BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,217,310	A→G	S68G (AGC→GGC)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,310	0	A	G	92.5%	317.0 / 14.7	107	S68G (AGC→GGC)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand): ref base A (3/5); new base G (51/48); total (54/53)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-02

Only 100 of 107 total aligned reads displayed.

CCGCCTGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCA‑TTGAGCCGGGCGATCCGCGCTATGTCGATT  >  NC_000913/4217064‑4217556

ccGCCTGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCATATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCt                                                                                                                                                                                                                                                         >  1:318399/1‑251 (MQ=255)

    cTGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCt                                                                                                                                                                                                                                                         >  1:472726/1‑247 (MQ=255)

    cTGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCt                                                                                                                                                                                                                                                         <  2:472726/247‑1 (MQ=255)

     tGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAGGGCTACATCAACGGCCTCGgcg                                                                                                                                                                                                                                                    >  2:504502/1‑251 (MQ=255)

       gtAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAACTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCT‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCa                                                                                                                                                                                                                                                  <  2:136563/250‑1 (MQ=255)

        tAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCac                                                                                                                                                                                                                                                 >  1:536981/1‑251 (MQ=255)

        tAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCac                                                                                                                                                                                                                                                 >  1:132418/1‑251 (MQ=255)

          gtgTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCactg                                                                                                                                                                                                                                               <  2:52978/249‑1 (MQ=255)

          gCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCactg                                                                                                                                                                                                                                               <  2:250626/251‑1 (MQ=255)

              aaaCCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCactgactg                                                                                                                                                                                                                                           >  2:347595/1‑251 (MQ=255)

               aaCCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTgg                                                                                                                                                                                                                                          >  1:556087/1‑251 (MQ=255)

                accaccACATCCCTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGCGGTGAAATTACGAGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAACTGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACCTCAACGGCCTCGGCGCACTGACTGGc                                                                                                                                                                                                                                         >  2:805340/1‑251 (MQ=255)

                   cccacATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGt                                                                                                                                                                                                                                      <  1:11974/250‑1 (MQ=255)

                   accacATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGt                                                                                                                                                                                                                                      <  1:620276/251‑1 (MQ=255)

                      acaTAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAg                                                                                                                                                                                                                                   <  2:652372/251‑1 (MQ=255)

                             tATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGGCAGgcgcggg                                                                                                                                                                                                                            >  2:147214/1‑248 (MQ=255)

                              aTGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCATTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGAGAGTCGAAAAACGGCTACATCAACGGCCTCGGCGCACTGACTGACGGTCAggaggtgc                                                                                                                                                                                                                            >  1:533253/1‑244 (MQ=255)

                              aTGGAGCATCTGCACATGAAAACCCGTACACAACAAAGTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCa                                                                                                                                                                                                                           <  1:423557/251‑1 (MQ=255)

                                ggAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGGGCTGCaa                                                                                                                                                                                                                          >  1:565849/1‑250 (MQ=255)

                                ggAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAAGGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAAc                                                                                                                                                                                                                         <  2:699690/251‑1 (MQ=255)

                                 gAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAAc                                                                                                                                                                                                                         >  1:683267/1‑250 (MQ=255)

                                 gAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACa                                                                                                                                                                                                                        <  1:147214/251‑1 (MQ=255)

                                    cATCTGCACATGAAAACCCGTACACACCAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGc                                                                                                                                                                                                                     >  1:715930/1‑251 (MQ=255)

                                     aTCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCg                                                                                                                                                                                                                    >  1:300523/1‑251 (MQ=255)

                                         gCACATGAAAACCCGTACACAACAAAATGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCGTACAGTGCGGCAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAATTGTGGCGTCTGCTGAACGGTGAGGCGAAAAAAGGATACATCAACGGACTCGGCGCCCTGCATGGCGGTAAGGCGCTGccaaagaacga                                                                                                                                                                                                                  >  2:216661/1‑239 (MQ=255)

                                             aTGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAACAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTTAgggcctgcgacagaggaaatgggg                                                                                                                                                                                                            >  2:29930/1‑229 (MQ=255)

                                                       gTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TAATGAAGc                                                                                                                                                                                                  >  1:378252/1‑250 (MQ=255)

                                                            caacaaATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCAATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCCCTGCCTGGCGGTCAGGCGCGGCAACAGGCGCAAGCGGG‑GGTTGGAGCAGTCtt                                                                                                                                                                                            >  2:678855/1‑250 (MQ=255)

                                                                aaagtGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTTGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCTGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTg                                                                                                                                                                                        <  2:442011/247‑1 (MQ=255)

                                                                    tGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCgg                                                                                                                                                                                    >  1:590977/1‑251 (MQ=255)

                                                                      aagaaTTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCgggg                                                                                                                                                                                  >  1:656377/1‑250 (MQ=255)

                                                                      aagaaTTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGGGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGAGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCggg                                                                                                                                                                                   >  1:151962/1‑250 (MQ=255)

                                                                         aaTTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCCGGCGCTGCAACAGGCGAAAGCGGG‑GATTGAAGAAGTCTATCTTTCggggggg                                                                                                                                                                               >  2:189653/1‑247 (MQ=255)

                                                                           ttACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CCGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGACAGGGGCTGGAACAGGCGAAAGCGGGCTATT‑AAGGCGTCTATCTAGCGGGATgg                                                                                                                                                                               >  1:409409/1‑249 (MQ=255)

                                                                           ttACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCCAGCGCCGCACCAGGCGGAAGCGGG‑TATTGAAGCAATCTCTCTGTCGGGATGGcc                                                                                                                                                                             >  2:790482/1‑250 (MQ=255)

                                                                             aCAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAACAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑GATTGAAGCAGTCTATCTTTCGGGATGGCAgg                                                                                                                                                                           >  1:95505/1‑251 (MQ=255)

                                                                               aGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGTAGATGTGGTGAAATTACGAGGTTCAGTCAATCCTGACTGCACGCTGGCGCAACTGGGCG‑CAGCGAACATGAGGCGTCTGCTGCACGGTGAGTCGAACAAAGGCTACATCAACGGCCTCGGCGCACTGCCTGGCGGTCAGGCGCTGCCACCGGCGCAAGCCGG‑TATTTAAGGCGTCTATCTGGCGGGGTGGGAgggg                                                                                                                                                                         >  1:750264/1‑249 (MQ=255)

                                                                                 aaaGAGAGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGCTGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCCACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAAAAGGCGAAAGAGGG‑GATTGAAGCAGTCTATCTGTCGGGAtcgccagtagc                                                                                                                                                                       >  1:813155/1‑241 (MQ=255)

                                                                                    gagTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAgcggc                                                                                                                                                                    <  1:27854/251‑1 (MQ=255)

                                                                                    gagTGGACGCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAgcggc                                                                                                                                                                    <  1:482246/251‑1 (MQ=255)

                                                                                     aggggACGCAACCGCGTTGGGAAGGCATTCCTCGCCCATACAGGGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CATCGAAAAGGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAgcggcg                                                                                                                                                                   <  1:814207/248‑1 (MQ=255)

                                                                                      gTGGACTCAACCGCGTTGGGAAGGCATTACGCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGcggcgg                                                                                                                                                                  <  1:60780/251‑1 (MQ=255)

                                                                                        ggACTCAACCGGGGTGGGAAGGCATGACGCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAAGCCTGAAGGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGGGGCGTCTGCTGCACGGTGAGGCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGAc                                                                                                                                                                <  2:177926/251‑1 (MQ=255)

                                                                                        ggACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTTAAGCAGTCTATCTGTCGGGATGGCAGGTAGcggcgggc                                                                                                                                                                >  1:709869/1‑249 (MQ=255)

                                                                                          aCTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCCACAGGCGCAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGGAGCGGCGGACGc                                                                                                                                                              >  1:100910/1‑251 (MQ=255)

                                                                                               aCCGCGTTGGGAAGGCATTACGCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAAGCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGGCGGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TAGTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AAcc                                                                                                                                                        <  2:710428/251‑1 (MQ=255)

                                                                                                      tGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAAGTACGCGGTTCAGTCAAGCCTGAATGCACGCTGGCGCTACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGTG‑TATTGAAGCAGTCTATCGGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTggcgg                                                                                                                                                  <  2:127364/250‑1 (MQ=255)

                                                                                                           aGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGTAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAACAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCCGGGGCTGCAACAGGCGGACGCGGG‑TATTGAAGACGGCTATCTGTATGGAAGGCAGGTAGCGGCGGaggacgccctcgcggccagcc                                                                                                                                             >  2:270967/1‑231 (MQ=255)

                                                                                                             gCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGGGCTGCAACAGGCGAAAGCGGG‑GATTGTAGCCGTCTATCTGTAGGGATGGCCGGTAGCGGCGGGCGCC‑AACATGGCGGCCAGCATg                                                                                                                                          >  1:373284/1‑251 (MQ=255)

                                                                                                              cATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGAGAAAATGTGGCGTCTGCTGCACGTTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGt                                                                                                                                         <  1:718460/251‑1 (MQ=255)

                                                                                                              cATTACTCGCCCATACAGTGCGGAAGATGTAGTGAAATTACGCGGTTCAGGCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGAGGAAATGTGGCGTCTGCTGCACGGGGAGTAGAAAAAAGGCTACATCAAGGGCCTCGGCGCACTGACTGGCGGGCAGGCGCTGCAACCGGCGGAAGGGGG‑TATTGTAGAAGACGATGTGTCGGGATGGCGGGTAGCGGCGGaggccagccttgggggcagcaggt                                                                                                                                          >  1:541304/1‑228 (MQ=255)

                                                                                                                  aCTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATcc                                                                                                                                     <  1:678855/251‑1 (MQ=255)

                                                                                                                  aCTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑GATTGAAGCAGTCCATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCCGGCGGCCAGCATGTATcc                                                                                                                                     >  1:596497/1‑251 (MQ=255)

                                                                                                                   cTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑GATTGAAGAAGGCTATCTGTCGGGATGGCCGGGAGCGGCGGAAGCC‑AACCTGGAGGCCAGCAAGGATCCg                                                                                                                                    >  1:610591/1‑251 (MQ=255)

                                                                                                                    tCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCCGGCGGCCAGCATGTATCCgg                                                                                                                                   >  1:415441/1‑251 (MQ=255)

                                                                                                                         cATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCCGGCGGCCAGCAAGTATCCGGATc                                                                                                                                >  1:347516/1‑249 (MQ=255)

                                                                                                                            aCAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAAGGTGGCGTCTGCTGCACGGTGAGGCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTc                                                                                                                            <  2:460238/250‑1 (MQ=255)

                                                                                                                              aGTGCGGAAGAGGTGGTGAAAGTACGCAGTTCAGTCAAGCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCGCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGct                                                                                                                         <  2:565849/251‑1 (MQ=255)

                                                                                                                                   ggAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAAACGGGGGAAGCGGG‑GATTGAAGCAGTCTATCTGTCGGGATGGGAGGGAGCGGCGGACGGT‑AAACAGGGGGCCAACGTGTATcctgggcacgcgcgctttc                                                                                                                    >  2:748548/1‑234 (MQ=255)

                                                                                                                                        aTGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCa                                                                                                               <  2:394113/251‑1 (MQ=255)

                                                                                                                                          gtggtgAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGAGAAAATGTGGCGTCTGCTGCACGGTGAGTCGACAAAAGGCTACATCCACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCACCAGGCGAAAGCGGG‑GATTGAAGCAGTCTATCTGTCGGGGTGGCAGGTAGCGGCGGAAGAT‑AACCTGGCGGGCCGCAATTATTCGGATCCATAGCTCTATCCGGCaa                                                                                                              >  1:525113/1‑250 (MQ=255)

                                                                                                                                                  atgccgcgGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTTGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGAGCAGTCGCTCTATCCGGCAAACTC‑GGTGc                                                                                                    <  2:62970/247‑1 (MQ=255)

                                                                                                                                                          gTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCtgtg                                                                                            <  1:100004/251‑1 (MQ=255)

                                                                                                                                                             cAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAGGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTgtggtg                                                                                         <  1:504502/251‑1 (MQ=255)

                                                                                                                                                             cAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGGCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTgtggtg                                                                                         <  1:805340/251‑1 (MQ=255)

                                                                                                                                                                tCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGACGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑GATTGAAGCAAGCTATCTGTCGGGTGGGCAGGGAACGGCGGACGCC‑CACCTGGCGGCCAGCATGTAACCAGGTAAGACGACCTATCCGGCAAACTC‑TGGGCCAGCtcgggggg                                                                                        >  1:325363/1‑242 (MQ=255)

                                                                                                                                                                 caaaccTGAATGCACGCGGGCGGAACTGGGCG‑CAGCTAAAATGTGGCGTCTGCGGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACGGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAg                                                                                      <  2:816513/246‑1 (MQ=255)

                                                                                                                                                                  aaTCCTGAATGCACGCTGGCGCAACTGGGCG‑CAACGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGAACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGAAAACTC‑GGTGCCAGCTGTGGTGGAG‑Cg                                                                                   <  1:115977/251‑1 (MQ=255)

                                                                                                                                                                   aTCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCCGGTAGCGGCGGACGCT‑AACCTGGCGGCCCGCATGTATCCGGGTCAGGCGCTCTCTTCGGCAAACTC‑GGGGCCAGCTGTGGTGGAG‑Cgg                                                                                  >  1:277715/1‑251 (MQ=255)

                                                                                                                                                                   aTCCTGAATGCACGCGGGCGCAA‑TGGGGGTCAGCGAAAATGTGGCGTCTGCGGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACCC‑GGTGCCAGCTGTGTTGGAG‑Cgg                                                                                  <  2:135213/251‑1 (MQ=255)

                                                                                                                                                                     ccggaatgacagctgggggaacggggCG‑CATCGAAAATGGGGCGGCTGCTGCACGGGGAGTCGAAAAAAGGCGACAGCAACGGCGTCGGCGCACTGACGGGCGGTCAGGCGCGGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCGATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGAGCAGGCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑Cggct                                                                                <  2:727544/228‑3 (MQ=255)

                                                                                                                                                                        gaatgccatgtggggcAACTGGGCG‑CGGCGAAAATGTGGCGTTGGCTGCTCGGTGAGTCGAAAAAAGGCTACATCAACGGCCGCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTTAAGCAGTCTAGCTGTCTGGATGGCAGGTAGCGGCGGTCTCG‑TACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGAGcaa                                                                             <  2:750264/237‑1 (MQ=255)

                                                                                                                                                                        gAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGA‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATcaa                                                                             <  1:721566/251‑1 (MQ=255)

                                                                                                                                                                        gAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCGGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATca                                                                              <  2:709869/250‑1 (MQ=255)

                                                                                                                                                                          aTGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCCTGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATcaac                                                                            >  1:197748/1‑250 (MQ=255)

                                                                                                                                                                          aTGCACGCTGGCGCAACTGGGCG‑CAGCGAAAAGGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATcaaca                                                                           <  2:415441/251‑1 (MQ=255)

                                                                                                                                                                            gCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCaacaac                                                                         >  1:376159/1‑251 (MQ=255)

                                                                                                                                                                            gCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAATCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCaacaac                                                                         <  1:83101/251‑1 (MQ=255)

                                                                                                                                                                              aCGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCCACAGGCGAAAGCGGG‑GATTGGAACCGTCTATCTGTCGGGGTTGCAGGGAGCGGCGGGAGCG‑CACCCTACGGCCCGCATGTATCCGGATCACACGCTCTATCCGGCCAACCC‑TGTGCCCCCTGGGGTGGAggaggtcaaccaccc                                                                        >  2:481227/1‑237 (MQ=255)

                                                                                                                                                                                   ggCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACGGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGTATCAACAACACCTTc                                                                   <  2:151962/250‑1 (MQ=255)

                                                                                                                                                                                      gcAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCcgtc                                                               <  1:472288/251‑1 (MQ=255)

                                                                                                                                                                                       cAACTGGGCG‑CTGCGAAAATGTGGCGTTGGCTGCACGGTGAGTCGAAAAAGGGCGACATCAACGGCCTCGGCGCACTGACTGGCTGTCAGGCGCGGCAACAGGCGAAAGCGGG‑TATTTAAGCAGTCTAGCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑ATCCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGTCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACTCCTTCcgtcg                                                              <  2:430311/251‑1 (MQ=255)

                                                                                                                                                                                        aaCTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑GATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCC‑AACCTGGCGGGCAGCATGTATCCGGGTCAGGCGGTCTATCCGGCAAACTC‑GGTGCCAGCTGGGGCGGGG‑CGGGGTAACAACAACTTCcggcgg                                                             >  1:361866/1‑247 (MQ=255)

                                                                                                                                                                                         aCTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCCGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTg                                                            >  1:523256/1‑251 (MQ=255)

                                                                                                                                                                                            gggCG‑CAGCGAAAATGTGGCTTTTGCTGCACTGTGAGTCGAAAAAAGGCTACATCAACGGCCGCGGCGCACTGACTGGCGGTCAGGCGTTGCAACAGGCGAAAGCGGT‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCGGGCGGCCAGCATGTAGCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTg                                                            <  2:50527/248‑1 (MQ=255)

                                                                                                                                                                                            gggCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCCGGCGGCCAGCATGTATCCGGATCAGTCGCCCTATCCGGCAAACTC‑GGTGCCCGCTGTGGGTGAG‑CGGATCAACAACACCTTCcctcggggcg                                                         >  1:258562/1‑246 (MQ=255)

                                                                                                                                                                                                      gAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATcc                                                <  1:347595/251‑1 (MQ=255)

                                                                                                                                                                                                       aaaaTGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCa                                               <  2:247282/251‑1 (MQ=255)

                                                                                                                                                                                                       aaaaTGGGGCGTCTGCTGCACGGGGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCa                                               <  2:100910/251‑1 (MQ=255)

                                                                                                                                                                                                          atggggCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATg                                            <  2:318399/247‑1 (MQ=255)

                                                                                                                                                                                                            gtgGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGt                                          <  1:189653/251‑1 (MQ=255)

                                                                                                                                                                                                                cGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGGGCTGCAACAGGCGAAAGCGGG‑GATTGAAGCAGTCTATCTGTCGGGGTGGCAGGGTGCGGCGGGAGCT‑AACCTGGCGGCCCGAGAGTATCCCGATCAGGCGCTCTTTCCCGCCAACTC‑AGTGCCAGCCGTGGGGGAG‑GGGATCCACAACAACCTCcgtccggccgggcccatccacaagtccgc                                      >  2:110704/1‑226 (MQ=255)

                                                                                                                                                                                                                     gtggcACGGGGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGc                                  <  2:707666/247‑1 (MQ=255)

                                                                                                                                                                                                                             gTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGAGGG‑GATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGGCGCT‑AACCTGGCGGCCAGCATGTATCCGGGTCAGGCGCTCTTTCCCGCAAACTC‑GGTGCCAGCCGTGGGGGGG‑CGGGTCAACAACACCTTCCGGCGGGCCGACCAGATCCAATGGTACCCGGGCC‑TTGAGCCg                        >  1:803978/1‑251 (MQ=255)

                                                                                                                                                                                                                                         aaGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCCACAGGCGAAAGCGGG‑GATTGAAGCAGTCTATCTGTAGGGGTGGCAGGGAGCGGCGGGCGCTTAACCTG‑CCGCCAGCCAGTATCCCGGCCAGTCGCCCTATCCAGCAAAATCCGGTGCC‑GCCGTGGGGGGGGCGGTTCAACA‑CCCCTTTCCTCGTTCCGATCAGATCCAAATGTTCTCGGGCA‑TTGAGCCAGGCGagcacacg            >  2:494580/1‑244 (MQ=255)

                                                                                                                                                                                                                                          aGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCTAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATTGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCTGATCAGTCGCTCTATCCGGCAAATTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCA‑TTGAGCCGGGCGATCCGCGCt           <  1:797733/251‑1 (MQ=255)

                                                                                                                                                                                                                                            gCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAAAAGGCGAAAGCGGG‑GAGTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGAAGCT‑AACCTGGCGGCCCGGAGGTATCCGGATAAGTCGCTCTATCCGGCACACTC‑GGGGCCAGCTGTGGTGGAG‑CGGATCAACACAAACTTCCGTCGTTCCGGTCAGATCCAATGGACCGCGGGCAATTG‑GCCGGGCGATcccccacat         >  1:131831/1‑244 (MQ=255)

                                                                                                                                                                                                                                                cATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCCACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCCTGTATCCGGGTCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCCTGCCGAACAGGTCCAATGGACCGCGGGCA‑TTTAGCCGGGCGATCCGCGCTATggcg     >  1:258048/1‑248 (MQ=255)

                                                                                                                                                                                                                                                   cAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCCGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCC‑TTGAGCCGGGCGATCCGCGCTATGTCGAtt  >  1:803940/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   cAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCA‑TTGAGCCGGGCGATCCGCGCTATGTCGAtt  <  1:816866/251‑1 (MQ=255)

CCGCCTGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCG‑CAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGG‑TATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTC‑GGTGCCAGCTGTGGTGGAG‑CGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCA‑TTGAGCCGGGCGATCCGCGCTATGTCGATT  >  NC_000913/4217064‑4217556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`