Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,966,5300CT98.5% 226.7 / ‑3.9 66I38I (ATC→ATT) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (38/26);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01
*NC_0009133,966,5310CT98.5% 228.6 / ‑3.9 66L39L (CTG→TTG) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (39/26);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

ACTCTCCTGTGACCAGGCAGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TCCTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAA  >  NC_000913/3966292‑3966760
                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                        
cctctCCTGGGACCAGGCAGCGAAAAGACATGA‑GGCGATGACCTTAAACAGGCATGGATGACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCAc                                                                                                                                                                                                                              <  2:482811/250‑1 (MQ=255)
aCTCTCCTGTGACCAGGCAGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCAc                                                                                                                                                                                                                              >  1:244876/1‑251 (MQ=255)
 ctctCCTGTGACCAGGCAGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGCGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCC‑TTTTGAA‑GCAGCACg                                                                                                                                                                                                                             >  1:803912/1‑251 (MQ=255)
    cccttggACCAGCCAGCGAAAAGACA‑GACGGCGATGACCGTAAACAGGCATGTATTATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCaa                                                                                                                                                                                                                          <  2:607717/245‑1 (MQ=255)
          gACCAGGCAGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTg                                                                                                                                                                                                                    <  1:220885/251‑1 (MQ=255)
           aCCAGGCAGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAATgg                                                                                                                                                                                                                   >  2:17147/1‑251 (MQ=255)
             cAGGCAGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTCTGCGTAAGCAGGAAATTATTTTTTCCA‑TTTTGAA‑TCAGCACGCAAAGAGTGGCg                                                                                                                                                                                                                 >  2:727723/1‑251 (MQ=255)
             cAGGCAGCGAAAAGAAATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGACATACAATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATAAGCAGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGAGGCTGGCAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCAAGCAAAGAGTGGCg                                                                                                                                                                                                                 >  1:745993/1‑251 (MQ=255)
               ggCAGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGaa                                                                                                                                                                                                               <  1:510920/251‑1 (MQ=255)
                  aGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGa                                                                                                                                                                                                                >  1:587365/1‑247 (MQ=255)
                     gAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGat‑att                                                                                                                                                                                                        >  2:248616/1‑250 (MQ=255)
                        aaGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTTCCA‑TTTTGAA‑GCACCACGCAAAGAGGGGCGAAAAT‑ATCttt                                                                                                                                                                                                     >  2:430139/1‑251 (MQ=255)
                          gACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTgg                                                                                                                                                                                                   <  1:533515/251‑1 (MQ=255)
                                a‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAGCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATgg                                                                                                                                                                                             <  1:494606/251‑1 (MQ=255)
                                a‑GTCGATGACCGAAAACAGGCATGGATGATCCTGACATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGCAACTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGAAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGAAAGGAGTGGCGAAGAT‑ATATTTGGTGTTgg                                                                                                                                                                                             >  1:721115/1‑251 (MQ=255)
                                   tCGATGACCGTAAACAGGCATGGATGACCCTGCCATACCATTCACACCATTAAGTTCGAACTTTACCCCAAGTTTAAGAACTCACACCACTATAAATCTTAACCACTTAGAGACTCCGCCGGTTTCTCAACTGATCACTCTCGGCGGAAATATGGGGCTGGAAACCCTGGCTCGTATGCGTCAGCGGGACATTATTTTTGCCC‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGCAGaggtcgttaggtatggcg                                                                                                                                                                                            >  1:326960/1‑234 (MQ=255)
                                   gcGATGACCGTAAACAGGTATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCg                                                                                                                                                                                           <  2:492503/250‑1 (MQ=255)
                                    cGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGt                                                                                                                                                                                          >  1:669291/1‑251 (MQ=255)
                                            gTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGgaga                                                                                                                                                                                  <  1:402000/251‑1 (MQ=255)
                                             tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTgg                                                                                                                                                                                     >  1:745037/1‑247 (MQ=255)
                                              aaaCAGGCATGGATGAACCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAAGACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGata                                                                                                                                                                                <  2:62803/251‑1 (MQ=255)
                                                    gaagtgatgctCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTCCTGTAGATATTGCAg                                                                                                                                                                          <  2:169426/246‑1 (MQ=255)
                                                           tgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAggatggat                                                                                                                                                                   >  1:574178/1‑251 (MQ=255)
                                                             atCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAGGAT‑ATCTTTGGTGTTGGGGTACTGGGGATATTGAAGGATGGAttt                                                                                                                                                                 >  2:715332/1‑251 (MQ=255)
                                                             atCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGAttt                                                                                                                                                                 <  1:550934/251‑1 (MQ=255)
                                                                  gccattacATTCTCAACATTAAGTTCGAGATTGACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCATGATTGATTTGGtt                                                                                                                                                             <  2:734444/243‑1 (MQ=255)
                                                                     aTACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGAAGGATGGATTTGGTTtcct                                                                                                                                                         >  2:638072/1‑251 (MQ=255)
                                                                          aTTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTg                                                                                                                                                                <  1:428566/239‑1 (MQ=255)
                                                                                aacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGc                                                                                                                                                >  1:114919/1‑249 (MQ=255)
                                                                                        gTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTCCTGGAGATATTGCAGGATGGATTTGGTTTCCTCAGTTCCGCAgcacgatcc                                                                                                                                      >  2:114516/1‑243 (MQ=255)
                                                                                          tCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCtc                                                                                                                                    >  2:196404/1‑250 (MQ=255)
                                                                                           cGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCGTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCGCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTa                                                                                                                                    <  1:722673/250‑1 (MQ=255)
                                                                                               aTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCg                                                                                                                               <  1:17147/251‑1 (MQ=255)
                                                                                               aTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAGCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCg                                                                                                                               <  1:8018/251‑1 (MQ=255)
                                                                                                  tACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGcc                                                                                                                             <  1:347074/250‑1 (MQ=255)
                                                                                                    ccccAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGCGATATTGCCGGATGGATTTg                                                                                                                                                                >  1:43321/1‑213 (MQ=255)
                                                                                                    ccccAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTg                                                                                                                                                                <  2:43321/213‑1 (MQ=255)
                                                                                                         aGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGGTTTGGTTTCCTCCGTTCCGCAGACAGCTCCCACCTCCCCGGTCCtgt                                                                                                                     >  2:671741/1‑250 (MQ=255)
                                                                                                         aGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCtg                                                                                                                      >  1:791141/1‑250 (MQ=255)
                                                                                                            ttAAGAACTCACACCACTATGACTCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGGTGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCtgatgc                                                                                                                  >  2:215532/1‑250 (MQ=255)
                                                                                                            ttAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCCCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑ATTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATATTTGGTGACGGCGTAATGGAGATATTGCAGGATGGATTTGGTTTCTCCCGTTCCGCAGTAAGATCCTACCTCGCCGGTCCtgatg                                                                                                                   >  2:542529/1‑250 (MQ=255)
                                                                                                              aaGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGAc                                                                                                                 <  2:194724/250‑1 (MQ=255)
                                                                                                                  aCTCAAACCACTATGAAACTTACCGAATTAACGACTACGCCGGTTTCTGCGCAGATCACTCTCAGCGAAAATATGGAGCTGGAAAACCTGGCTCGAATGCGTACGCAGGAAATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGCTGGCGCACTGGGGATATTGAAGGTAGGCTTTGGTTTCCTCGGTTACGCAGACCGCCCCTACCTCCCCgatcggaatgtcatct                                                                                                             >  2:537519/1‑235 (MQ=255)
                                                                                                                   cTCACACAACTATGAATCTTACCGAATTACAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCCCGCAAAAAGTGGCGAAGCT‑ATCTTTGGATAAGGCGGACTGGAGATATTGACGGCAGGATTTGGTTTCCTCCGTTCCGCAGACCGCTCCTACCCAGCCGGTCCtgattgcatcctc                                                                                                           >  2:88630/1‑242 (MQ=255)
                                                                                                                          cacTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACCCTCGGCGAAAATATGGGGCTGGCAAACCTGGCTCGAATGCGTAAGCAGGACATTATTTTT‑CCACTTTTGAA‑GCAGCCCGCGAAGAGGGGCGAAGAT‑ATCTTTGGGGATGGAGTACTGGAGATATTGGAGGATGGGTTTGGTTTCCTCCGTGCCGCAGACAGCTCCTACCTCGCCGGTCCTTGTGGCATCTACGTTAccc                                                                                                    >  1:62859/1‑251 (MQ=255)
                                                                                                                           acTATGAAGCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAATCCTGGCTCGTATGCGTAAGCAGGACATGATTTGTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATGTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGTTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTcccc                                                                                                   <  1:112660/251‑1 (MQ=255)
                                                                                                                                   tCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATgg                                                                                                                                                                                             >  2:196766/1‑153 (MQ=255)
                                                                                                                                   tCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATgg                                                                                                                                                                                             <  1:196766/153‑1 (MQ=255)
                                                                                                                                          gAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGTATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAACCAAATccgccg                                                                                    >  1:474163/1‑251 (MQ=255)
                                                                                                                                           aaTTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGgag                                                                                                                                                                                   <  1:179905/155‑1 (MQ=255)
                                                                                                                                           aaTTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGgag                                                                                                                                                                                   >  2:179905/1‑155 (MQ=255)
                                                                                                                                           aaTTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGt                                                                                   >  1:436711/1‑251 (MQ=255)
                                                                                                                                                  gAATACGCCGGTTTCTGAGCTGCTCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCCGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGCCAGCTCCTACCTCGCCGGTCCTGATGCCCTCTACGTTTCCCCTAGCCAAATCCGCCGttttcacc                                                                            >  2:327458/1‑246 (MQ=255)
                                                                                                                                                  gAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTACGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAAcc                                                                            >  2:314137/1‑251 (MQ=255)
                                                                                                                                                           ggTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCATGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTg                                                                   >  1:22386/1‑251 (MQ=255)
                                                                                                                                                           ggTTTCTGAGCGGATCACTCTCGGCGAAAATATGGGGCGGGAAACCCTGGCTCGTAGGCGTAAGCAGGACATTATTTTTGCCA‑TTTGGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑AGCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCATACAGCTCTTCCCTCGCCGGTCCTTAGGACAGCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTg                                                                   <  1:715332/251‑1 (MQ=255)
                                                                                                                                                                                    gAAAATATGGGGCTGGAAACCCTGGCTCCTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGAGGCCAAGAT‑ATCTTTGGTTATGGCGTACTGGAGATATTGCCGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCCAATCCGCCGTTTCAACCTCCGCCCTGGTGATACC‑CTCTCTGGCAAGAtttc                                         >  2:416004/1‑249 (MQ=255)
                                                                                                                                                                                                     aacccTGGCTGGTAGGCGTAAGCGGGACATTATTTTTGC‑T‑TTTTGAAAGCAGCAGGCAAAGAGGGGCGAAGAT‑TTCTTGGGTGAGGGCGTACGGGAGATATTGAGGGATGTATTTGGTTCCCTCCGTCCCGCAGACAGCTCCTCCCTCGCCGTTCCTTAGGACATTTACGTTCCCCCTAGCAAAATCCGCCGTTTCAACCCCCGAACGGGTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTg                        <  1:114516/248‑1 (MQ=255)
                                                                                                                                                                                                         cTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCCGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGCACg                    >  2:13831/1‑251 (MQ=255)
                                                                                                                                                                                                          tGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGc                   >  2:599632/1‑251 (MQ=255)
                                                                                                                                                                                                            gctggttggCGTAAGCAGGATATTAGTTTTGCCT‑TTTCGAA‑TCAGCACGCAAAGAGTGGC‑AATATTATCTTGGGTGATGGCTGGCTGAATATATTGCAGGATGGATTGGTTTTCCTCCGTTCCGCAGACAGCTCTTACCCCGCCGGTCCTGATGACACCTACTGTTCCCTTAGCCAAACCCGCCGTTTCACCCCCCGCACTTTTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGAACGTGAACGCTa                 <  1:676039/243‑1 (MQ=255)
                                                                                                                                                                                                            gCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGCCAGCTCCTACCTCGCCGGTCCTGCTGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGACGGGGAACGCt                  >  2:470783/1‑250 (MQ=255)
                                                                                                                                                                                                               cttATGCGAAAGCGGGACATTTTTTTTCCCT‑TTTTAAA‑GCGGCACGAAAAGAGGGGGGAAGAT‑ATTTTTGGTGATGGCGTACGGGAGATATGGCAGGAGGGATTTGGTTTCCCCCTTCCCGCAGACAGCCCCTCCCCCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCCCCGCACTGGTGATCCC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAt                <  1:248616/247‑1 (MQ=255)
                                                                                                                                                                                                                  aTGCGTAAGCAGGACATTATTTTTGCCA‑TCCTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTCCCTCGCCGGTCCTGATGGCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGGAAGAAGGGTAACGCTAtttttc           >  2:176891/1‑249 (MQ=255)
                                                                                                                                                                                                                         aGCAGGACATTATTTTTGCCA‑TATTGAA‑GCAGCACGCAAAGAGTGGCGAGGAT‑ATCTTTGGGGATGGCGTACTGGAGATAGTGCTGGGTGGATTTGGTTTCCTCCGTTCCGCAGACAACTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCCAATCCGCCGTTTCAACCTCCGCACTGG‑GGTACCCATCTCTGGAAACATTCGACAGCCGAAAGAAAGTGAACGCTAtttttctcatacg    >  2:591576/1‑242 (MQ=255)
                                                                                                                                                                                                                             ggACATTATTTTTGCCA‑TTTTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaa  >  1:729097/1‑249 (MQ=255)
                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                        
ACTCTCCTGTGACCAGGCAGCGAAAAGACATGA‑GTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCA‑TCCTGAA‑GCAGCACGCAAAGAGTGGCGAAGAT‑ATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACC‑ATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAA  >  NC_000913/3966292‑3966760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑