Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,051,809	G→A	E155K (GAA→AAA)	yihI →	Der GTPase‑activating protein YihI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,051,809	0	G	A	100.0%	80.9 / NA	22	E155K (GAA→AAA)	yihI	Der GTPase‑activating protein YihI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/13);  total (9/13)

CCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAGAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTACAGCGGTTGTCGCGGCGA‑CAAAAGTGGCTGCGCAACCGGCTGATGACCCGAAACGGGCATCCGCCGGTACGCCGTAGCCGCCAGAGACGCCATCGGAAGGAGTGAGCATGTCTGT  >  NC_000913/4051563‑4052052                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ccATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACGCGGCCTCTCTTATGACGATGACGAAGAAGAGGaaaaag                                                                                                                                                                                                                                                   <  2:149834/250‑1 (MQ=255)        gCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGTCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACTATGACGAATAAGAGGAAAAAGACGAGAAg                                                                                                                                                                                                                                           <  2:73163/251‑1 (MQ=255)                                              agTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACg                                                                                                                                                                                                     <  2:339796/251‑1 (MQ=255)                                                                 cACCGCAGGCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCtt                                                                                                                                                                                  <  1:33511/251‑1 (MQ=255)                                                                   ccGCAGGCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTcc                                                                                                                                                                                <  1:267694/251‑1 (MQ=255)                                                                       aGGCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGtt                                                                                                                                                                            <  2:164415/251‑1 (MQ=255)                                                                        ggCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGCACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGCAGAGCAATCCTGGGCGGATGCTAAACTGGATAGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGACGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGACGTGGGCCTTCCCGTTc                                                                                                                                                                           >  1:48317/1‑251 (MQ=255)                                                                         gCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTct                                                                                                                                                                          >  1:329105/1‑251 (MQ=255)                                                                             aGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTctgctg                                                                                                                                                                      <  1:233172/251‑1 (MQ=255)                                                                                       aCTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTc                                                                                                                                                            <  1:177162/251‑1 (MQ=255)                                                                                       aCTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTc                                                                                                                                                            <  2:337557/251‑1 (MQ=255)                                                                                          ggAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCgg                                                                                                                                                         <  2:228484/251‑1 (MQ=255)                                                                                                  aTGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGt                                                                                                                                                 <  2:145328/251‑1 (MQ=255)                                                                                                         tCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTgg                                                                                                                                          >  1:138992/1‑251 (MQ=255)                                                                                                            ggATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGgtgt                                                                                                                                       <  2:357830/251‑1 (MQ=255)                                                                                                                     gctgGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTAtt                                                                                                                                <  1:256206/249‑1 (MQ=255)                                                                                                                               cTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTAc                                                                                                                     >  1:186631/1‑250 (MQ=255)                                                                                                                                           gAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTACAGCGGTTGTcgcg                                                                                                        >  2:284703/1‑251 (MQ=255)                                                                                                                                                                    ccTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTACAGCGGTTGTCGCGGCGA‑CAAAAGTGGCTGCGCAACCgg                                                                              <  1:294592/251‑1 (MQ=255)                                                                                                                                                                                cTAAACTGGATCGGACTGATGTGTTGATGCCGAAACTCGGCCTCTCTTATGACGCTGAAGAAGAAGTGGAAAATGCCGAGAAACAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGACGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTACCGCGGTTGCCGCGGCGAACAAAA‑TGGCTGAACAACCgcagaaagacacg                                                                   >  2:337337/1‑238 (MQ=255)                                                                                                                                                                                                                                      atgacgaAGAAGAGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTACAGCGGTTGTCGCGGCGA‑CAAAAGTGGCTGCGCAACCGGCTGATGACCCGAAACGGGCATCCGCCGGTACGCCGTAGCCGCCAGAGACGCCATCGGAAGGAGTg             >  1:325942/1‑250 (MQ=255)                                                                                                                                                                                                                                              gaagaGGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTACAGCGGTTGTCGCGGCGA‑CAAAAGTGGCTGCGCAACCGGCTGATGACCCGAAACGGGCATCCGCCGGTACGCCGTAGCCGCCAGAGACGCCATCGGAAGGAGTGAGCATGTCt    >  1:85697/1‑251 (MQ=255)                                                                                                                                                                                                                                                agagGAAAAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTACAGCGGTTGTCGCGGCGA‑CAAAAGTGGCTGCGCAACCGGCTGATGACCCGAAACGGGCATCCGCCGGTACGCCGTAGCCGCCAGAGACGCCATCGGCAGGAGTGAGCATGGCTGt  >  2:353595/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATGAGCGTCTGGATGCGCTGCTGGAACGTCTGGAAGCAGGCGAAACCCTGAGTGCCGAAGAGCAATCCTGGGTGGATGCTAAACTGGATCGTATTGATGAGTTGATGCAGAAACTCGGCCTCTCTTATGACGATGACGAAGAAGAGGAAGAAGACGAGAAGCAAGAAGACATGATGCGTCTGCTGCGGGGCAACTAACGGATTGCCGCCGTGGGCCTTCCCGTTCTGCTGATAACCCTTCCGGTTATATGTTATCTGGTGTGGTTATTCGTTAAACTACAGCGGTTGTCGCGGCGA‑CAAAAGTGGCTGCGCAACCGGCTGATGACCCGAAACGGGCATCCGCCGGTACGCCGTAGCCGCCAGAGACGCCATCGGAAGGAGTGAGCATGTCTGT  >  NC_000913/4051563‑4052052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑