Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,485,638	T→A	L422* (TTA→TAA)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,485,638	0	T	A	100.0%	107.6 / NA	30	L422* (TTA→TAA)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/14);  total (16/14)

TTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATTAAT  >  NC_000913/2485400‑2485873                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            ttttCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTAt                                                                                                                                                                                                                                >  2:229660/1‑251 (MQ=255)   ttCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATcc                                                                                                                                                                                                                              >  1:301623/1‑251 (MQ=255)   ttCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACAGTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATc                                                                                                                                                                                                                               <  1:74328/250‑1 (MQ=255)     ctcCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTAAAGCCTTCATAACAACGCCTTACGCTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAACACGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTTTCCgg                                                                                                                                                                                                                            >  2:17578/1‑251 (MQ=255)             aCCGTTTCAAACAATATCCATGCTGGCACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTCTTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGACTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGa                                                                                                                                                                                                                     >  2:782373/1‑250 (MQ=255)                                                      cGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGt                                                                                                                                                                           >  1:159830/1‑251 (MQ=255)                                                                       taCCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTg                                                                                                                                                          >  1:99609/1‑251 (MQ=255)                                                                                 tATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGt                                                                                                                                                >  1:412350/1‑251 (MQ=255)                                                                                    ttATAGTGAAGATCGAGAAAATAATGTTTGATTTGCTGAATCCTTCATAACAACGCCGTAAGGTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCGCTGGTcg                                                                                                                                              <  2:524026/250‑1 (MQ=255)                                                                                        aatGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTCTCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTAATGCTCTGGTCGCGac                                                                                                                                          >  2:575730/3‑250 (MQ=255)                                                                                        aGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGac                                                                                                                                          >  1:13746/1‑250 (MQ=255)                                                                                               aacGAGAAAATAATGTTTTAGTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTaa                                                                                                                                  <  2:12883/249‑1 (MQ=255)                                                                                                      aaaTAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACGTGATGCTCTGGTCGCGACACAGCTAAATTcgcg                                                                                                                           <  1:703870/251‑1 (MQ=255)                                                                                                           atGTTTTTTTTGTTGAAGTCTTCATAAAAACGCCTTACGTTTTTTTCATTCAAAAAGCGCCTTACAGTTAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATTATGCCAGCGCTGCATTTCACAATTTTAAGGAAGGTGAACTGGAGGCTCTGGTCGCGACACAGCTAAATTCGCGTTACa                                                                                                                      <  2:671093/251‑1 (MQ=255)                                                                                                                           aGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGGTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGTTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTa                                                                                                      <  2:99609/251‑1 (MQ=255)                                                                                                                           aGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGATGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGAGCGATCATTACt                                                                                                       >  2:469213/1‑250 (MQ=255)                                                                                                                                tCATAACAACGCCTTACGGTTGTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATGCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGATGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCt                                                                                                  <  2:668153/250‑1 (MQ=255)                                                                                                                                  aTAACAACGTCTTACGTTTTTGTCAGGCAAAAAGCGCGTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGCCGCTATTCCATATTATGATGAGCTGCATTCG‑TAGAAAAATAGATGTATCCGGATGTTGAACGGATACAGGTCGATAATGCCAGCGCTGCATTTTACAAGGTTAAGGAAGGTGAACTTTATGCTCTGGTCGCGATACTGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAAt                                                                                               <  1:452632/251‑1 (MQ=255)                                                                                                                                        acGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACtt                                                                                         <  1:489011/251‑1 (MQ=255)                                                                                                                                           ccTTACGTTTTTGGCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGAATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTAt                                                                                      <  2:785379/251‑1 (MQ=255)                                                                                                                                                 gTTTTTGTCATGCAAAAAGCGCCTGAAAGAGAACCAACATTAAAAAAAAGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCAtttt                                                                                >  1:252144/1‑251 (MQ=255)                                                                                                                                                             cAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGc                                                                    >  1:586956/1‑251 (MQ=255)                                                                                                                                                                    cgcCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCg                                                              >  2:665401/1‑250 (MQ=255)                                                                                                                                                                       cTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATg                                                          >  1:391962/1‑251 (MQ=255)                                                                                                                                                                                      caTTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTttcgttcg                                           <  1:39064/251‑1 (MQ=255)                                                                                                                                                                                           aaaaaaGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCtttt                                      <  2:13746/251‑1 (MQ=255)                                                                                                                                                                                              aaaGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCt                                   <  2:301623/251‑1 (MQ=255)                                                                                                                                                                                              aaaGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTCTTCCt                                   <  2:159830/251‑1 (MQ=255)                                                                                                                                                                                                     tGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGATTGCATCGCTTTCGTTCGCTTTTCCTCGCgg                              >  1:491599/1‑249 (MQ=255)                                                                                                                                                                                                                                aGCTGCATTCGCA‑TAAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATtaat  >  2:305731/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            TTTTCTCCGATCACCGTTTCACACAATATCCATGCTGGAACACAGCTTAGCCCCGGAGGATGGGATATAATACCTGGCGCTATTTATAGTGAAGATCGAGAAAATAATGTTTTATTTGCTGAAGCCTTCATAACAACGCCTTACGTTTTTGTCATGCAAAAAGCGCCTGACAGTGAACAAACATTAAAAAAAGGAATGAAAGTTGCCATTCCATATTATTATGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATTAAT  >  NC_000913/2485400‑2485873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑