Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,016,006	G→A	I155I (ATC→ATT)	fabA ←	beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,016,006	0	G	A	96.9%	106.3 / NA	32	I155I (ATC→ATT)	fabA	beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (22/9);  minor base C (1/0);  total (23/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCCACATTGCGTCCAGGCCCAGGCATCCCGGCATAACCGGATCGCC  >  NC_000913/1015768‑1016251                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       ggCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGAccc                                                                                                                                                                                                                                            >  1:36635/1‑250 (MQ=255)              ggACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCACAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACtt                                                                                                                                                                                                                               >  1:179402/1‑251 (MQ=255)                 cGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGc                                                                                                                                                                                                                            >  1:310295/1‑251 (MQ=255)                          gAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCCCTTCGCCATCCGCCa                                                                                                                                                                                                                   <  2:285070/251‑1 (MQ=255)                                    ccTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCAGCCGCCAGGCCCATAAt                                                                                                                                                                                                         <  2:345299/251‑1 (MQ=255)                                         cAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGACGCCATGGCGGACAGGGTCGTAATGGCCTGATTCTGTATCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTAGCCTTTTGATACTCTGTCTGATTATAATCAGAAGCCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGATGGCGGTATAAATCAGACGACCATCAACCCGAACTTCGCCATCCGCCAGGCCCATAATCAgac                                                                                                                                                                                                    >  1:209716/1‑251 (MQ=255)                                                 gAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGATGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATTCGCCAGGCCCATCATCAGACGACGGtt                                                                                                                                                                                             >  2:1531/1‑250 (MQ=255)                                                               aaTGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTaa                                                                                                                                                                               >  1:267222/1‑250 (MQ=255)                                                                                 gagaAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCAc                                                                                                                                                            <  1:273429/251‑1 (MQ=255)                                                                                        cATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCAATTCGCCATCCGCCAGGCCCATAATCAGACGACGGTGAACAATGCGTTTAAAGTGAATAGGGTAGGTCACTTTTTTc                                                                                                                                                     <  2:183154/251‑1 (MQ=255)                                                                                                             ggCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAc‑c                                                                                                                                >  1:185378/1‑250 (MQ=255)                                                                                                                         tctcTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGCCCGACTTTCAGGTCGCTGGCGGTATAAATCAGCCGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCCTAATCAGACGCCGGTTAACAATTCGTTTACAGTGAATCCGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGTC‑CAGTGATTTTCACt                                                                                                                   >  2:216644/1‑251 (MQ=255)                                                                                                                                          cTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAgcgc                                                                                                  >  2:279190/1‑251 (MQ=255)                                                                                                                                                                 ttttGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGccc                                                                            >  2:93931/1‑250 (MQ=255)                                                                                                                                                                  tttGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCccac                                                                          >  1:100597/1‑250 (MQ=255)                                                                                                                                                                    tGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTAAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGAGAATACGGTAGGTCACTTTTTTCGCTGTCAGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCccagcc                                                                        >  2:368856/1‑251 (MQ=255)                                                                                                                                                                      aTACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCccagcc                                                                        >  1:179202/1‑249 (MQ=255)                                                                                                                                                                             gtctgATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGTATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAggg                                                               >  2:231689/1‑250 (MQ=255)                                                                                                                                                                                  aTTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAAc                                                          <  1:218548/251‑1 (MQ=255)                                                                                                                                                                                      taATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCt                                                       >  1:287688/1‑250 (MQ=255)                                                                                                                                                                                        aTCAGAAGGCCGACGTATCCTGGAACAGACCGTCTTTCAGATCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATAAGACGACGGATAACAATGCGCTAAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACACA‑TAAATTTAACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCCGCAAGCCGAGGTAAAACCCCAcc                                                    >  2:256942/1‑251 (MQ=255)                                                                                                                                                                                        aTCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGCTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTAcc                                                    >  2:256933/1‑251 (MQ=255)                                                                                                                                                                                            gAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCt                                                <  2:310295/251‑1 (MQ=255)                                                                                                                                                                                                 cAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGCATACATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGCAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCCGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCcac                                           >  1:238313/1‑251 (MQ=255)                                                                                                                                                                                                  aGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCcac                                           >  2:315674/1‑250 (MQ=255)                                                                                                                                                                                                          ccTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCGTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCCACATTGCGTcc                                  <  2:169071/251‑1 (MQ=255)                                                                                                                                                                                                             ggAACAGACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCCACATTGCGTccagg                               >  2:81675/1‑251 (MQ=255)                                                                                                                                                                                                                   gACCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAAGCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGAAGAACCCTACCAGCTGCCACATTGCGTccaggcccagg                         >  2:174605/1‑251 (MQ=255)                                                                                                                                                                                                                    aCCGACTTTCAGGTCGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCCACATTGCGTccaggcccaggc                        <  1:93931/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGCTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCCACATTGCGTCCAGGCCCAGGCATCCCGGCATAAc           <  1:124357/250‑1 (MQ=255)                                                                                                                                                                                                                                    cTGGCGGTATAAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCCACATTGCGTCCAGGCCCAGGCATCCCGGCCTAACCgg        >  2:307347/1‑251 (MQ=255)                                                                                                                                                                                                                                           tataAATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCACCCAGCCGAGGTAGAACCCTACCAGCTGCCACATTGCGTCCAGGCCCAGGCATCCCGGCATAACCGGATCGcc  >  2:2517/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       GGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGAC‑CAGTGAATTTCACTTCGCCAACGCCCAGCGCGCGGCCTTTACCTTCGCCGCCCAGCCAGCCGAGGTAGAACCCTACCAGCTGCCACATTGCGTCCAGGCCCAGGCATCCCGGCATAACCGGATCGCC  >  NC_000913/1015768‑1016251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑