Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	97.0%	92.2 / ‑6.6	33	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (13/19);  minor base A (0/1);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

CCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCAT  >  NC_000913/1358622‑1359103                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      ccAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTTGCCAACAAGACGTTAATCATCGGCACAAAGAGACAATCCAGCAGCgc                                                                                                                                                                                                                                          >  2:149503/1‑250 (MQ=255)    aCGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATg                                                                                                                                                                                                                                        >  2:70740/1‑250 (MQ=255)                      aGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGGACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATGCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATGATCGGCAAAAAGAGACATTCCAGCAGCGATTGCCAGCCGAcctgatatcc                                                                                                                                                                                                                     <  2:38426/251‑9 (MQ=255)                        gcccTGAACTTCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGa                                                                                                                                                                                                                   <  1:322587/250‑1 (MQ=255)                                           aTAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGCTTCGCGGCGGTTAACACGGCCACGAAGGTTAAAAACCAAACCCACGGCAGCACTTCCGGGGAGAGGGCGGAGAGATAGAATTTAGCCAACAAAACGGTAATCATCGGCAAAACGAGACAATCCCGCAACGACGACCAGCCGACCATCAATCCGACGAGAGGGGTAATCGACtt                                                                                                                                                                                                >  1:168800/1‑251 (MQ=255)                                           aTAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAAGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGCGGGCGGGGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCCAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAAACCGCAGGGCGGGTTAATCGACtt                                                                                                                                                                                                >  2:97386/1‑251 (MQ=255)                                                    accaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAAACCGACGGGCGGGTTAATCGACTTTTGcgcgg                                                                                                                                                                                        >  1:78546/1‑249 (MQ=255)                                                            ggAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCATATTCGCGGCGGTTAAAATGGCGACGAAGGTTTCCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTTCGGGTTAATCGACTTTGGCGCGTAGGTATAg                                                                                                                                                                                <  2:49386/250‑1 (MQ=255)                                                              agaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGcc                                                                                                                                                                             <  2:310688/251‑1 (MQ=255)                                                               gaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCg                                                                                                                                                                            <  1:243651/251‑1 (MQ=255)                                                                      cccagtccacaccgggtatgcaaGTTAGCGACCCGGTTGACGCTCTTCCGATGCGAGGCGGTTAAAATGGCGACGAAGGTGACCACCCACACCCACGGCGTCACTTCCGGGAAGATGGCGGAGATATAGAGTATCGCCAACATCACTTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAACCCGACGTGCGGGTTAATCGACTTTTTCGCGTAGGTATAGGCCGAAcctgc                                                                                                                                                                     <  2:74638/230‑4 (MQ=255)                                                                           tACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTGCCACCCCCACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAAATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCCGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGGAGGTATAGGCCGAACC‑GGCCTccc                                                                                                                                                               >  2:183301/1‑250 (MQ=255)                                                                                    ggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGc                                                                                                                                                      <  1:307435/251‑1 (MQ=255)                                                                                         tGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAAc                                                                                                                                                  <  1:55304/250‑1 (MQ=255)                                                                                                  cGACCAGGTTGACGCGCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCg                                                                                                                                        <  1:195909/251‑1 (MQ=255)                                                                                                        ggTTGACGCTCTGCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTg                                                                                                                                  <  2:302070/251‑1 (MQ=255)                                                                                                                           cgcgGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACCTCCGGGACGAGGGCGGAGAGATAGCTTTTCGCCCACAAGCCCTTAATCATTGGCAAACAGAGACAATACAGCAGCGATGACCAGCCGGCCCTAAAACCGACGTGCCGGTTAATCGACTTTTGCGCGGAGGCATAAGGCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGAAAGCGGTAAAAAGCccac                                                                                                               >  2:66078/1‑251 (MQ=255)                                                                                                                                      aaaTGGCGACGAAGTTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGCCCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCcgccagcgcc                                                                                                    <  1:12766/251‑1 (MQ=255)                                                                                                                                                 aaggtacccacccacACCCACGGCGGCCCTGCGGGGAAGAGGGCGGAGAGATAGATTTCCGCAAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGCCCAGCCGACCATAAATCCGACGGGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCCCCGGAAACGGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGa                                                                                         <  1:136749/244‑1 (MQ=255)                                                                                                                                                         cacccacacccaCGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGAAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGGGGGTTAAGCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTGGCGGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGaa                                                                                 <  1:129448/251‑1 (MQ=255)                                                                                                                                                                                   ggAAGAGGGCGGAGAGATAGATTTTCGCAAACAAGACGTTAATCATCGGAAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACGGGCGAACCAGTTTGCCGTAGCTGAGAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACa                                                       <  1:156344/251‑1 (MQ=255)                                                                                                                                                                                    gAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGca                                                                                                                  >  1:96625/1‑191 (MQ=255)                                                                                                                                                                                    gAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGca                                                                                                                  <  2:96625/191‑1 (MQ=255)                                                                                                                                                                                     aaGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAAt                                                     >  1:310967/1‑251 (MQ=255)                                                                                                                                                                                            gggAGAGATAGATTTTCGCCAACAAGACGTTATTCATCGGAAAAAAGAGACAACCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGGGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCCCCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCaaaa                                              <  1:289085/250‑1 (MQ=255)                                                                                                                                                                                              gagagaTAGTTTTTCCCCAAAAAGACGTTAATCACCGGAAAAAAGAGACAATCCAGCAGCATTGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGTCTTTTGCGCGTAGGTAGAGGCCGAACC‑GGCCACCGGAACCGGGCGAACCAGTTTGCCGTAGCTGATGGCGGTAAACAGCCCCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGt                                            <  2:246664/251‑1 (MQ=255)                                                                                                                                                                                                    aagatTTTCGCAAAAAAGACGTAAATCATCGGCAAAAAGAGAAAATCCAGCAGCGATGACCAGCCGACAATAAATCCGACGTGCGGGTTAATCGACTTTGGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACGGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCaaa                                      <  1:70740/250‑1 (MQ=255)                                                                                                                                                                                                            cgcaaacaaGACGTTATTCATCGGAAAAAAGAAACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGTTTAATCGACTTTTGCGGGTAGGTATAGGC‑GAACCCGGCCTCGGGAACCGGGCGAACCAGTTGGCCGTAGTTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCa                              <  1:155520/247‑1 (MQ=255)                                                                                                                                                                                                                      aCGTTAATCATCGGCAAAAAGAGACAACCCAGCAGCGATGCCCAGCCGACCATAAATCCGGCGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGCCCGAACC‑GGCCTCGGAAAACTGGCGACCCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTgag                    <  1:32172/251‑1 (MQ=255)                                                                                                                                                                                                                       cGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTg                      >  1:266036/1‑248 (MQ=255)                                                                                                                                                                                                                       cGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTg                      >  1:228228/1‑248 (MQ=255)                                                                                                                                                                                                                          tAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGAt                  >  1:296412/1‑249 (MQ=255)                                                                                                                                                                                                                                        aaGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcatcat  >  1:310166/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      CCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACC‑GGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCAT  >  NC_000913/1358622‑1359103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑