| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,358,859 | 0 | T | C | 96.8% | 191.5 / NA | 63 | Y110C (TAT→TGT) | puuP | putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (30/30); minor base A (2/0); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
AGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATC > NC_000913/1358614‑1359101 | aGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑Tcc < 1:365150/251‑1 (MQ=255) aCGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCcag < 1:503804/251‑1 (MQ=255) cGGTGCAAACGCATTCTCCTTTATGCAGTCCAGGAACTATCAGGAAGATAAACACCGCCATGATGGAGATTTGCACCAGTACAATCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATGCGCGGCGGTTAAAATGGCGACGAATGTTACCACCCACACCCACGGCCGCACTTCCGGGAAGAGGGCGGAGAGAGAGA‑TGTTCGCCAACAAGACGTGAATCATCGGCAAAAAGAGACAA‑TCcagc < 2:32277/251‑1 (MQ=255) gTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCcagcag < 1:274000/251‑1 (MQ=255) tctcCTTGATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTGGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTGGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGc < 2:759207/250‑1 (MQ=255) aGTCCCTGAACTACCAGGAAGATAAACTCCACCATGATGTAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACTCTCTGCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATcc < 2:262157/251‑1 (MQ=255) cccTGAACTACCAGGAAGATAAACCCCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGAc < 2:326012/251‑1 (MQ=255) cccTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGAc < 1:244609/251‑1 (MQ=255) gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTGCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTg < 1:116257/250‑1 (MQ=255) aaCTACCAGGAAGATAAACACCACCATGCTGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTTAAATGGCGACGAAGGGTACCACCCCAACCCACGACGGCACTTCCGGGAAGAGGGCGGGGAGATAGA‑TTTTCGCCAACAAGAAGATAATCCTCGGCAAAAAGAGAAAA‑TAACGCAGCGGTGACCAGCCCACCATaaaatcgacgggcg > 1:661227/1‑240 (MQ=255) cccAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGtt < 2:285500/250‑1 (MQ=255) cggAAGTTAAACACCACCACTAGGGAAATTTGCACCTGTACAAACAGGGTATTGAATTGAGCCACCTGGTTTACGCTCTTCAGTATCGAGGCGGTTAAAAGGGCGTCGAAGTTTACCACCCCCACTCACGGCGTCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTGAAGCACCGGCAAAAAGAGACAA‑TCCAGCTGCGGTGACCAGCCGACCCTACAACCGACGTGCTGGTTAAt < 2:789076/250‑1 (MQ=255) aGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGAGCGGGGTAATCGAc > 1:605987/1‑251 (MQ=255) aaaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACtttt > 1:289961/1‑251 (MQ=255) aaaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACtttt < 1:647463/251‑1 (MQ=255) gatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGCGAAGGTTACCACCCACACCCACGGCGGCACCTCCGGGGAGAGGGCGGAGAGGTAGA‑TTTTCGCCCACAAGACGTTTATCATCGGGCAAAAGAGACAA‑TCCAGCAGCGCTGACCAGCCGACCAAAAATCCGACGTGCGGGGTAATCGGCTTTTACGCGCAggca > 2:186015/1‑248 (MQ=255) gatgGAGATTTGCACCAGTACAAACAGGGGATTGAAGTTAGCGCCCAGGTTGACGCTCGTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGta < 2:449721/250‑1 (MQ=255) ggAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAgg < 1:655370/251‑1 (MQ=255) tcACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGGCGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTCCCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGACGGAGATATAGA‑GTTTCGCCAACAAGACGTGAATCATCGGCAAAAAGAGACCA‑TCCAGCAGCGATGACCAGCCGATCATAAATTCGACGTGCAGGTTAATCGTCTTTTGCTCGTAGGTATAGGCCGAAcc < 2:330587/249‑1 (MQ=255) tACAACCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGATAAAATGGCGGCGAAGGGTACAACCCACACCCACGGCGGGACTTCCGGGAAGAGGGGGGAGAGATAGA‑TTTTTGACAACAAAACGTTAATCATCGGCAAAAAGAGACAA‑TACAGCAGCGGTGAACACCCGACCATAAAACCGACGAGCGGGGTAATAGACTATTGCGCGTAGGTATAggacggagcagccaccg > 1:29446/1‑236 (MQ=255) aCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGGGCGGGGTAATCGACTTTTGCGCGGAGGGATAGGCCGAACCGGCCTCCGG‑AAAc > 1:184555/1‑251 (MQ=255) ggTATTGAAGGTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGc < 1:330556/251‑1 (MQ=255) gAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGGGACGAAGGGTACCACCCCCCCCCACGGCGGCCCTTCCGGGGAGAGGGGGGGGAGATAAA‑TTTTTGCCAACAAGACGTTAATCATCCGCAAAAAGAGACAA‑ACCAGCAGCGGCGACCCGCCGCCCATAAAACCAAACTGCGGGGTAATCGACTTTTGCGCGTAGGTAAAGGCCGGACCCGCCTCCGG‑ACACAGGCGCAccc > 2:380732/1‑250 (MQ=255) aaGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTTAAATGGGGGCGAAGGTTACCACCCCCACCCACGGGGGGACTTTCGGGAAGAGGGAGGGGAGATAGA‑TTTTTGCCAACAAGAAGGTAATCATCGGCAAAAAGAGAAAA‑ACCAGCAGCGATGACCAGCCGACCAAAAATCCGAAGTGCGGGTTAATAGACTTTTGTGCGTAGGTATAGGCCGAACCGGCCTCCGG‑GAACCGGCGAAcc > 2:731425/1‑249 (MQ=255) aGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAACATGGCGGCGAAGGTTAACACCCACACCCCCGGCGGCACTTCCGGGGAGAGGGCGGAGAGATAGA‑TTTTTGCCCACAAGACGGTAATCATCGGCCAAAAGAGACAA‑ACCAGCAGCGGTGACAAGCCGACCATAAATCCGACGTGCGGGTTAAACGACTTTTGCGCGAAGGGATAGGCCGAACCGGCCTCCCG‑AAACTGGCGAACCAGGTTGc > 1:697199/1‑251 (MQ=255) aCCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGAAGGGTACCACCCACCCCCACCGCGGCCCTTCCGGGCAGGGGGCGGAGAGATAGA‑TTTTCGCCAACCAAACGCTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTTCGCGTAGGGATAGGCCGAACCCGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGtt > 1:415922/1‑250 (MQ=255) cAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCAAACCCACGGCGGCACTTCCGGGAAGAGGGGGGGGAGATAGA‑TTTTCGCCAAAAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCAAAAAACCGACGTGCGGGGTAATCGACTTTTTCGCGTAGGGATAGGCCGAACCGGCCTCCGG‑GAACTGGCGAAACAGTTTGCCGTAgg > 1:1740/1‑250 (MQ=255) ggTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCCCACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCCACGTGCGGGTTAATCGACTTTTTCGCGTAGGTATAGGCCGAACCGGCCTCCCG‑AAACTGGCGAACCAGTTTGCCGTAGCTg > 1:332649/1‑251 (MQ=255) aCGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCATCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGc < 1:118017/251‑1 (MQ=255) gATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGA‑TTTTCGCCAACACGACGTTAAACATCGGCAAAAAAAGACAA‑ACCAGCAGCGATGAACAGCCGACCATAAATCCGACGGGCGGGGTAATCGACTTTTTCGCGTAGGTATAGGCCGAACCGGCCTCCGGGAAAC‑GGCGAACCAGTTTGCCGTAGCTGAAAGCGGTAAAAAGc > 1:698217/1‑251 (MQ=255) gATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGc < 1:304662/251‑1 (MQ=255) aTTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGca < 1:441298/251‑1 (MQ=255) cggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAccc < 1:731425/251‑1 (MQ=255) cggTTAAAATAGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATCGGCCGAACCGGCATCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgcc > 2:803673/1‑251 (MQ=255) ggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGATAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGGTTGCCGTAGCTTATAGCGGTAAACAGCACACCcgcc > 2:752021/1‑250 (MQ=255) gatAAAATGGCGACGAAGGTTACCACACACACCCACGGCGGCACTTACGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCCTAAATCCGACGTGCGGGTTAATCGCCTTTTGCGCGTAGGGATAGGCCGAACCGGCCGCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAACGGTGAActgaaacccccccc > 2:19772/3‑237 (MQ=255) aaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcgccc > 2:628021/1‑250 (MQ=255) aaGGTTACCACCCACACCCACGGCGGCACTTACGGGAGGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAgg > 1:217998/1‑250 (MQ=255) ggTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGcac < 2:59151/225‑1 (MQ=255) ggTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGcac > 1:59151/1‑225 (MQ=255) gTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGAAAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGc > 1:721777/1‑251 (MQ=255) ttACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGcc > 1:793630/1‑251 (MQ=255) cccacccacCCCCACGGCGGCACTCCCGGGAAGAGGGCGGAGAGATAGA‑TTTCCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGTTAAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCgg < 1:112989/250‑1 (MQ=255) ccccccacccccccGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTACGCAAAAAAGACGTTAATCATCGGAAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTTATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGa < 1:717468/248‑1 (MQ=255) cccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGCCGCCGGAACGTGACCg > 2:168486/1‑251 (MQ=255) cacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTc > 2:136583/1‑251 (MQ=255) cggcACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACAGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTa > 1:638651/1‑250 (MQ=255) gcACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAAAAAGACGTTAATCACCGGAAAAAAGAGACAA‑TCCAGCAGCGATGCCCAGCCGACAATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGTTATAGGCCGACCCGGCCCCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATg < 1:380732/251‑1 (MQ=255) cgtCCGGGAAGAGGGCGGAGAGTAAAA‑TTGTCGCCACAAAGCCGTAAACAACCGGAAAAAAGAGACAA‑CCCAGAAGCGATGCCCAGCCGACCATAAATCCGACGTGGGGGTTAATCGCCTTTTGCGGGTAGGTAAAGGCCGAACCGGCCTCCGG‑AACCGGGCGAACCAGTTTGCCGTAGGTGAGAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCg < 2:332649/249‑1 (MQ=255) ccGGGAAGAGGGCGGAAAAATAGA‑TTTCCGCCAACAAGACGTTAATCATCGGAAAAAAGAGACAA‑TCCAGCAGCGAGGACCAGCCGACAAAAAATCCGACGTGCGGTTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGG‑AAACGGGCGAACCAGTTTGCCGTAGCGGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGgag < 1:651657/251‑1 (MQ=255) gggAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGAc > 2:242128/1‑251 (MQ=255) gAAGAGGGGGGAGAAATAAA‑TTTCCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGCCCAGCCGCCCATAAACCCGACGGGCGGGTTAATCGACTTTTGCGCGTGTGTATAGGCCGAACCGGCCCCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCTCTCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACaa < 1:541039/251‑1 (MQ=255) aaGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAAt > 1:570403/1‑251 (MQ=255) aaGAGGGCGGAGAGA‑AGAATTTCCGCCAACAAGACGGTATACAGCGGCAAAAA‑AGATAAACCCAGCAGCTATGACCAGCCGACGATAAACCCGGCGTGCGGGTTAGTCTACTTTTGCGCGTAGGTATGGGCGGAACCGGCCTCGTG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGAAAACAACGCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAAt < 1:791131/251‑1 (MQ=255) gggCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAC‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATAcc > 1:336023/1‑250 (MQ=255) aagtttttCGCCAAAAAGACTTTAATCAACGGAAAAAAAGGACAC‑TCCACCAGCGATGACCGTCCGACCAAAAATCCGCCGTGCGTGTTAATCGCTTTTTGCGCGTAGTTATAGGCCGACCCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCTTAGCGTATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCaaa < 1:590334/247‑1 (MQ=255) cGCCAACAAGACGTTAATCATCGGCAACAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTCGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGAATCAAATACGGTc > 1:625111/1‑250 (MQ=255) caaGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCg > 1:691845/1‑251 (MQ=255) aCGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGAGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGAAAACAGCACTCCCGCCAGCTCCAGCAAATAGGACGCCGAAAAGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAAATCGGTCATCGGCGTg > 1:609685/1‑249 (MQ=255) atcatcGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCACATAGGACGCCGGACCGTGACCGTCGCTAACGCCGGAGACACTACCAAAAGTATCAAATACGGCCATCGGCGTGAGAAAGGc > 2:97369/1‑251 (MQ=255) aaaaGATACAA‑TCAAGCAGCGATGACCACCCGACCATAAATCCGCCGTGCGGTTTAACCGATTTTTGCGCTTAGTTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGACCCATTTTGCCGTAGCTAATAGCGGTAAACAGCACACCCGCCATCGCCAGCAAATAGGACGCCGGAACGTGACCGGCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcatc < 1:475197/251‑1 (MQ=255) aaaaGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCacac > 2:502594/1‑144 (MQ=255) aaaaGAGACAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCacac < 1:502594/144‑1 (MQ=255) | AGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGA‑TTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAA‑TCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGG‑AAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATC > NC_000913/1358614‑1359101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |