                                      cGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGACATATGAGTTAAAACAGGCGGGGAACACC‑TGGAGGGGAACACTGCTGCGCCAGACG‑CAGGTGAACGAACCCGGAACGGCAGGT‑Gcccgacaacaaccggacccgcggc                                                                                                                                               >  2:261095/1‑229 (MQ=255)                                                                                                         aGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACg                                                                                                                                            <  1:175718/251‑1 (MQ=255)                                                                                                           cgcAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGAt                                                                                                                                          <  1:233232/251‑1 (MQ=255)                                                                                                               aTACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGc                                                                                                                                      <  1:270109/251‑1 (MQ=255)                                                                                                                     cGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑CGGAGCGGAACACTGCTGCGCCCGACT‑CAGGTTAACGAACCTGGAACGGGAGTT‑GCCCGTAAACCGTCGGCCCCGCCGCACGCTCCGCTTatg                                                                                                                                 >  2:282959/1‑248 (MQ=255)                                                                                                                       aaCTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCCGCCACAACGTTCTGAGCCGCTCCCGTTGGCCTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCCATTCTGAAATATGAGTTAAAACCGGCGGGTATCACC‑CGGAGGGGAACCCTGCTGCGCCCCAAT‑AAGGCTTACGAAACTGGAACGGTCATT‑GACAGGAAACAGTCGGCCCCGCTGCaagaactacctacgctt                                                                                                                              >  2:148718/1‑235 (MQ=255)                                                                                                                           ggATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATg                                                                                                                           <  1:105032/250‑1 (MQ=255)                                                                                                                                  gtgaaaggagaaatGAACCGCTTTACGCTGACGCGATGCATGCCCCAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGCGCCCTTCTGACCTATGCGTTAAAACAGCCGGGTATCCCC‑TGGCGCGCACCACCTCTGCCCCCAACT‑CACGCTAACGAACCTGGACCGGCCATT‑TCCCGTAAACCGTCGGCCCCGCTTCCCGATCTTCTTCAGATTTTGCTTaaac                                                                                                                    >  1:219292/14‑249 (MQ=255)                                                                                                                                         gCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGac                                                                                                            <  2:20866/251‑1 (MQ=255)                                                                                                                                               tGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGGACGGGAGGT‑GCCCGTAAACAGACGGCCCCGCTGGACGATCTGCTTTAGATTATGCTTAAAAAGTCGGgcaaaaa                                                                                                       >  2:242863/1‑247 (MQ=255)                                                                                                                                                       tttACGCAGACGGGTTGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCAAGCTATGCCGGTGCACTTCTGCAATATGAGTTAAACCAGGCGGGTATAACC‑TGGCGCGGCAAACAGCTGCGGCAAACAACAGGT‑AACGAAACTGGCACGGTAGTTTGCCA‑TAAAAACTCGGCCTCCCTTCACCATATGCCTAAGATTATTCTTAAAAAggtcgacacactgaacagagc                                                                                              >  2:338089/1‑231 (MQ=255)                                                                                                                                                               gACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGt                                                                                        <  2:317212/249‑1 (MQ=255)                                                                                                                                                                       gcctgccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCAt                                                                               <  1:269148/250‑1 (MQ=255)                                                                                                                                                                          tgccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAAACTGGAACGGGAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGAACTGCTTTAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGGTTTCCGCATGAt                                                                            >  1:149433/1‑250 (MQ=255)                                                                                                                                                                           gccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGAt                                                                            <  2:190246/249‑1 (MQ=255)                                                                                                                                                                           gccACAACGTTCGGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTGTCACC‑TGGAGCGGAACACTGCTGCGCCATACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCTTTAAACAGTCGGTCTCTCTGCATGATCTTCTTAAGATTAGTCTGAAAGAGTCGGTCAACATGATCGCCGATACGCTTTGCCGCATGATa                                                                           <  2:169974/250‑1 (MQ=255)                                                                                                                                                                                                cccGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGc                                                     <  1:278223/251‑1 (MQ=255)                                                                                                                                                                                                    ttGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGAAT‑CAGGGTAACGAACCTGGAACGGGAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGGTCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTTCGCCTGATAGGCCCTGCGCGCTTCAATGTGCCTgg                                                 >  1:228855/1‑251 (MQ=255)                                                                                                                                                                                                                  gCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCg                                   <  2:74254/251‑1 (MQ=255)                                                                                                                                                                                                                                         gTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACAATGCTGCGCCAGACT‑CAGGTTAACGAAACTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGGCGGAACACATGATCGCCGATACGGGTTTCCGCATTAAAGGCCATGCGCGCTTCCATGTGCCTGGAACATggcggggcggggcgggagccctgggtccaaac            >  1:202898/1‑234 (MQ=255)                                                                                                                                                                                                                                           gCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATcc           <  1:118076/250‑1 (MQ=255)                                                                                                                                                                                                                                            cAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGGCGGACGCCGTGCGGCAGATCCtt         >  1:68289/1‑250 (MQ=255)                                                                                                                                                                                                                                                   gAAATATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAGc  <  1:170487/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          TACAGTGCCCCAAAGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACC‑TGGAGCGGAACACTGCTGCGCCAGACT‑CAGGTTAACGAACCTGGAACGGTAGTT‑GCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAGC  >  NC_000913/3329497‑3329989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑