Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,813 Δ2 bp coding (142‑143/1161 nt) metB → O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,8110C.92.3% 161.5 / 6.5 39coding (140/1161 nt)metBO‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base . (21/15);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,128,8120G.94.7% 160.6 / 2.3 39coding (141/1161 nt)metBO‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (21/15);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGT  >  NC_000913/4128561‑4129036
                                                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                
cTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtc                                                                                                                                                                                                                                   >  1:344482/1‑251 (MQ=255)
   aaTCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtc                                                                                                                                                                                                                                >  1:263783/1‑249 (MQ=255)
      cTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtcgcg                                                                                                                                                                                                                             >  2:25664/1‑246 (MQ=255)
       tataCGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGCTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtcgcgg                                                                                                                                                                                                                            >  2:120458/1‑245 (MQ=255)
             cAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAAccc                                                                                                                                                                                                                    >  1:312535/1‑251 (MQ=255)
                        aGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGAtg                                                                                                                                                                                                         >  1:349377/1‑251 (MQ=255)
                          aTGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGAtgtg                                                                                                                                                                                                       >  1:105980/1‑251 (MQ=255)
                               cAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGAGTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCa                                                                                                                                                                                                  <  1:26895/251‑1 (MQ=255)
                                  atgaaTTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGCCAAAGCCCAGGGAACTTCATCACATGACACGTAAACAGGCCACCATCGAAGCGCGTAGCGGGTTAAATGACGAAGAACAGCATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCCTCGC‑‑CAACCAAACGCGCGATGTGGTTCAGCg                                                                                                                                                                                               >  2:234667/5‑251 (MQ=255)
                                  aTGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCg                                                                                                                                                                                               <  2:192885/251‑1 (MQ=255)
                                     tATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTgc                                                                                                                                                                                            >  1:81650/1‑251 (MQ=255)
                                                  tAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACt                                                                                                                                                                                <  2:263783/250‑1 (MQ=255)
                                                   aaCACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTgg                                                                                                                                                                              <  2:17805/251‑1 (MQ=255)
                                                        aaTGTTGACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATTACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTA‑‑CGCGACGCGGCAACCCAACGCGCGAGGTGGTTCAGCGTGCGCTGGCAGATCTGGAAggt                                                                                                                                                                         <  2:171830/251‑1 (MQ=255)
                                                         aTGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTGTCCAGCTCCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCTCGATGGGGTTCAGCGTGCGCTGGCAGAACTGGAAggtg                                                                                                                                                                        <  1:228457/251‑1 (MQ=255)
                                                                  ctGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgctggtg                                                                                                                                                               >  2:137858/1‑251 (MQ=255)
                                                                       gCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGt                                                                                                                                                           >  2:240052/1‑250 (MQ=255)
                                                                            tcATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTCAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtacttac                                                                                                                                                     >  2:262977/2‑251 (MQ=255)
                                                                             cATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtacttact                                                                                                                                                    <  1:271832/251‑1 (MQ=255)
                                                                                ttCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACCGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAAt                                                                                                                                                 >  1:36111/1‑251 (MQ=255)
                                                                                      gacaacccccAGGGAACTTCATCACCAGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGc                                                                                                                                           >  1:285795/8‑251 (MQ=255)
                                                                                            gCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTcc                                                                                                                                     >  1:88753/1‑251 (MQ=255)
                                                                                                aGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCcgcg                                                                                                                                  >  2:134506/1‑250 (MQ=255)
                                                                                                aGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCcgcg                                                                                                                                  >  2:239714/1‑250 (MQ=255)
                                                                                                                   cgcgTAAAACGGACACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGt                                                                                                              >  1:270250/1‑251 (MQ=255)
                                                                                                                                caccaTCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCt                                                                                                 >  1:50151/1‑251 (MQ=255)
                                                                                                                                   caTCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGc                                                                                              >  2:286291/1‑251 (MQ=255)
                                                                                                                                       gCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATc                                                                                          <  2:285795/251‑1 (MQ=255)
                                                                                                                                                    ggTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGcgcc                                                                             <  2:80492/251‑1 (MQ=255)
                                                                                                                                                     gTTAAATGTCGACGTACAGTATGGGTGCGTTGTCCCACCGATCCATCTTTCCAGCCCCTATATCTTTACCGGATTTAATGAACCGCGCGCGCATGATT‑‑TCTCTTCGCGGCACCCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCTCCTGGTAACGATCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGcgccg                                                                            <  2:13395/251‑1 (MQ=255)
                                                                                                                                                       tAAATGACGACTAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGcgccgc                                                                           >  2:265639/1‑250 (MQ=255)
                                                                                                                                                                acgaACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTGTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTa                                                                 <  2:213028/251‑1 (MQ=255)
                                                                                                                                                                acgaACAGTATGGCTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTACTGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCt                                                                  >  2:157159/1‑250 (MQ=255)
                                                                                                                                                                   aaaaGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGAGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGAACGACTGCTAcgg                                                              >  1:47064/4‑251 (MQ=255)
                                                                                                                                                                   aaCAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcgg                                                              >  1:47075/1‑251 (MQ=255)
                                                                                                                                                                    aCAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcggc                                                             >  1:365325/1‑251 (MQ=255)
                                                                                                                                                                         aTGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTa                                                         <  1:239714/250‑1 (MQ=255)
                                                                                                                                                                                         aCCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGAc                                        <  1:341762/251‑1 (MQ=255)
                                                                                                                                                                                                       ccAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAAc                           <  1:29972/250‑1 (MQ=255)
                                                                                                                                                                                                            aCCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGtt                     <  2:81650/251‑1 (MQ=255)
                                                                                                                                                                                                                  aaCTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGATCTCGCCTGTTTCACAGTCTGGGGAAACGCGGGTGCtgtc               >  1:253701/1‑248 (MQ=255)
                                                                                                                                                                                                                               ttAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGttgtttg   <  2:105980/250‑1 (MQ=255)
                                                                                                                                                                                                                                tAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTGCTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGttgtttgt  <  1:64592/250‑1 (MQ=255)
                                                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                
CTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGT  >  NC_000913/4128561‑4129036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑