Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,287,464	T→A	intergenic (‑93/‑147)	acs ← / → ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,464	0	T	A	100.0%	117.7 / NA	32	intergenic (‑93/‑147)	acs/ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/14);  total (18/14)

GGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTG  >  NC_000913/4287220‑4287710                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gggttGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGc                                                                                                                                                                                                                                                   >  1:204616/4‑250 (MQ=255)              cAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGCGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAACGTAGAATtgt                                                                                                                                                                                                                                     >  2:183354/1‑251 (MQ=255)                     ttttCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAAt                                                                                                                                                                                                                               <  1:183354/250‑1 (MQ=255)                                      aGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGGAGGATGTTAATAATATGTGGCATAAGCGTGAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtttt                                                                                                                                                                                                             <  1:30491/251‑1 (MQ=255)                                       gAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAttttt                                                                                                                                                                                                            <  1:80823/251‑1 (MQ=255)                                            tATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAgg                                                                                                                                                                                                       >  1:70570/1‑251 (MQ=255)                                                                   tgATACATCGCCTCGTACTGCTGAGGGTTTATCCGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCCGGGTAAttt                                                                                                                                                                                                              >  2:212100/1‑221 (MQ=255)                                                                   tgATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTGAGTAGTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAttt                                                                                                                                                                                                              <  1:212100/221‑1 (MQ=255)                                                                       aCATCGCCTCGTACTGCTGAGGGTTTATCAGGCAAAGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCAGGCTTTTGTGCTCCTTGTAGGAGGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAAGTGTGCAAATAAACGGCAGGGTAATTTGTGAAGGTCTGCAACAAAAGTTGACTAATTCtt                                                                                                                                                                             <  2:39726/250‑1 (MQ=255)                                                                         aTCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTg                                                                                                                                                                           >  2:64153/1‑250 (MQ=255)                                                                                cGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACAt                                                                                                                                                                   >  2:65435/1‑251 (MQ=255)                                                                                            ggTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGTGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatgcat                                                                                                                                                       <  1:45613/251‑1 (MQ=255)                                                                                                   cAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGGAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTGTGAGGAACATGCAGTGATGCATGCTGtt                                                                                                                                                 <  2:231424/250‑1 (MQ=255)                                                                                                     ggCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGaa                                                                                                                                              <  2:186543/251‑1 (MQ=255)                                                                                                                           aGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAAc                                                                                                                        >  2:118387/1‑251 (MQ=255)                                                                                                                                           tgAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGCTGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGGAGAATTGTGCAAATAAACGGCAGGGGAATTTTTGAAGGTCAGCAACAAAAGATGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTAtttt                                                                                                        >  2:9669/1‑251 (MQ=255)                                                                                                                                                             ttGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTg                                                                                       <  1:285614/250‑1 (MQ=255)                                                                                                                                                               gTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGTAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGAtt                                                                                    <  1:228177/251‑1 (MQ=255)                                                                                                                                                                    ccTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTaa                                                                                  >  1:264394/1‑248 (MQ=255)                                                                                                                                                                        gTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGacaac                                                                           >  1:125932/1‑251 (MQ=255)                                                                                                                                                                          aGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGGAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATTCACTTACAATTGATTAAAGACAACAt                                                                         >  2:83489/1‑251 (MQ=255)                                                                                                                                                                                                  aGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTacac                                                 >  1:198699/1‑251 (MQ=255)                                                                                                                                                                                                     gTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATa                                              <  1:325590/251‑1 (MQ=255)                                                                                                                                                                                                        aaaTGAAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAggg                                           >  1:308462/1‑251 (MQ=255)                                                                                                                                                                                                                gggTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTTATTTTTGAATGTCAGCAACAAAAGTTGATTAATTCTTTGATGAACATGCAGTTATGCTTGCTGTTGAAAAAGAGGAATATACTGACTAACTCTAAAGTGGTATTTTACATGCCCTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGAGCGAGCaa                                   <  1:277102/251‑1 (MQ=255)                                                                                                                                                                                                                       ggCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATg                            >  2:1057/1‑251 (MQ=255)                                                                                                                                                                                                                         cAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGAc                          >  1:309357/1‑251 (MQ=255)                                                                                                                                                                                                                         cAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGAc                          <  2:314869/251‑1 (MQ=255)                                                                                                                                                                                                                                 tttAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAgg                  >  1:369960/1‑251 (MQ=255)                                                                                                                                                                                                                                           gggCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACt        <  1:139510/251‑1 (MQ=255)                                                                                                                                                                                                                                                aCAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGt   >  2:72600/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTg  >  1:182464/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTG  >  NC_000913/4287220‑4287710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑