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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 70,659 | G→A | W91* (TGG→TGA) | araC → | DNA‑binding transcriptional dual regulator AraC |
RA | 100,946 | T→C | M61T (ATG→ACG) | murC → | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
RA | 246,747 | G→T | S12S (TCG→TCT) | yafL → | NlpC/P60 family protein YafL |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
RA | 284,535 | T→G | A445A (GCT→GCG) | yagF → | D‑xylonate dehydratase |
RA | 313,543 | G→A | intergenic (‑301/‑1748) | ykgR ← / → insE1 | putative membrane protein YkgR/IS3 element protein InsE |
RA | 480,295 | 2 bp→AA | coding (13‑14/219 nt) | hha ← | hemolysin expression‑modulating protein Hha |
RA | 690,948 | G→A | V279V (GTC→GTT) | ybeX ← | CorC‑HlyC family protein YbeX |
RA | 700,038 | G→A | H97Y (CAT→TAT) | umpH ← | UMP phosphatase |
RA | 809,217 | 2 bp→AG | coding (40‑41/1290 nt) | bioA ← | adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase |
RA | 822,527 | C→T | E405K (GAA→AAA) | clsB ← | cardiolipin synthase B |
RA | 883,396 | C→T | intergenic (‑8/‑277) | ybjG ← / → mdfA | undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter |
JC JC | 886,412 | IS2 (–) +5 bp | coding (924‑928/1209 nt) | ybjJ ← | inner membrane protein YbjJ |
RA | 968,837 | G→A | D158N (GAT→AAT) | lpxK → | tetraacyldisaccharide 4'‑kinase |
RA | 1,040,126 | G→T | L277L (CTG→CTT) | appB → | cytochrome bd‑II subunit 2 |
RA | 1,061,935 | G→A | S46N (AGC→AAC) | torD → | trimethylamine‑N‑oxide reductase‑specific chaperone |
RA | 1,098,620 | T→A | V245V (GTT→GTA) | ghrA → | glyoxylate/hydroxypyruvate reductase A |
RA | 1,111,727 | 2 bp→TT | coding (865‑866/2544 nt) | opgH → | osmoregulated periplasmic glucans biosynthesis protein H |
RA | 1,169,836 | A→G | L180P (CTG→CCG) | ldtC ← | L,D‑transpeptidase LdtC |
RA | 1,189,980 | A→G | T156T (ACT→ACC) | phoP ← | DNA‑binding transcriptional dual regulator PhoP |
RA | 1,194,922 | G→A | T10T (ACC→ACT) | rluE ← | 23S rRNA pseudouridine(2457) synthase |
RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase |
RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase |
RA | 1,196,245 | T→C | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,196,247 | A→G | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,196,277 | C→T | N385N (AAC→AAT) | icd → | isocitrate dehydrogenase |
RA | 1,196,280 | G→C | A386A (GCG→GCC) | icd → | isocitrate dehydrogenase |
RA | 1,196,283 | A→G | K387K (AAA→AAG) | icd → | isocitrate dehydrogenase |
RA | 1,196,292 | C→T | T390T (ACC→ACT) | icd → | isocitrate dehydrogenase |
RA | 1,196,304 | G→A | E394E (GAG→GAA) | icd → | isocitrate dehydrogenase |
RA | 1,196,316 | T→A | D398E (GAT→GAA) | icd → | isocitrate dehydrogenase |
RA | 1,196,325 | A→G | K401K (AAA→AAG) | icd → | isocitrate dehydrogenase |
RA | 1,196,352 | C→A | D410E (GAC→GAA) | icd → | isocitrate dehydrogenase |
JC JC | 1,223,636 | IS2 (–) +5 bp | intergenic (+187/+639) | ymgJ → / ← minE | uncharacterized protein YmgJ/Z‑ring positioning protein MinE |
RA | 1,253,002 | C→T | intergenic (+17/+83) | dhaR → / ← ycgV | DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV |
RA | 1,301,992 | A→T | N271Y (AAT→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
RA | 1,306,736 | T→G | S325A (TCC→GCC) | oppF → | murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF |
RA | 1,310,770 | A→G | H32H (CAT→CAC) | yciI ← | protein YciI |
RA | 1,337,394 | A→G | S522G (AGC→GGC) | acnA → | aconitate hydratase 1 |
RA | 1,357,202 | C→T | intergenic (‑92/+221) | sapA ← / ← ymjA | putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA |
RA | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
RA | 1,497,385 | 4 bp→CAA | coding (530‑533/1656 nt) | opgD → | glucans biosynthesis protein D |
RA | 1,584,092 | G→A | intergenic (‑133/+115) | safA ← / ← ydeP | two‑component system connector SafA/putative oxidoreductase YdeP |
JC JC | 1,590,536 | IS2 (+) +5 bp | intergenic (‑535/+314) | ydeT ← / ← hipA | fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA |
RA | 1,629,247 | C→T | I11I (ATC→ATT) | ydfZ → | putative selenoprotein YdfZ |
RA | 1,643,679 | A→T | L209Q (CTG→CAG) | ydfU ← | protein YdfU |
RA | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
RA | 1,667,460 | G→A | Q369* (CAG→TAG) | mlc ← | DNA‑binding transcriptional repressor Mlc |
RA | 1,682,459 | A→G | K101E (AAA→GAA) | rstA → | DNA‑binding transcriptional regulator RstA |
RA | 1,748,841 | C→T | R240K (AGA→AAA) | ydhT ← | uncharacterized protein YdhT |
RA | 1,767,014 | C→T | G558S (GGT→AGT) | ydiJ ← | putative FAD‑linked oxidoreductase YdiJ |
RA | 1,848,300 | 2 bp→TT | coding (376‑377/552 nt) | ydjA ← | putative oxidoreductase YdjA |
RA | 1,849,629 | T→C | L265L (TTA→CTA) | sppA → | protease IV, a signal peptide peptidase |
RA | 1,894,839 | T→C | L12P (CTC→CCC) | pabB → | aminodeoxychorismate synthase subunit 1 |
MC JC | 1,978,503 | Δ776 bp | insB5–insA5 | insB5, insA5 | |
RA | 2,040,433 | C→A | A319D (GCC→GAC) | msrP → | protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP |
RA | 2,041,278 | G→A | intergenic (+160/‑97) | msrQ → / → zinT | membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT |
RA | 2,052,885 | G→A | intergenic (+871/‑758) | yeeJ → / → shiA | inverse autotransporter adhesin/shikimate:H(+) symporter |
JC JC | 2,089,842 | IS1 (+) +9 bp | intergenic (‑129/‑146) | yefM ← / → hisL | YefM antitoxin of the YoeB‑YefM toxin‑antitoxin pair and DNA binding transcriptional repressor/his operon leader peptide |
RA | 2,104,024 | G→T | I162I (ATC→ATA) | wbbK ← | putative glycosyltransferase WbbK |
RA | 2,111,259 | Δ1 bp | coding (718/900 nt) | rfbD ← | dTDP‑4‑dehydrorhamnose reductase |
RA | 2,142,598 | G→A | L117L (CTC→CTT) | udk ← | uridine/cytidine kinase |
RA | 2,155,197 | G→A | Q394Q (CAG→CAA) | mdtA → | multidrug efflux pump membrane fusion protein MdtA |
RA | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
RA | 2,173,448 | C→T | intergenic (‑575/+834) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
JC JC | 2,176,844 | IS4 (–) +13 bp | coding (349‑361/855 nt) | gatY ← | tagatose‑1,6‑bisphosphate aldolase 2 |
RA | 2,233,609 | C→T | Q4* (CAG→TAG) | yeiS → | DUF2542 domain‑containing protein YeiS |
JC JC | 2,238,948 | Δ1 bp :: IS186 (–) +6 bp :: Δ2 bp | coding (337‑342/1521 nt) | mglA ← | D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit |
RA | 2,242,247 | C→T | G241R (GGG→AGG) | yeiB ← | DUF418 domain‑containing protein YeiB |
RA | 2,247,134 | A→C | Y467D (TAC→GAC) | lysP ← | lysine:H(+) symporter |
RA | 2,307,733 | C→T | A295T (GCG→ACG) | yojI ← | ABC transporter family protein/microcin J25 efflux protein |
RA | 2,310,878 | 2 bp→TT | coding (656‑657/1056 nt) | ftp ← | FAD:protein FMN transferase |
RA | 2,404,402 | G→A | S71L (TCG→TTG) | nuoB ← | NADH:quinone oxidoreductase subunit B |
RA | 2,405,840 | +T | coding (802/939 nt) | lrhA ← | DNA‑binding transcriptional dual regulator LrhA |
RA | 2,446,592 | C→A | P294P (CCG→CCT) | aroC ← | chorismate synthase |
RA | 2,485,636 | Δ1 bp | coding (1263/3594 nt) | evgS → | sensor histidine kinase EvgS |
RA | 2,485,638 | T→A | L422* (TTA→TAA) | evgS → | sensor histidine kinase EvgS |
RA | 2,522,369 | G→A | P37S (CCG→TCG) | xapR ← | DNA‑binding transcriptional activator XapR |
MC JC | 2,558,699 | Δ6,790 bp | intZ–[eutA] | intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA] | |
RA | 2,587,112 | C→T | A461V (GCA→GTA) | narQ → | sensor histidine kinase NarQ |
RA | 2,668,977 | C→T | intergenic (‑81/‑55) | hcaR ← / → hcaE | DNA‑binding transcriptional dual regulator HcaR/putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha |
RA | 2,702,363 | G→A | A36V (GCC→GTC) | recO ← | recombination mediator protein RecO |
JC | 2,765,463 | (TATGGCAC)6→7 | intergenic (‑310/+50) | abpA ← / ← yfjM | anti‑bacteriophage protein/protein YfjM |
RA | 2,774,947 | G→A | G544S (GGT→AGT) | yfjW → | uncharacterized protein YfjW |
RA | 2,867,311 | G→T | R81S (CGT→AGT) | rpoS ← | RNA polymerase sigma factor RpoS |
RA | 2,880,635 | T→C | L138L (TTA→TTG) | casD ← | type I‑E CRISPR system Cascade subunit CasD |
RA | 2,926,772 | C→A | G155G (GGC→GGA) | ppnN → | nucleotide 5'‑monophosphate nucleosidase |
RA | 2,936,855 | T→G | A424A (GCT→GCG) | fucI → | L‑fucose isomerase |
RA | 2,937,210 | C→T | L543F (CTC→TTC) | fucI → | L‑fucose isomerase |
RA | 2,938,548 | A→T | T361S (ACG→TCG) | fucK → | L‑fuculokinase |
RA | 3,025,601 | (T)5→4 | coding (1251/1401 nt) | xanQ → | xanthine:H(+) symporter XanQ |
RA | 3,070,858 | T→G | K129N (AAA→AAC) | fbaA ← | fructose‑bisphosphate aldolase class II |
RA | 3,207,549 | Δ1 bp | coding (179/606 nt) | ttdB → | L(+)‑tartrate dehydratase subunit beta |
RA | 3,279,276 | C→G | F121L (TTC→TTG) | kbaZ → | putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone |
RA | 3,296,481 | G→A | V25M (GTG→ATG) | dolP → | division and outer membrane stress‑associated lipid‑binding lipoprotein |
RA | 3,324,965 | G→T | intergenic (‑54/+36) | folP ← / ← ftsH | dihydropteroate synthase/ATP‑dependent zinc metalloprotease FtsH |
RA | 3,326,537 | C→A | S133S (TCG→TCT) | ftsH ← | ATP‑dependent zinc metalloprotease FtsH |
RA | 3,329,743 | G→T | D261Y (GAT→TAT) | dacB → | peptidoglycan DD‑endopeptidase DacB |
RA | 3,388,041 | T→G | T50P (ACG→CCG) | aaeB ← | aromatic carboxylic acid efflux pump subunit AaeB |
RA | 3,411,689 | G→T | E13* (GAA→TAA) | yhdJ → | DNA adenine methyltransferase |
RA | 3,433,900 | C→T | L71L (CTA→TTA) | def → | peptide deformylase |
RA | 3,439,485 | C→T | E9K (GAG→AAG) | yhdN ← | DUF1992 domain‑containing protein YhdN |
RA | 3,474,272 | C→T | R94H (CGT→CAT) | rpsL ← | 30S ribosomal subunit protein S12 |
RA | 3,474,424 | T→G | K43N (AAA→AAC) | rpsL ← | 30S ribosomal subunit protein S12 |
RA | 3,486,502 | C→T | T128I (ACT→ATT) | crp → | DNA‑binding transcriptional dual regulator CRP |
RA | 3,486,538 | T→C | V140A (GTG→GCG) | crp → | DNA‑binding transcriptional dual regulator CRP |
RA | 3,511,466 | C→T | V293V (GTG→GTA) | yhfZ ← | putative DNA‑binding transcriptional regulator YhfZ |
RA | 3,520,507 | G→A | P66L (CCC→CTC) | hofQ ← | DNA utilization protein HofQ |
RA | 3,555,047 | G→C | V655L (GTC→CTC) | malT → | DNA‑binding transcriptional activator MalT |
RA | 3,560,362 | G→A | intergenic (+496/+260) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
RA | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
RA | 3,647,428 | C→T | A377V (GCG→GTG) | gor → | glutathione reductase (NADPH) |
RA | 3,707,947 | C→A | intergenic (‑242/+669) | dppA ← / ← proK | dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro |
RA | 3,719,429 | G→A | intergenic (‑385/‑49) | yiaF ← / → yiaG | DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG |
RA | 3,725,176 | T→G | E48A (GAG→GCG) | glyQ ← | glycine‑‑tRNA ligase subunit alpha |
RA | 3,729,440 | 2 bp→TT | noncoding (105‑106/160 nt) | xylZ ← | small RNA XylZ |
RA | 3,730,560 | C→T | W69* (TGG→TAG) | xylA ← | xylose isomerase |
RA | 3,770,539 | C→T | V281M (GTG→ATG) | yibH ← | inner membrane protein YibH |
RA | 3,773,173 | Δ1 bp | coding (893/1914 nt) | mtlA → | mannitol‑specific PTS enzyme IICBA component |
RA | 3,773,176 | C→T | S299F (TCT→TTT) | mtlA → | mannitol‑specific PTS enzyme IICBA component |
RA | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
RA | 3,925,834 | C→T | intergenic (‑201/+178) | mnmG ← / ← mioC | 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG/flavoprotein MioC |
RA | 3,926,991 | G→A | L5L (CTG→TTG) | asnC ← | DNA‑binding transcriptional dual regulator AsnC |
RA | 3,966,530 | 2 bp→TT | coding (114‑115/1260 nt) | rho → | transcription termination factor Rho |
RA | 4,003,502 | G→T | P226Q (CCG→CAG) | rarD ← | putative transporter RarD |
JC JC | 4,123,617 | IS2 (–) +5 bp | coding (836‑840/1026 nt) | cytR ← | DNA‑binding transcriptional repressor CytR |
RA | 4,133,311 | G→A | P232P (CCG→CCA) | metF → | 5,10‑methylenetetrahydrofolate reductase |
RA | 4,150,418 | A→G | intergenic (‑153/+29) | eptC ← / ← ppc | phosphoethanolamine transferase EptC/phosphoenolpyruvate carboxylase |
RA | 4,193,996 | T→G | D70A (GAT→GCT) | thiE ← | thiamine phosphate synthase |
RA | 4,217,310 | A→G | S68G (AGC→GGC) | aceA → | isocitrate lyase |
RA | 4,223,638 | T→C | intergenic (‑10/‑190) | iclR ← / → metH | DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
RA | 4,325,644 | C→T | S33N (AGC→AAC) | yjdN ← | PF06983 family protein YjdN |
RA | 4,331,890 | C→T | L463L (CTC→CTT) | proP → | osmolyte:H(+) symporter ProP |
RA | 4,437,890 | C→T | Q62* (CAG→TAG) | ytfI → | protein YtfI |
RA | 4,473,900 | C→T | G52R (GGA→AGA) | bdcA ← | c‑di‑GMP‑binding biofilm dispersal mediator protein |
RA | 4,475,493 | T→C | F19F (TTT→TTC) | yjgL → | protein YjgL |
RA | 4,476,605 | G→A | S390N (AGC→AAC) | yjgL → | protein YjgL |
RA | 4,489,016 | C→T | E350K (GAA→AAA) | yjgR ← | DUF853 domain‑containing protein YjgR |
RA | 4,510,238 | T→G | intergenic (+445/+452) | insO → / ← fecE | IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit |
RA | 4,532,409 | T→G | intergenic (‑758/+28) | sgcX ← / ← yjhP | putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP |
RA | 4,546,601 | C→T | I502I (ATC→ATT) | fimD → | type I fimbriae usher protein |
RA | 4,620,610 | G→A | L336L (CTG→CTA) | deoB → | phosphopentomutase |
RA | 4,636,925 | G→C | R77P (CGC→CCC) | creC → | sensory histidine kinase CreC |
RA | 4,638,240 | G→A | L21L (TTG→TTA) | creD → | putative inner membrane protein CreD |
JC JC | 4,640,418 | IS30 (–) +2 bp | coding (17‑18/141 nt) | yjjY → | uncharacterized protein YjjY |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1196356 | 1211454 | 15099 | 12 [10] | [10] 11 | [icd]–mcrA | [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA |
* | * | ÷ | NC_000913 | 2822727 | 2829585 | 6859 | 11 [10] | [4] 13 | [recA]–[srlR] | [recA],pncC,mltB,srlA,srlE,srlB,srlD,gutM,[srlR] |
* | * | ÷ | NC_000913 | 3423879–3424234 | 3424432–3424238 | 5–554 | 11 [9] | [10] 11 | [rrlD] | [rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 244134 = | 75 (1.000) | 117 (1.610) | 95/468 | 0.0 | 61.7% | intergenic (+13/‑193) | gmhA/yafJ | D‑sedoheptulose 7‑phosphate isomerase/putative glutamine amidotransferase YafJ |
? | NC_000913 | = 377392 | 73 (1.010) | intergenic (+81/+143) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 256107 | 143 (1.900) | 46 (0.630) | 45/470 | 0.6 | 23.2% | coding (131/459 nt) | gpt | xanthine‑guanine phsophoribosyltransferase |
? | NC_000913 | 371382 = | 167 (2.290) | coding (144/867 nt) | mhpC | 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase | |||||
* | ? | NC_000913 | = 258675 | NA (NA) | 57 (0.760) | 48/486 | 0.5 | 82.6% | noncoding (1/768 nt) | IS1 | repeat region |
? | NC_000913 | = 1301058 | 12 (0.160) | intergenic (+511/‑124) | insH21/oppA | IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | = 366180 | 4 (0.050) | 216 (2.870) | 174/486 | 0.0 | 98.4% | coding (126/3075 nt) | lacZ | beta‑galactosidase |
? | NC_000913 | 366274 = | 3 (0.040) | coding (32/3075 nt) | lacZ | beta‑galactosidase | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 33 (0.450) | 33/478 | 1.1 | 100% | intergenic (+253/‑69) | ychE/insH21 | MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 1301050 = | 14 (0.190) | 45 (0.600) | 40/486 | 0.8 | 76.3% | intergenic (+503/‑132) | insH21/oppA | IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1978503 = | NA (NA) | noncoding (768/768 nt) | IS1 | repeat region | |||||
* | ? | NC_000913 | = 1651549 | NA (NA) | 38 (0.500) | 38/486 | 0.9 | 43.2% | intergenic (+1564/+1179) | ydfD/ynfP | lysis protein/protein YnfP |
? | NC_000913 | = 3938771 | 50 (0.660) | coding (545/993 nt) | rbsR | DNA‑binding transcriptional dual regulator RbsR | |||||
* | ? | NC_000913 | 3186096 = | NA (NA) | 30 (0.400) | 28/486 | 1.4 | 35.3% | noncoding (1/1331 nt) | IS2 | repeat region |
? | NC_000913 | 3938768 = | 55 (0.730) | coding (542/993 nt) | rbsR | DNA‑binding transcriptional dual regulator RbsR |