Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,539,947	C→G	A882P (GCG→CCG)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,539,947	0	C	G	100.0%	122.8 / NA	33	A882P (GCG→CCG)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/6);  total (27/6)

GGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCGGATGAATAAACGGATGCATAT  >  NC_000913/1539747‑1540189                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   ggCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACtt                                                                                                                                                                                                  >  2:369129/1‑251 (MQ=255)          aaTCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCCGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCt                                                                                                                                                                                         >  2:138715/1‑251 (MQ=255)                         cTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCg                                                                                                                                                                          >  1:338815/1‑251 (MQ=255)                             gCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCgg                                                                                                                                                                         >  2:29472/1‑248 (MQ=255)                                  gCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATAGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGGTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGGCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGCGTCa                                                                                                                                                                  >  2:261067/1‑250 (MQ=255)                                    cATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGc                                                                                                                                                               >  1:179591/1‑251 (MQ=255)                                       cACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGt                                                                                                                                                            >  1:193534/1‑251 (MQ=255)                                       cACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGt                                                                                                                                                            >  2:271372/1‑251 (MQ=255)                                            ttGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAgggg                                                                                                                                                       >  1:192534/1‑251 (MQ=255)                                            ttGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAgggg                                                                                                                                                       >  1:272267/1‑251 (MQ=255)                                                tgtAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGgtgt                                                                                                                                                   >  2:273855/1‑251 (MQ=255)                                                     ttGAGTTTGCCGAGGAAATAGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCTAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACAAGGCTGTGAGAGCTCTGGCGGCGAGTAAAGCAGTAGGGGATGTCATAc                                                                                                                                              >  2:34353/1‑251 (MQ=255)                                                     ttGAGTTTGAAGAGGAACTCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCACGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGCCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGCGAGTCATGCAGTAGGGGTTGTAATAc                                                                                                                                              >  2:162300/1‑251 (MQ=255)                                                          tttGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCCGTAGGGGTTGTAATACCACGt                                                                                                                                         >  1:168885/1‑251 (MQ=255)                                                               cGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGtt                                                                                                                                    >  2:5791/1‑251 (MQ=255)                                                               cGAGGAAATCGATTTCATCCTGGGACTTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCAATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGGATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCCCCCGGCTGTGAGAGCTCTGCCGGAGTGTCCTGCACGAGGGGTTGTAATAACCCGTCGGtt                                                                                                                                    >  2:305195/1‑251 (MQ=255)                                                                     aaTCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTg                                                                                                                              >  1:130908/1‑251 (MQ=255)                                                                                         ttCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGGTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGCGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCccac                                                                                                          >  2:358519/1‑251 (MQ=255)                                                                                               gAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCg                                                                                                     >  2:130815/1‑250 (MQ=255)                                                                                                      cTTTACCCCCATTGCCAAGTTTGAACATCAATGGCCCGCCCGAGGTAAAGCGTTCATACGTACCAGGGC‑AGTCGCGCTCCCCCGCCCCAATATTCGGCCGGGATTTGCCTGGAATCAGATCGCATTCCCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTCCCGGAGAGCCATGCAGTAGGGGTTGTAATCCCACGCCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAACGCt                                                                                             >  2:33959/1‑251 (MQ=255)                                                                                                                aTTGCCAAGTTTGTCCATCAATGGCCAGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGAtcc                                                                                   >  1:297173/1‑249 (MQ=255)                                                                                                                      aaGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGt                                                                             >  2:267862/1‑251 (MQ=255)                                                                                                                               cATCAATGGCCCGAGCGAGGTAAAGCGTTCTTACGTAG‑AGGGTTATCCGCGTCCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGTCGATACCTTTGTAGATTTccc                                                                    <  1:206420/251‑1 (MQ=255)                                                                                                                                ataaggggCCCGGGGGAGGAAAAGCGTTCAGGCGGAGGAGGGG‑AGCCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCa                                                                   <  2:192534/245‑1 (MQ=255)                                                                                                                                      ggCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCt                                                             >  2:244458/1‑251 (MQ=255)                                                                                                                                        cccGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGc                                                           >  1:59576/1‑251 (MQ=255)                                                                                                                                              cgagGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGAtt                                                     >  1:160004/1‑251 (MQ=255)                                                                                                                                                                  tAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGAccgcc                                 >  1:308226/1‑251 (MQ=255)                                                                                                                                                                             gtccccccccACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCg                       <  1:281382/243‑1 (MQ=255)                                                                                                                                                                             gccccgccccACCGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCg                       <  1:369129/243‑1 (MQ=255)                                                                                                                                                                                  ccccccccGCCACAATATTCGGCGGGGTTTTGCCTGGAATCAGATCGCATCCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGGGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTACCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCGGATGa                  <  2:147088/245‑1 (MQ=255)                                                                                                                                                                                            cacaATATTCGGCGGGGTTTTGCCTGGAATCCGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCGGATGAATAAACGGAt        >  1:78415/1‑251 (MQ=255)                                                                                                                                                                                                  atTCGGCGGGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGTAGAGTCATGCAGTAGGTGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATTCTTTTGTAGATTTCCCAGTCGCCGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCGGATGAATAAACGGATGCatat  <  1:363541/251‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   GGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCATCCTGGGTATTCCACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGT‑AGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCGGATGAATAAACGGATGCATAT  >  NC_000913/1539747‑1540189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑