Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,061,021	G→A	S192N (AGC→AAC)	yihL →	putative DNA‑binding transcriptional regulator YihL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,061,021	0	G	A	100.0%	89.6 / NA	25	S192N (AGC→AAC)	yihL	putative DNA‑binding transcriptional regulator YihL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/12);  total (13/12)

CGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAGCAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGA  >  NC_000913/4060785‑4061266                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      cgcgCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGc                                                                                                                                                                                                                                          <  1:295336/250‑1 (MQ=255)    gcCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGtt                                                                                                                                                                                                                                      >  1:231909/1‑251 (MQ=255)        cTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGc                                                                                                                                                                                                                                  >  2:244534/1‑251 (MQ=255)                 gTGACGCCATCAACTGAACGCTATCGCAGCACCGGATGGGGGGAGCTGGAAGGACATAAATTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACGTGAAAACCACTCATTTTCTGCAGTCTGGTAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGca                                                                                                                                                                                                                         <  2:102997/251‑2 (MQ=255)                                              cACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACAGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGcc                                                                                                                                                                                             <  2:292239/250‑1 (MQ=255)                                                                               tttCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTg                                                                                                                                                           >  1:19610/1‑251 (MQ=255)                                                                                    aTCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGaa                                                                                                                                                         <  2:278900/248‑1 (MQ=255)                                                                                                    tAATCCTGAAGTTGCTCCGGGCTTTATTGCACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGGATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGGCCAAATAGATATTGAATATTGGCGATTTGAGGCCg                                                                                                                                      >  2:176742/1‑251 (MQ=255)                                                                                                          tGAAGTTGCTAGGGGCTTTATTGAACACCTTGCAAACCACTCATTTTATGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTACAAAAATTGATTTTCACACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGGCCCGACCAGCAAGGCAATATTGTCCAAATAGATATTAAATATTGGCGATTTGAGGCCGTAGAcc                                                                                                                                >  2:224840/1‑251 (MQ=255)                                                                                                                 gCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTcatcat                                                                                                                         <  1:269284/251‑1 (MQ=255)                                                                                                                        gCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTGCCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCATGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATAGTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGGCCTCATCATTAATCTg                                                                                                                  <  2:254712/251‑1 (MQ=255)                                                                                                                            tATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAg                                                                                                               <  2:331325/250‑1 (MQ=255)                                                                                                                             aTTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAgg                                                                                                              <  2:85920/250‑1 (MQ=255)                                                                                                                                   caacTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTTAGGCCGTAGACCTCATCATTAATCTGTAGGGGttttt                                                                                                       >  2:286023/1‑250 (MQ=255)                                                                                                                                              ccACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCCGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACaat                                                                                             >  2:188854/1‑250 (MQ=255)                                                                                                                                                            cAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCGCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                              <  2:153174/251‑1 (MQ=255)                                                                                                                                                             aGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGGGTTTTATGGTGACAATAAATAACGCaagaa                                                                               >  1:301702/1‑249 (MQ=255)                                                                                                                                                                  gggAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAACCCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGAACAGCCAGGCAATATTGTCCAAATCGCTATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGAAAATAAATAACGCAAGAAAGATTCt                                                                        >  1:160032/1‑251 (MQ=255)                                                                                                                                                                   ggAAAAGAGCTACCACAAAGAGACGGAAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAATTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGTAATATTGTCCAAATAGATGTTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGGGTTTTATGGTGACCATAAATAACGCAAGAAAGAttat                                                                        >  1:275688/1‑248 (MQ=255)                                                                                                                                                                           gCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGGGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACgtgt                                                               >  1:276989/1‑251 (MQ=255)                                                                                                                                                                                  aaaaGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACAct                                                        >  1:232694/1‑251 (MQ=255)                                                                                                                                                                                                      aaTTGATTTTCAAACGCGTCAGAATGCCGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGGAGGCCAGCGATATTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGGCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGAACTCATCATTAATCTGGAGGTGTTTTATGGTGACAATAAATAAAGCAAGAAAGATTCTACAAAGGGGCGACACTCTTTATCTTTATTTAAATgg                                    >  2:61992/1‑250 (MQ=255)                                                                                                                                                                                                                                 cGGGCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt         <  1:139837/251‑1 (MQ=255)                                                                                                                                                                                                                                  gggCGATATCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTg        <  2:232694/251‑1 (MQ=255)                                                                                                                                                                                                                                         atCAACAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCGTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAaga  <  1:141873/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      CGCGCCACTGATTGCAGTGACGCCATCAACTGAACTCTATCGCATCACCGGCTGGGGGGCGCTGGAAGGACATAAAGTTTTCTATCACGAAACATATATTAATCCTGAAGTTGCTCCGGGCTTTATTGAACAACTTGAAAACCACTCATTTTCTGCAGTCTGGGAAAAGTGCTACCAAAAAGAGACGGTAGTAAAAAAATTGATTTTCAAACCCGTCAGAATGCCGGGCGATATCAGCAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGA  >  NC_000913/4060785‑4061266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑