Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,767,014	C→T	G558S (GGT→AGT)	ydiJ ←	putative FAD‑linked oxidoreductase YdiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,767,014	0	C	T	96.4%	167.9 / NA	55	G558S (GGT→AGT)	ydiJ	putative FAD‑linked oxidoreductase YdiJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/33);  minor base G (0/2);  total (20/35)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCA  >  NC_000913/1766779‑1767256                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     ggCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTc                                                                                                                                                                                                                                       >  1:740078/1‑251 (MQ=255) ggCAGTTCTTGTTCCAGTTGGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGGTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTc                                                                                                                                                                                                                                       <  2:397722/251‑1 (MQ=255)     gttctttgtccatattgcgTGGAGCAACGCCGCGATCCGCCAACAAACGCAGCCATGCACGCACCAGCGTTGCGCGCCCTTCCGGGTAATGAATCCTGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCCCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACt                                                                                                                                                                                                                                   <  2:390015/233‑1 (MQ=255)      ttCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACCCATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGACTGTACTGCGCTTCACCGCGTCCACtt                                                                                                                                                                                                                                  >  2:462982/1‑251 (MQ=255)        cTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTtc                                                                                                                                                                                                                                >  2:186149/1‑251 (MQ=255)              ccAGTTTGAGGGGACCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCCCCAGCGTTGCCCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCatcatc                                                                                                                                                                                                                          <  2:704550/251‑1 (MQ=255)               cAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGCTCGAATGTACTGCGCTTCAACGCGTCCACTTTCATCATCg                                                                                                                                                                                                                         >  2:629446/1‑251 (MQ=255)                    tGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGTTCTGCCGATCGAATGTATTGCGCTTCACCGCGTCCACTTTCATCATCGgcgcc                                                                                                                                                                                                                    >  1:96762/1‑250 (MQ=255)                                cgccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCt                                                                                                                                                                                                        <  1:101582/251‑1 (MQ=255)                                cgccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCt                                                                                                                                                                                                        <  1:661481/251‑1 (MQ=255)                                 cccgcGATCCGCCAACAAACGCAGCCAATCACGCACCAGCGTTGCGCGCCCTTGCGGTGAATGAATCCGGTTCTGGGTGAGCTTTATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCTTCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCGCTTTCATCATCGGCGCATCGAGACCTTCTg                                                                                                                                                                                                       <  1:340400/250‑1 (MQ=255)                                                    cGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTgg                                                                                                                                                                                    <  1:629446/251‑1 (MQ=255)                                                    cGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTGACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTgg                                                                                                                                                                                    <  2:534471/251‑1 (MQ=255)                                                           aTTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGt                                                                                                                                                                             <  1:187815/251‑1 (MQ=255)                                                            ttCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCAATCGCACCGCGCCACTGCTGGCGTACCGCAATGGAGATCTGCCGATCGAATGTACTGCGCTTAACCGCGTCCACTTTCATCATCGGCGCATCGCGCCCTTCTGGCGGGCAACTCTTCCCTGGGTTGattc                                                                                                                                                                            >  2:394133/1‑248 (MQ=255)                                                               aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGt                                                                                                                                                                         >  1:722434/1‑251 (MQ=255)                                                               aCGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCGGGGTGACCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCGGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTAGGt                                                                                                                                                                         <  1:742919/251‑1 (MQ=255)                                                                   aCCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGGTATg                                                                                                                                                                     >  1:698041/1‑251 (MQ=255)                                                                    ccAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCCAATCTTCCCCGGGTTGAGTCGGCTAttg                                                                                                                                                                    >  2:253734/1‑249 (MQ=255)                                                                            gcgcgcCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCAACGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCACCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCaaa                                                                                                                                                            <  2:761097/251‑1 (MQ=255)                                                                                 cccTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCg                                                                                                                                                       <  2:128882/251‑1 (MQ=255)                                                                                     ttCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCt                                                                                                                                                     <  1:778470/249‑1 (MQ=255)                                                                                       cGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTc                                                                                                                                                  <  2:187338/250‑1 (MQ=255)                                                                                                 atCCGGGTCTGGGTGATCGTCATCGAAGGAACCATCGGACTACGGGCATCAAATTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAg                                                                                                                                       <  2:758836/251‑1 (MQ=255)                                                                                                           gggTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCaaaa                                                                                                                             <  1:462982/251‑1 (MQ=255)                                                                                                           gggTGATCTTCATCGACGGACACATCGGACTACGGGCATAAAAGTTGAAGCATAAACCTGTGCCGGTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTGTGCGCAGTTCTGCaa                                                                                                                               <  2:421819/249‑1 (MQ=255)                                                                                                                         gACGGAAACATCGGAAAAAGGGCATCAAAGTAGAAGCATAAACCGTTGAAGTTACAAGCCATCGAACCGCGCCACGGCTGGCGTATCGCAATGGGGAACTGCCGATCGAAGGTACTGCGCTTCACCGAGTCCACTTTCATCATCGGCGCAGCGAGACCTTCTGGAGGGCAAATCTGCCATGGGTGGAGACGGTTATGAGGGTCAAATGCAGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGaa                                                                                                               <  2:237003/251‑1 (MQ=255)                                                                                                                               cacaTCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTTAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGc                                                                                                         >  1:82932/1‑251 (MQ=255)                                                                                                                                 caTCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACGGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCg                                                                                                       <  1:351318/251‑1 (MQ=255)                                                                                                                                  aTCGGACTACGGGCATCAAAGTTGAAGCATAACCCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAACACGCCgg                                                                                                      >  1:594953/1‑251 (MQ=255)                                                                                                                                     ggACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCTTTTCACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCGCACCGAAAAACGCCGGGC‑t                                                                                                  <  2:74331/251‑1 (MQ=255)                                                                                                                                        cTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGt                                                                                                >  1:218373/1‑250 (MQ=255)                                                                                                                                             ggCATCAAAGTGGAAGCATAAACCGTGGCCGCTACACTCCATCGCACCGCGCCACAGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCTGCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATGCAg                                                                                          <  2:378518/251‑1 (MQ=255)                                                                                                                                               cATCAAAGTTGAAGCATAAACCGTGGCCGTTACACTCCAGCGCCCCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACGGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAgcg                                                                                        <  2:698041/251‑1 (MQ=255)                                                                                                                                                     aGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGaaa                                                                                   >  1:94190/1‑250 (MQ=255)                                                                                                                                                            gcaaaaaCCCTTGCCGTTACACACCATCGCACCGCGCCCCTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAAGGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTCCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGc                                                                           <  2:494950/247‑1 (MQ=255)                                                                                                                                                                aaaCCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTg                                                                       <  1:639488/251‑1 (MQ=255)                                                                                                                                                                       tGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCGTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTGCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGcc                                                                 <  1:801507/250‑1 (MQ=255)                                                                                                                                                                            ttACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCAGCTTTCACTTTGCGCAGTTCTGCAAAAAGATCCTCACCGCCAAACACCGGGC‑TGTATTCAGCGCGCAAAACTTTGCCggtcttgcaccaca                                                            >  1:798562/1‑237 (MQ=255)                                                                                                                                                                               caccccATCGCCCCGCGCCCCTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTGCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAc‑aacaa                                                       <  2:767057/247‑1 (MQ=255)                                                                                                                                                                                acTCCATCGCACCGCGCCACTGCTGGCGTACCGCAAGGGGGAACTGCCGATCGAAGGTACTGCGCTTCGCCGCGTCCACGTTCATCATCGGCGCAGCGAGACCGTCTGGCGGGCAAATCTACCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTGGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATGCAGCGCGAAAACCTTTGCCTTGCTCGCCCCAC‑AACaaa                                                      <  2:82505/251‑1 (MQ=255)                                                                                                                                                                                     aTCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAaccacc                                                 <  1:813570/251‑1 (MQ=255)                                                                                                                                                                                     aTCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAaccacc                                                 <  1:339393/251‑1 (MQ=255)                                                                                                                                                                                       cGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTTACTTTTCGCCGGTCTGCAAAAAGGTCCTCACCGAAAAACCCCGGGCCTG‑ATTCAGCGCCACAACCTTTGCCGTGCCCGCCCCACCAACAA‑CCACCgg                                               >  2:23626/1‑250 (MQ=255)                                                                                                                                                                                         cgccGCGCCACTGCCGGCGTACCGCAATGGTGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCAGCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTAt                                             <  2:122802/249‑1 (MQ=255)                                                                                                                                                                                          cccGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTAtt                                            <  1:418653/250‑1 (MQ=255)                                                                                                                                                                                            cgcgCTACTGCGTGCGTACCTCAATGGAGATCTGCCGATCGAATGTACTGCGCTTCTCCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCGGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCGCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCGTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTATTTc                                          <  2:544817/251‑1 (MQ=255)                                                                                                                                                                                              cgcCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTATTTCGc                                        >  1:181425/1‑251 (MQ=255)                                                                                                                                                                                                     gctgGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTATTTCGCAGTCa                                   <  1:600899/249‑1 (MQ=255)                                                                                                                                                                                                                       ggATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCagag               <  1:186149/251‑1 (MQ=255)                                                                                                                                                                                                                                gATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCCGTTTTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCCC‑CACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCCGAGATTTGCTtc      >  2:68385/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCCAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTACCGTGCTCGCCCCCC‑AAACAACCACCGTATTTCGCAGTCCGCGCCACCCCGTCATCAGAGATTTGCTtcat    >  2:204329/1‑251 (MQ=255)                                                                                                                                                                                                                                   cGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCTCCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTtcat    <  1:623600/250‑1 (MQ=255)                                                                                                                                                                                                                                    gAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGGAAACCTTTGCCGTGCTCGCCCCCC‑AACAAACCACCGTATTTCGCAGTCCGCGCCACCACGTCATCAGAGATTTGCTtcatt   >  1:690480/1‑249 (MQ=255)                                                                                                                                                                                                                                     aaTGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTtcatca  >  1:806107/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     GGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGC‑TGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCAC‑AACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCA  >  NC_000913/1766779‑1767256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑