Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	112.3 / NA	36	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

GTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGC  >  NC_000913/1652094‑1652570                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  gtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATgg                                                                                                                                                                                                                                      <  2:556164/251‑1 (MQ=255) gtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATg                                                                                                                                                                                                                                       <  1:310641/250‑1 (MQ=255)                ggAGGTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAG‑GACATAGAACTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGaaaaa                                                                                                                                                                                                                        <  2:243654/249‑1 (MQ=255)                   gTTCGCCCTGGTGAGGTGTGTGCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAAAGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTATCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACaaaa                                                                                                                                                                                                                   <  2:97437/251‑1 (MQ=255)                   gTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGAATGGCAGAAA‑AAGAAAACaaa                                                                                                                                                                                                                    >  1:108636/1‑250 (MQ=255)                       gCCCTGGTGAGCTGTGTGCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGCGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTTTTTAACCAGCAATCGCCGCTCTCC‑GGCGTCAGATGGAATTTACATGACTGAGCAAAGGGCATATCATTGATTTTCACCTCCGAGGGTATGGCCGAAA‑AAAAAAAAAAAAAtac                                                                                                                                                                                                               >  2:761594/1‑249 (MQ=255)                               gAGCTGTGTGCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAAACGACGACCTCC‑GGAATCAAGTGACATTAACAAGACTGAGAAAAAAGCATATCATTTAAGTTCACCCCACGGAGTATTGCCGAACCAGAAAAA‑AAAAATGCAC‑CTTTGt                                                                                                                                                                                                      >  1:516259/1‑251 (MQ=255)                                    gtgtgCACTTGGATGGGAG‑GACATAGAACTGAAAAATGGAACAATGATGAGCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTAGTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACCGAGCAAATATCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTc                                                                                                                                                                                                 <  2:537503/251‑1 (MQ=255)                                     tgtgCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGTCATTCATCTTATTAAGACACCAATCGACGCTCTCCCGGAG‑CAGATGACATTAACGAGAATGAGCAAAAAGCATATAATTGATGTTTACCTCCGAGAGGATGGCAAAAC‑AGAAAAACAAAAAAGCAC‑CTTTTTTTTTcc                                                                                                                                                                                                >  2:142437/1‑250 (MQ=255)                                      gtgCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCa                                                                                                                                                                                                <  2:20930/250‑1 (MQ=255)                                         cACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAAcc                                                                                                                                                                                             <  2:307509/250‑1 (MQ=255)                                             tGGATGGGAGTGA‑ATAGAACTGAAAAATGGAACAAAGATGATCAGGAGAAATTTATCAAAAGACATTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTAGTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCCC‑CTTTGTTTTTCAACCTgta                                                                                                                                                                                         <  2:772466/250‑3 (MQ=255)                                               gATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGGCATTCATCTTATTAAGACAGCAATCGACGCTCTCC‑CGAGTCAGATGACATTAAAGAGACTGGGCAAAAAGCATATAATTGTTGTTCACCTCAGAGAGTATGGCACAAC‑AACAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTCAAgtg                                                                                                                                                                                      >  1:758144/1‑251 (MQ=255)                                                   ggag‑gaCATAGATCTGACAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGAGGGAACCAAACGGGTCATTCCTCTTATTAAGCCAGCAAACGCCGCTCTAC‑GGAGTCAGATAACATAAACGCGACTGAGCAAAAAAAATCTCATTAATTTTCACCTCAAAGAGTAAGTAAACAA‑AAAAAAACAAAAATTCAC‑CCTTTTTTTTCaaacctaagagta                                                                                                                                                                                    >  2:780119/1‑238 (MQ=255)                                                               aTCTGAAAAATGGAACAATGATGATTAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAACCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGTaaa                                                                                                                                                                        >  1:585294/1‑250 (MQ=255)                                                                   gAAAAATGGAACAAATATGACCATGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTGGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaaaa                                                                                                                                                                     <  2:266920/249‑1 (MQ=255)                                                                                 tgatgaTCAGGAGAAATGTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGTAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagag                                                                                                                                                                                                                                             <  1:581631/165‑1 (MQ=255)                                                                                   atgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGACACAAACGGGACATTCATCTTATTAAGACAGCAATCGAAGCCCTCC‑GGGGTAAGATGACATTAACGAGACTGGGCAAAGACGATATCATTTGTGGTCACCTCCGCGAATATAGCCGAAC‑AAGAAAACACAAATG‑ACACTTTTTTTTTCAAACATAAGAGTCAGCGAGAGAAAAAAATTATTGTGACCATTTTAc                                                                                                                                                   >  1:201064/1‑251 (MQ=255)                                                                                   atgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTAc                                                                                                                                                   <  1:447309/251‑1 (MQ=255)                                                                                                               gTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTGATTCATCTTATTAAACCCACAATAGACGCTCTCC‑GGAGTCAGAAGACATTAACAAGAATGAGCACAAAACAAATACTTGCAGTTTACCTCAAAGAGTATGGCAGAAC‑AGAAAAACAAAAATGAAC‑CTTTGTTTTTCAACCTGAAGAGTCAACCAAAGGAAAAAATTATTGTGACCCTTTTTCCGTTTACTCAATAAGACAGaataggggg                                                                                                                       >  2:548221/1‑243 (MQ=255)                                                                                                                 ttCACGGTACCAAAAACACAGGCGGGAACCCATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTTAACCTGAAGAGTCAGCGAGAGAAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGc                                                                                                                     >  1:808996/1‑251 (MQ=255)                                                                                                                 ttCACGGTACCAAAAACACAGGCGAGGACCAATCTGGTCCTTCATCTTTTTAAGCCCGCAATCAACGCTCTCC‑AGAGTCCGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGCTGTTCACCTCAGAGAGGATGGCCGAAC‑AGAAAAACAAAAATTCAC‑CTTTGTTTTTGAACCTAGAGGGTCCACGGGAGTAAAAAATTATTGTGACCATTTTGCCGGTGACTCAATAAGGGAGGTGTGGGACGc                                                                                                                     >  1:712969/1‑251 (MQ=255)                                                                                                                     cGGTTCCAAAAACACAGGCGGGAACCAATCGGGTCATGCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAGTATGGTGACCATTTTACCGTTGCCTCAATAAGGCAGATGTGGGACGCAGCg                                                                                                                 <  2:792383/251‑1 (MQ=255)                                                                                                                          ccAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAAcc                                                                                                                                                                                             >  1:22506/1‑170 (MQ=255)                                                                                                                          ccAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAAcc                                                                                                                                                                                             <  2:22506/170‑1 (MQ=255)                                                                                                                           cAAAAACACAGGCGGGAACCAAGCGGGTCATTCATCTTAGTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGTTGTGGGACGCAGCGATaaaa                                                                                                           <  2:252091/251‑1 (MQ=255)                                                                                                                            aaaaaCACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAAc                                                                                                          <  2:308498/251‑1 (MQ=255)                                                                                                                                           aaCCAATCGGGTCATTCATCTTATTAAGCCAGAAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACCAAAGAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGTACTCCGcc                                                                                           >  1:187657/1‑251 (MQ=255)                                                                                                                                               aaTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGGCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg                                                                                       <  2:259313/251‑1 (MQ=255)                                                                                                                                                      tcattcatCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTATCGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAA‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGAAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATc                                                                                 >  2:665238/1‑250 (MQ=255)                                                                                                                                                                       ccAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGAAAAAAATTATGGTGACCATTTTACCGATGACTCAATACGGCAGATGTGGGACGCAGCGATAAAACGTGACGGACTACGCCATCGAAATTCATATCAGTCGatacatac                                                               >  2:707165/1‑251 (MQ=255)                                                                                                                                                                              tCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTAttcc                                                        >  2:308316/1‑248 (MQ=255)                                                                                                                                                                                                                gaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGc                        >  1:542912/1‑250 (MQ=255)                                                                                                                                                                                                                            agCATAACATTGGTGTTAACCTCAGAGCGTATGGCCGAAC‑AGAAAAACAAAAATGAAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTATACCGTTGACTCAATGAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACACCGCACTCGAAAATAATATCAGTAGAAACATAATTCTGCCTGCTGGTCTCTGACAACTggagttaacagggactttagagcaaac           >  2:776440/1‑226 (MQ=255)                                                                                                                                                                                                                               atatCATTGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATTGTGACCAGTTTACCGTTGACTCAATAAGGCATATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAg        <  1:45933/251‑1 (MQ=255)                                                                                                                                                                                                                                     ttGATGTTCACCTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGc  >  1:246395/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  GTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAG‑GACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCC‑GGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAAC‑AGAAAAACAAAAATGCAC‑CTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGC  >  NC_000913/1652094‑1652570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑