Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,470	G→T	C329* (TGC→TGA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,470	0	G	T	100.0%	116.2 / NA	32	C329* (TGC→TGA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/17);  total (15/17)

ATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTCGCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGCGCCATCACATCGCTATGGCAGA  >  NC_000913/4123233‑4123708                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               aTAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTCGCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTaaa                                                                                                                                                                                                                                    >  2:119708/1‑250 (MQ=255)           aaaCCGCGGGCAGATTTCGTTGCTTTTTCCGGGGGGTGCTCTTTTTTCGCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACt                                                                                                                                                                                                                         <  2:208546/251‑1 (MQ=255)                               tGCTTTTTCCGTGAGGTGCTCTTTTTTCGCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCCGTCTTAAAGGTTTACTTTAAGGTAACGCGCGGTTTGATCCCCGGGTGATAAGTTCTCAGTCCATTAAACGAGAGCCACTTCCAACGTGTTGCCCCTGcc                                                                                                                                                                                                     >  2:245089/1‑250 (MQ=255)                                     ttCCGTGAGGTGCTCTTTTTTCGCCGCGAAGGTGCGGGTTGGCTGCGGCGTCCATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGa                                                                                                                                                                                                <  2:285884/250‑1 (MQ=255)                                               tGCTCTTTTTTCGCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCCGTCACGGCAGACTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATa                                                                                                                                                                                      >  2:103812/1‑250 (MQ=255)                                                               cgAAGGTGCCGGTTGGCTGCGGCGTACATAATCCCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCccg                                                                                                                                                                      >  1:312161/1‑250 (MQ=255)                                                                       ccGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTc                                                                                                                                                             <  2:181225/251‑1 (MQ=255)                                                                          gTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGt                                                                                                                                                           >  2:367005/1‑250 (MQ=255)                                                                                  cggcgTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTAATTTAAGGTAAAGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGcc                                                                                                                                                  >  2:86870/1‑250 (MQ=255)                                                                                             aTCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGtt                                                                                                                                       >  1:94775/1‑251 (MQ=255)                                                                                                 cGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGt                                                                                                                                     <  1:217753/249‑1 (MQ=255)                                                                                                  gTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTc                                                                                                                                    >  1:65175/1‑249 (MQ=255)                                                                                                       cccACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAgcggcg                                                                                                                             <  2:129800/250‑1 (MQ=255)                                                                                                           cTATCTTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGTCGGGTCTTTGACCAGTCACGGCAGTCTTAAAGGTTTAATTTAAGGTAACGCGCGTGTTTATCCCCGGATTATAAGTTCTCAGTCCATTAAACGATAGCCACTGCCACCGTGTTGCCCCTTCATTTGATCAAGCAATAACAGCCTAGCTTCCCGACCGATTTCGTAACTCGGATGCGCGATGGTTGTCAGCCGCGGATc                                                                                                                         >  2:316672/1‑251 (MQ=255)                                                                                                                             tattCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGt                                                                                                        <  1:9536/250‑1 (MQ=255)                                                                                                                              tttcgttctTCATCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGATGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATATCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTc                                                                                                       <  1:266843/249‑1 (MQ=255)                                                                                                                              attCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGGTCACAAAATTGCGTccgggcg                                                                                                      >  2:297753/1‑245 (MQ=255)                                                                                                                                 cgtgcttCAGCCCGCCATGGTACTTAATCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGAt                                                                                                    <  1:170066/244‑1 (MQ=255)                                                                                                                                      cacAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATc                                                                                              <  2:260144/249‑1 (MQ=255)                                                                                                                                                   gTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATg                                                                                 <  1:44565/251‑1 (MQ=255)                                                                                                                                                      aCTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGa                                                                               <  1:46148/250‑1 (MQ=255)                                                                                                                                                            gcggcgGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCt                                                                        <  1:286662/251‑1 (MQ=255)                                                                                                                                                                         gACCAGTAACGGCAGTCTGAAAGGTTTACTTTAAGGTAAAGCGCGTGTTGATACACGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCa                                                           <  1:316672/251‑1 (MQ=255)                                                                                                                                                                          aCCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTc                                                            >  1:113554/1‑249 (MQ=255)                                                                                                                                                                                cACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTg                                                    <  1:119708/251‑1 (MQ=255)                                                                                                                                                                                   ggCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCg                                                 <  1:356283/251‑1 (MQ=255)                                                                                                                                                                                           tAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACATCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCt                                         >  1:296662/1‑251 (MQ=255)                                                                                                                                                                                           tAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTg                                            <  2:17388/248‑1 (MQ=255)                                                                                                                                                                                                                     gtgtTGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGCGCCATcac                >  1:278106/1‑250 (MQ=255)                                                                                                                                                                                                                         tGATCCCCGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGAGCCATCACATCGc           >  1:347084/1‑251 (MQ=255)                                                                                                                                                                                                                             ccccGGATGATAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGCGCCATCACATCGCTATg       <  2:95732/251‑1 (MQ=255)                                                                                                                                                                                                                                    tgatAAGTTCTCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGCGCCATCACATCGCTATGGCaga  >  1:35335/1‑249 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               ATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTCGCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGCGCCATCACATCGCTATGGCAGA  >  NC_000913/4123233‑4123708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑