Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	234.6 / NA	71	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/36);  total (35/36)

CCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337151‑1337638                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ccAAGTGGGCTGATGGCCGCAGTCTTGCTGGCGAAAAAAGCCGTAACTCTTGGCCTCAAACGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAttt                                                                                                                                                                                                                                                <  2:596543/251‑1 (MQ=255)        tgCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCCCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTc                                                                                                                                                                                                                                         >  1:67246/1‑251 (MQ=255)           tGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTCACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                                                                                                                                                                                      >  2:206220/1‑251 (MQ=255)                   gCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                                                                                                                                                                              >  1:228407/1‑251 (MQ=255)                        cTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATAGGGTTGTACCACCTTAATTGGTAACTCTGGGCCGCTGCCCGACCCTACCGAAACGGCATCCAAAAAAGGCGTTTTACCCGTCGGGCCGGTGCTGTCCg                                                                                                                                                                                                                         >  1:7298/1‑251 (MQ=255)                         ttGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCgg                                                                                                                                                                                                                        >  1:536632/1‑251 (MQ=255)                          tGCTGGCGAAAAAAGCCGTACCTCTGGGCCTCAAGCGGCACCCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCGCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCg                                                                                                                                                                                                                         <  2:475764/249‑1 (MQ=255)                                  aaaaaaGCCGTACCTCTGGGCCTCAAGCGGCAACCATGGGCCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTAGCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTaa                                                                                                                                                                                                               <  2:624625/251‑1 (MQ=255)                                  aaaaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTaa                                                                                                                                                                                                               <  2:42368/251‑1 (MQ=255)                                    aaaaGCCGGAACTTTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACt                                                                                                                                                                                                             <  2:682697/251‑1 (MQ=255)                                          cGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAg                                                                                                                                                                                                       <  1:746268/251‑1 (MQ=255)                                                         cAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatcc                                                                                                                                                                                          <  1:122391/249‑1 (MQ=255)                                                          aaGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCt                                                                                                                                                                                       >  2:298314/1‑251 (MQ=255)                                                           aGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTg                                                                                                                                                                                      >  2:624281/1‑251 (MQ=255)                                                            gCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTgg                                                                                                                                                                                     <  2:242027/251‑1 (MQ=255)                                                                      tgtgTCAAAGCGTCGCTTGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTCTCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaccta                                                                                                                                                                            >  2:812480/4‑250 (MQ=255)                                                                       gggTAAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTGTCTGATTATCTGGCAAAAGCGAAACTGACCCC‑GTAGCTCGTCGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATGAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTTACCTGCAACCTTAACTTTGAAGGCCGCATCCATCCGCTGGTTAAaactaac                                                                                                                                                                          <  1:319288/251‑1 (MQ=255)                                                                           cAAAGCGTCGCTGGCACCGGGGTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCGCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggc                                                                                                                                                                      <  1:783048/251‑1 (MQ=255)                                                                              aGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctgg                                                                                                                                                                   >  2:69545/1‑251 (MQ=255)                                                                                 gTCGCTGGCTCCGGTATCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTGGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCt                                                                                                                                                                <  2:346718/251‑1 (MQ=255)                                                                                 gTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGccc                                                                                                                                                                >  2:400108/1‑250 (MQ=255)                                                                                 gTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCt                                                                                                                                                                <  1:198952/251‑1 (MQ=255)                                                                                      tgtcACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGGGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGATGGCCTcgccg                                                                                                                                                           >  2:447395/4‑251 (MQ=255)                                                                                                  cGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGtt                                                                                                                                               <  1:708344/251‑1 (MQ=255)                                                                                                    aatgTCGTTTCTGTTTATCTGTCAAAAGCGTAA‑TTTCACCCTTATGTCGACTAACGGGGGTTTATCCTTGTGGGATACGGTTGTACCACCGTTTTTGTTAACTCTGGGCCTCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCTTCGGTGCGGTGCTGGCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTTGTTGc                                                                                                                                             <  2:816499/248‑1 (MQ=255)                                                                                                           tttCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTGGCCTATgcg                                                                                                                                      >  2:458171/1‑251 (MQ=255)                                                                                                              cTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGCACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAATACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTg                                                                                                                                   >  1:89504/1‑251 (MQ=255)                                                                                                                gATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGATCTTGGGGTTAACCGTGTGGGATACGTTTGGACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCTTCGCCGCCGCTGGTGGTTGCCTATGCGCTg                                                                                                                                   <  2:619671/249‑1 (MQ=255)                                                                                                                gATTATCTGGCAAAAGCGAAACTGACACC‑GTAGCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGc                                                                                                                                 <  1:701810/251‑1 (MQ=255)                                                                                                                   tATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCggg                                                                                                                              >  1:642233/1‑251 (MQ=255)                                                                                                                      cTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑aa                                                                                                                           >  2:754410/1‑250 (MQ=255)                                                                                                                          aaaaaGCGAAACTGTCACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATg                                                                                                                      <  1:88789/250‑1 (MQ=255)                                                                                                                                     cTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTATGGGATACGGTTGAACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAACAAGGCGATTTAACCGTCGGAGCGGTGCTGTCCGGCAACCGTAACTTTAAAAGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCAGACGCTGGTGGTTGCCTATGCGCTGGCGGG‑GAATTTGAATTTCAACCt                                                                                                           >  2:65113/1‑251 (MQ=255)                                                                                                                                      tGACACC‑GTCTCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCCCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGCAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGAAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAACCTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGACCTGGCTGG‑GAATATggacttcaacatg                                                                                                          >  2:782486/1‑239 (MQ=255)                                                                                                                                      tGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTg                                                                                                          >  1:318443/1‑251 (MQ=255)                                                                                                                                       gacccc‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTTGCCCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTg                                                                                                          <  2:143545/246‑1 (MQ=255)                                                                                                                                         cacC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTAGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAc                                                                                                                                                                                                              <  1:321319/149‑1 (MQ=255)                                                                                                                                         cacC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTAGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAc                                                                                                                                                                                                              >  2:321319/1‑149 (MQ=255)                                                                                                                                          acC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGAACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTCACCGACGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTCTCCATCCGCTGGATAAAACTAACTGGCTGGCCTCACCGCCCCTGGGGGCTGCCTATGCGCTGGCGGG‑AAAAATGGATAACACCCTGGCtt                                                                                                      >  2:648319/1‑251 (MQ=255)                                                                                                                                           cc‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGGAAAT‑TGAATATCAACCTGGGTTc                                                                                                     >  2:669227/1‑251 (MQ=255)                                                                                                                                                   tcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCt                                                                                              >  1:410226/1‑251 (MQ=255)                                                                                                                                                    cgacgaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAg                                                                                                 <  1:164000/247‑1 (MQ=255)                                                                                                                                                         aaCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGc                                                                                        <  1:298314/251‑1 (MQ=255)                                                                                                                                                               ggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGAt                                                                                  <  1:337564/251‑1 (MQ=255)                                                                                                                                                                gTTTAACCTTGTGGGATACGGTTGTACCACCTGTAGTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATc                                                                                 <  2:318443/251‑1 (MQ=255)                                                                                                                                                                  ttAACCTGGTGGTATACGGTTGTTCCACCGGTATTGGTAACTCTGGGCCGCTGCCCGATCCTACGGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AATTATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCg                                                                                <  1:771656/250‑1 (MQ=255)                                                                                                                                                                   tAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTCTGCGATGGCGGG‑GAATATGCATATCAACCTGGCTTTTGAGGCTATCGGCCacggttgg                                                                               >  2:258252/1‑243 (MQ=255)                                                                                                                                                                    aaCCTTGTGGGATACGGTTGTACCCTCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGGCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCaa                                                                             <  1:543784/251‑1 (MQ=255)                                                                                                                                                                     aCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCaaa                                                                            <  1:548043/251‑1 (MQ=255)                                                                                                                                                                       cTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAACCTATCCGCCATGATCGCACAgg                                                                          >  2:313171/1‑251 (MQ=255)                                                                                                                                                                                    gTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAAACTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGAGATCCGGTTTAt                                                             >  1:406572/1‑251 (MQ=255)                                                                                                                                                                                                 tATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGGTTATCTGAAAGATATCt                                                >  1:279005/1‑251 (MQ=255)                                                                                                                                                                                                  aTTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCt                                                <  1:400108/250‑1 (MQ=255)                                                                                                                                                                                                      gTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGcc                                            >  1:213915/1‑250 (MQ=255)                                                                                                                                                                                                      gTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCa                                           <  1:812480/251‑1 (MQ=255)                                                                                                                                                                                                        aaCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCCAAGGCGATCCGGTTTTTCTGAAAGATATCTGGCCATc                                         >  2:777231/1‑251 (MQ=255)                                                                                                                                                                                                        aaCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATc                                         <  1:283970/251‑1 (MQ=255)                                                                                                                                                                                                         aCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATATGGCCATCg                                        >  1:11782/1‑251 (MQ=255)                                                                                                                                                                                                            ctGGGCCGCTGCCCGATCCTACCGAAACGGAAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCgg                                       <  2:684022/249‑1 (MQ=255)                                                                                                                                                                                                             tGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGAACGCAAAGGCGATCCGGATTATCTGAAATATCTCTGGCCATCGGcac                                    >  1:567138/1‑251 (MQ=255)                                                                                                                                                                                                             ggggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑GAATATTAATATCCACCCGGGTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGGTTATCTGAAAGATATCTGGCCATCGGcc                                     >  2:597545/2‑249 (MQ=255)                                                                                                                                                                                                                      gCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAAtt                            <  2:228407/250‑1 (MQ=255)                                                                                                                                                                                                                      gCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAAtt                            <  1:206220/250‑1 (MQ=255)                                                                                                                                                                                                                         cGATCCTATCGAAACGGAAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGccc                        <  1:447395/251‑1 (MQ=255)                                                                                                                                                                                                                         cGATCCTAGCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGccc                        <  1:528639/251‑1 (MQ=255)                                                                                                                                                                                                                          gATCCTATCGAAACGGCAATCAAAAAAGGCTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCg                       <  1:648319/251‑1 (MQ=255)                                                                                                                                                                                                                           aTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGAACGCCAAGGCGATCCGGGTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTTCCCgg                      >  2:117820/1‑250 (MQ=255)                                                                                                                                                                                                                            tCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGACTATCGGCCATGATCGCAAAGGCGATCCGGGTTTTCTGTAAGATATCTTGCCCTCGGCACAAGAAAATTCCCGTg                     >  2:570589/1‑251 (MQ=255)                                                                                                                                                                                                                               tATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCgg                  >  1:17844/1‑251 (MQ=255)                                                                                                                                                                                                                                 acGAAACGGCAAGCAAAAAAGGCGATTTAACCGGCGGTGCGGTGCTGTCCGGCAACCGTACCTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTa                <  2:222806/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaCGGCAATAAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCCCAAAGGCGATCCGGGTTATCTTAAAGATATCTGGCCCTCGGCACAAGAAATTTACCGTGCGGGAGaa             >  2:433036/1‑250 (MQ=255)                                                                                                                                                                                                                                               aaaaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTATCcac  >  1:781979/1‑251 (MQ=255)                                                                                                                                                                                                                                               aaaaaagGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTAGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGCATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGCTCCGGTTTATCTGTAAGATATCTGGCCATCGGCCCCAGAAATTGCCCGTTCGGTAGAACCAGTCTcccc  >  2:185332/1‑249 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACC‑GTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGG‑AAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337151‑1337638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑